##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781201_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	186080
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.403450128976782	34.0	33.0	34.0	23.0	34.0
2	31.553192175408427	34.0	33.0	34.0	24.0	34.0
3	31.485715821152194	34.0	33.0	34.0	23.0	34.0
4	31.65924871023216	34.0	34.0	34.0	23.0	34.0
5	31.6781975494411	34.0	34.0	34.0	23.0	34.0
6	34.525	38.0	35.0	38.0	26.0	38.0
7	34.4801913155632	38.0	34.0	38.0	25.0	38.0
8	34.33595227858985	37.0	34.0	38.0	24.0	38.0
9	34.57526332760103	38.0	35.0	38.0	26.0	38.0
10-11	34.42970496560619	38.0	34.0	38.0	25.0	38.0
12-13	34.372116831470336	38.0	34.0	38.0	25.0	38.0
14-15	34.23613230868443	37.5	34.0	38.0	24.0	38.0
16-17	34.192994948409286	37.0	34.0	38.0	24.0	38.0
18-19	34.268188413585555	37.0	34.0	38.0	24.5	38.0
20-21	34.19218078245916	37.0	34.0	38.0	24.0	38.0
22-23	34.24067067927773	37.0	34.0	38.0	24.5	38.0
24-25	34.241968508168526	37.0	34.0	38.0	24.0	38.0
26-27	34.14989789337919	37.0	34.0	38.0	24.0	38.0
28-29	34.22609898968186	37.0	34.0	38.0	24.0	38.0
30-31	34.31026171539123	37.0	34.0	38.0	24.5	38.0
32-33	34.25730062338779	37.0	34.0	38.0	24.0	38.0
34-35	34.27881556319863	37.0	34.0	38.0	24.0	38.0
36-37	34.136191423043854	37.0	34.0	38.0	24.0	38.0
38-39	34.16074000429923	37.0	34.0	38.0	24.0	38.0
40-41	34.130317605331044	37.0	34.0	38.0	24.0	38.0
42-43	34.21679385210662	37.0	34.0	38.0	24.0	38.0
44-45	34.233155094582976	37.0	34.0	38.0	24.0	38.0
46-47	34.214813521066205	37.0	34.0	38.0	24.0	38.0
48-49	34.18756717540843	37.0	34.0	38.0	24.0	38.0
50-51	34.06847861134996	37.0	34.0	38.0	23.5	38.0
52-53	34.162800945829744	37.0	34.0	38.0	24.0	38.0
54-55	34.11191154342218	37.0	34.0	38.0	24.0	38.0
56-57	34.09069486242476	37.0	34.0	38.0	24.0	38.0
58-59	34.1066557394669	37.0	34.0	38.0	24.0	38.0
60-61	34.07629245485813	37.0	34.0	38.0	24.0	38.0
62-63	34.04690724419605	37.0	34.0	38.0	24.0	38.0
64-65	34.05931319862425	37.0	34.0	38.0	24.0	38.0
66-67	33.94469582975064	37.0	34.0	38.0	23.0	38.0
68-69	33.724916702493545	37.0	34.0	38.0	22.5	38.0
70-71	33.85353611349957	37.0	34.0	38.0	23.0	38.0
72-73	33.80793475924334	37.0	34.0	38.0	23.0	38.0
74-75	33.76534286328461	37.0	34.0	38.0	23.0	38.0
76	32.38115864144454	37.0	31.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	22.0
14	92.0
15	223.0
16	516.0
17	905.0
18	1272.0
19	1421.0
20	1366.0
21	1416.0
22	1547.0
23	1801.0
24	2103.0
25	2484.0
26	2953.0
27	3510.0
28	4075.0
29	4893.0
30	5950.0
31	7174.0
32	8829.0
33	10867.0
34	13915.0
35	18481.0
36	28226.0
37	62037.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.274290627687016	17.96969045571797	9.448624247635426	25.30739466895959
2	23.87736457437661	20.11822871883061	30.910898538263115	25.093508168529667
3	20.06932502149613	22.083512467755803	29.02998710232158	28.817175408426483
4	14.511500429922613	16.228503869303527	36.71216680997421	32.54782889079966
5	14.1621883061049	33.668314703353396	35.30900687876182	16.86049011177988
