FastQCFastQC Report
Wed 25 May 2016
SRR1781201_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781201_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168345
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT25131.4927678279723187No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG3330.19780807270783213No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC2540.1508806320354035No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGA207.9090016E-570.032
AACTCGT150.002221597170.037
CTCGTAA252.3851567E-456.00000430
AATCTCG2950.054.5762736
CGTATGC3700.053.9189241
TATGCCG3700.053.9189243
TCGTATG3700.052.97297340
CCGTCTT3900.052.9487247
GCCGTCT3900.052.9487246
ATGCCGT3850.052.72727244
AAACTCG200.006941783252.536
TTGGTCG200.006941783252.510
CGAGCAA200.006941783252.537
ATACATG200.006941783252.58
CTCGTAT3750.052.2666739
CGTCTTC4050.050.9876548
GTCTTCT4000.050.75000449
ATCTCGT3250.050.61538337
TCTCGTA3200.050.312538
CTTGAAA3700.049.1891957
Produced by FastQC (version 0.11.5)