FastQCFastQC Report
Wed 25 May 2016
SRR1781200_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781200_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306986
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCGG608.367351E-1152.522
AACCGGC651.8553692E-1048.46153623
CGGCTTT757.530616E-1042.026
ACCAGCG459.1861475E-538.88888512
CACTAAG400.002416680635.00000436
CCATAGG400.002416680635.0000041
ACCGGCT1055.475158E-1033.33333624
AAGAATT1357.2759576E-1231.1111155
ATTCGCA450.00430359231.11110937
TATGCGA450.00430359231.11110970
GGCTTTT1052.0163498E-830.00000227
CTAACCG1301.6552804E-1029.61538321
TTTTTGC1103.169407E-828.63636230
CATTATC1103.169407E-828.63636247
GGCCATT1103.169407E-828.63636244
ATTATCG1103.169407E-828.63636248
ATCGAAG1103.169407E-828.63636251
AGCGTTA500.00720226827.99999810
TGCCAAC500.00720226827.99999845
GCTCTAT500.00720226827.9999981
Produced by FastQC (version 0.11.5)