6	30.035468615649187	36.66111349957008	18.507093723129834	14.796324161650903
7	29.42659071367154	29.15627687016337	21.847055030094584	19.57007738607051
8	26.482158211521927	33.64090713671539	19.66627257093723	20.21066208082545
9	25.334264832330184	15.858770421324161	21.205932932072226	37.60103181427343
10-11	25.90713671539123	24.04664660361135	27.344959157351678	22.701257523645744
12-13	26.108394239036976	22.392519346517627	26.19733447979364	25.30175193465176
14-15	24.019776440240754	23.73280309544282	23.645206362854687	28.602214101461737
16-17	24.234737747205504	26.20243981083405	25.032512897678416	24.530309544282026
18-19	23.608931642304384	25.980223559759242	26.11215606190886	24.298688736027515
20-21	23.512467755803957	26.30374032674119	26.01730438521066	24.166487532244197
22-23	24.088564058469476	25.625268701633708	26.329804385210664	23.956362854686155
24-25	23.67718185726569	25.98559759243336	25.993927343078244	24.3432932072227
26-27	22.856029664660362	26.15595442820292	26.48188950988822	24.506126397248494
28-29	23.772839638865005	25.763381341358553	26.98194325021496	23.481835769561478
30-31	23.891068357695612	25.792401117798796	26.47785898538263	23.838671539122956
32-33	23.14864574376612	26.371184436801375	26.37091573516767	24.109254084264833
34-35	23.982964316423043	25.418637145313845	26.578084694754946	24.020313843508166
36-37	24.12994411006019	25.406008168529663	26.109737747205504	24.354309974204643
38-39	23.316046861564917	25.982373172828893	26.967433361994843	23.73414660361135
40-41	23.522947119518488	25.127901977644022	27.38150257953568	23.967648323301805
42-43	23.26176913155632	25.216842218400686	27.073033104041272	24.44835554600172
44-45	22.622588732235414	25.234106927415432	27.231640239789982	24.911664100559168
46-47	22.956792777300087	24.883383490971624	27.597538693035258	24.562285038693034
48-49	22.35275150472915	24.646657351676698	28.372474204643165	24.62811693895099
50-51	22.011500429922613	24.92261392949269	27.966734737747206	25.09915090283749
52-53	21.56841143594153	24.78530739466896	28.60167669819432	25.044604471195186
54-55	21.308308254514188	25.26950773860705	28.55760963026655	24.864574376612207
56-57	20.97588108212685	25.284558088543513	28.478648738700972	25.26091209062866
58-59	21.147738326194787	25.109765207250685	28.12246411470397	25.620032351850558
60-61	21.118775140599265	24.89245130415389	28.10023726545517	25.888536289791674
62-63	20.559389073399327	24.465815410741733	28.76428164533153	26.210513870527414
64-65	21.404675983523084	24.729616801511188	27.849805324097087	26.015901890868637
66-67	21.393390423933987	24.60547564603111	27.315353466413367	26.685780463621533
68-69	21.119906705790044	24.909715280688744	27.110136609378866	26.86024140414235
70-71	21.897210048447295	24.616761205620207	26.76827253014185	26.71775621579065
72-73	21.794892464450392	24.367200851255923	26.69095755543374	27.146949128859944
74-75	21.686873246702994	24.556368834574748	26.55956104429325	27.197196874429004
76	21.879836629406707	24.178847807394668	26.583190025795357	27.358125537403268
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.0
12	2.0
13	2.5
14	6.5
15	12.5
16	31.0
17	47.0
18	54.5
19	80.0
20	130.0
21	162.0
22	208.5
23	354.5
24	515.5
25	577.0
26	681.0
27	874.0
28	1198.5
29	1434.0
30	1584.5
31	1959.5
32	2659.0
33	3134.0
34	3431.5
35	4231.5
36	5333.5
37	5933.0
38	6416.5
39	7578.0
40	8927.5
41	10059.5
42	10520.0
43	10863.5
44	11323.5
45	11299.0
46	11158.0
47	11102.0
48	10372.5
49	9231.5
50	8764.0
51	8217.0
52	7007.5
53	5964.0
54	5583.0
55	5086.0
56	4249.5
57	3656.0
58	3402.0
59	3066.5
60	2534.5
61	2343.5
62	2349.0
63	2267.5
64	2081.0
65	1904.5
66	1858.5
67	1884.0
68	1780.5
69	1697.0
70	1725.5
71	1734.0
72	1641.0
73	1538.5
74	1442.5
75	1356.0
76	1243.5
77	1070.5
78	905.5
79	801.0
80	678.0
81	507.5
82	400.0
83	340.0
84	276.0
85	180.5
86	117.0
87	85.0
88	62.0
89	25.5
90	11.0
91	7.5
92	5.0
93	4.5
94	2.5
95	0.5
96	0.0
97	0.5
98	0.5
99	1.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	2.6870163370593293E-4
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0010748065348237317
58-59	5.374032674118659E-4
60-61	8.061049011177987E-4
62-63	0.0010748065348237317
64-65	8.061049011177987E-4
66-67	8.061049011177987E-4
68-69	0.0010748065348237317
70-71	8.061049011177987E-4
72-73	0.0010748065348237317
74-75	0.0010748065348237317
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	186080.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.04546431642304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.60890311730678	37.985275150472916
2	14.274570192590192	15.14402407566638
3	5.753391350157537	9.155739466895959
4	2.4962768597971774	5.29664660361135
5	1.3383042742662628	3.549548581255374
6	0.8398593818067615	2.673043852106621
7	0.6412919043228951	2.3812338779019777
8	0.44981612246345243	1.9088564058469475
9	0.40827904809182736	1.9491616509028376
>10	2.1700588610736826	19.35565348237317
>50	0.018235788748518344	0.5465391229578676
>100	0.0010130993749176856	0.05427773000859845
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0021496130696474634	0.0
2	0.0	0.0	0.0	0.0021496130696474634	0.0
3	0.0	0.0	0.0	0.0021496130696474634	0.0
4	0.0	0.0	0.0	0.0021496130696474634	0.0
5	0.0	0.0	0.0	0.0021496130696474634	0.0
6	0.0	0.0	0.0	0.0021496130696474634	0.0
7	0.0	0.0	0.0	0.0021496130696474634	0.0
8	0.0	0.0	0.0	0.0021496130696474634	0.0
9	0.0	0.0	0.0	0.0021496130696474634	0.0
10	0.0	0.0	0.0	0.002687016337059329	0.0
11	0.0	0.0	0.0	0.002687016337059329	0.0
12	0.0	0.0	0.0	0.002687016337059329	0.0
13	0.0	0.0	0.0	0.002687016337059329	0.0
14	0.0	0.0	0.0	0.004836629406706793	0.0
15	0.0	0.0	0.0	0.004836629406706793	0.0
16	0.0	0.0	0.0	0.005374032674118658	0.0
17	0.0	0.0	0.0	0.005374032674118658	0.0
18	0.0	0.0	0.0	0.005911435941530524	0.0
19	0.0	0.0	0.0	0.0069862424763542565	0.0
20	0.0	0.0	0.0	0.0069862424763542565	0.0
21	0.0	0.0	0.0	0.007523645743766122	0.0
22	0.0	0.0	0.0	0.008061049011177988	0.0
23	0.0	0.0	0.0	0.008061049011177988	0.0
24	0.0	0.0	0.0	0.008598452278589854	0.0
25	0.0	5.374032674118659E-4	0.0	0.010748065348237317	0.0
26	0.0	5.374032674118659E-4	0.0	0.013435081685296647	0.0
27	0.0	5.374032674118659E-4	0.0	0.024720550300945828	0.0
28	0.0	5.374032674118659E-4	0.0	0.03546861564918315	0.0
29	0.0	5.374032674118659E-4	0.0	0.048366294067067925	0.0
30	0.0	5.374032674118659E-4	0.0	0.06932502149613069	0.0
31	0.0	5.374032674118659E-4	0.0	0.0913585554600172	0.0
32	0.0	5.374032674118659E-4	0.0	0.11607910576096303	0.0
33	0.0	5.374032674118659E-4	0.0	0.16283319002579535	0.0
34	0.0	5.374032674118659E-4	0.0	0.22570937231298366	0.0
35	0.0	5.374032674118659E-4	0.0	0.33319002579535684	0.0
36	0.0	5.374032674118659E-4	0.0	0.5046216680997421	0.0
37	0.0	5.374032674118659E-4	0.0	0.7545141874462596	0.0
38	0.0	5.374032674118659E-4	0.0	1.0699699054170249	0.0
39	0.0	5.374032674118659E-4	0.0	1.4466895958727428	0.0
40	0.0	5.374032674118659E-4	0.0	1.8819862424763543	0.0
41	0.0	5.374032674118659E-4	0.0	2.3285683576956147	0.0
42	0.0	5.374032674118659E-4	0.0	2.8176053310404128	0.0
43	0.0	5.374032674118659E-4	0.0	3.4211092003439383	0.0
44	0.0	5.374032674118659E-4	0.0	4.155739466895959	0.0
45	0.0	5.374032674118659E-4	0.0	4.983877901977644	0.0
46	0.0	5.374032674118659E-4	0.0	5.88993981083405	0.0
47	0.0	5.374032674118659E-4	0.0	6.796001719690456	0.0
48	0.0	5.374032674118659E-4	0.0	7.74183147033534	0.0
49	0.0	5.374032674118659E-4	0.0	8.594153052450558	0.0
50	0.0	5.374032674118659E-4	0.0	9.345442820292348	0.0
51	0.0	5.374032674118659E-4	0.0	10.091358555460017	0.0
52	0.0	5.374032674118659E-4	0.0	10.873280309544281	0.0
53	0.0	5.374032674118659E-4	0.0	11.643916595012898	0.0
54	0.0	5.374032674118659E-4	0.0	12.460769561478934	0.0
55	0.0	5.374032674118659E-4	0.0	13.293744625967326	0.0
56	0.0	5.374032674118659E-4	0.0	14.118121238177128	0.0
57	0.0	5.374032674118659E-4	0.0	14.98549011177988	0.0
58	0.0	5.374032674118659E-4	0.0	15.845335339638865	0.0
59	0.0	5.374032674118659E-4	0.0	16.631018916595014	0.0
60	0.0	5.374032674118659E-4	0.0	17.427450558899398	0.0
61	0.0	5.374032674118659E-4	0.0	18.163693035253655	0.0
62	0.0	5.374032674118659E-4	0.0	18.905846947549442	0.0
63	0.0	5.374032674118659E-4	0.0	19.59372312983663	0.0
64	0.0	5.374032674118659E-4	0.0	20.343400687876183	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTCG	20	7.9111895E-5	70.00001	15
GTTTCGT	25	2.3858136E-4	56.000004	16
CCCGTTT	25	2.3858136E-4	56.000004	13
CTTGATA	20	0.0069429274	52.500004	24
AACTCTC	30	5.8666355E-4	46.666668	19
CCGTTTC	30	5.8666355E-4	46.666668	14
CGTGCAA	35	0.0012530582	40.000004	20
GATCTAA	35	0.0012530582	40.000004	15
TAGCGAG	45	9.172193E-5	38.88889	28
ATCCCGT	45	9.172193E-5	38.88889	11
ACGTTCT	40	0.0024142424	35.000004	39
ATCGTGA	40	0.0024142424	35.000004	13
GTGCTAG	40	0.0024142424	35.000004	27
CAAGAAT	40	0.0024142424	35.000004	24
GCTTTAA	40	0.0024142424	35.000004	1
GATCCCG	65	2.0946136E-5	32.307693	10
CTTGCTA	55	2.9838886E-4	31.818184	36
CGAGGTT	55	2.9838886E-4	31.818184	22
CATGTAC	55	2.9838886E-4	31.818184	3
GTGCAAG	45	0.0042992746	31.111113	21
>>END_MODULE