##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781200_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	306986
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.484611676102492	34.0	33.0	34.0	23.0	34.0
2	31.53273113431883	34.0	33.0	34.0	24.0	34.0
3	31.451163896724932	34.0	33.0	34.0	23.0	34.0
4	31.625741890509666	34.0	34.0	34.0	23.0	34.0
5	31.686770732215802	34.0	34.0	34.0	23.0	34.0
6	34.54980357410436	38.0	34.0	38.0	26.0	38.0
7	34.42363495403699	37.0	34.0	38.0	25.0	38.0
8	34.19746177350107	37.0	34.0	38.0	24.0	38.0
9	34.50058308847961	38.0	34.0	38.0	26.0	38.0
10-11	34.36793860306334	37.5	34.0	38.0	25.0	38.0
12-13	34.33060302424215	37.0	34.0	38.0	24.5	38.0
14-15	34.21834546200804	37.0	34.0	38.0	24.0	38.0
16-17	34.19228238421296	37.0	34.0	38.0	24.5	38.0
18-19	34.260643807860944	37.0	34.0	38.0	24.5	38.0
20-21	34.185845608594526	37.0	34.0	38.0	24.0	38.0
22-23	34.26102167525555	37.0	34.0	38.0	24.5	38.0
24-25	34.2473044373359	37.0	34.0	38.0	24.0	38.0
26-27	34.17141986931	37.0	34.0	38.0	24.0	38.0
28-29	34.26380518981321	37.0	34.0	38.0	24.0	38.0
30-31	34.34462483631175	37.0	34.0	38.0	25.0	38.0
32-33	34.27569335409432	37.0	34.0	38.0	24.5	38.0
34-35	34.30500250825771	37.0	34.0	38.0	24.0	38.0
36-37	34.15471389574769	37.0	34.0	38.0	24.0	38.0
38-39	34.16911520395067	37.0	34.0	38.0	24.0	38.0
40-41	34.152754523007566	37.0	34.0	38.0	24.0	38.0
42-43	34.2221339083867	37.0	34.0	38.0	24.0	38.0
44-45	34.25533249073247	37.0	34.0	38.0	24.5	38.0
46-47	34.24912862475813	37.0	34.0	38.0	24.0	38.0
48-49	34.212991797671556	37.0	34.0	38.0	24.0	38.0
50-51	34.0782234368994	37.0	34.0	38.0	24.0	38.0
52-53	34.16889369547797	37.0	34.0	38.0	24.0	38.0
54-55	34.134411666981556	37.0	34.0	38.0	24.0	38.0
56-57	34.10136455734138	37.0	34.0	38.0	24.0	38.0
58-59	34.11375111568606	37.0	34.0	38.0	24.0	38.0
60-61	34.078897734750115	37.0	34.0	38.0	24.0	38.0
62-63	34.05579407529985	37.0	34.0	38.0	24.0	38.0
64-65	34.067377665430996	37.0	34.0	38.0	24.0	38.0
66-67	33.9658355755637	37.0	34.0	38.0	24.0	38.0
68-69	33.7427407113028	37.0	34.0	38.0	22.5	38.0
70-71	33.87752210198511	37.0	34.0	38.0	23.0	38.0
72-73	33.82481448665412	37.0	34.0	38.0	23.0	38.0
74-75	33.81064771683399	37.0	34.0	38.0	23.0	38.0
76	32.39803443805255	37.0	31.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	8.0
13	27.0
14	94.0
15	298.0
16	741.0
17	1367.0
18	1879.0
19	2060.0
20	2065.0
21	2180.0
22	2421.0
23	2856.0
24	3615.0
25	4223.0
26	5009.0
27	5896.0
28	7187.0
29	8588.0
30	10129.0
31	12490.0
32	15074.0
33	18250.0
34	23180.0
35	30424.0
36	46475.0
37	100449.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.83438332692696	18.570227958278227	9.8665737199742	24.72881499482061
2	24.084811685223432	21.084674871166765	30.476959861361756	24.35355358224805
3	20.064758653489083	22.256389542194107	29.143022808857733	28.535828995459077
4	14.3752483826624	17.219352022567804	37.062276455603836	31.343123139165957
5	14.349188562344864	33.30086714052107	35.90065996494955	16.44928433218453
6	28.826395991999636	37.486074283517816	19.01747962447799	14.670050100004561
7	28.857016280872745	29.44108200373958	22.432944824845432	19.268956890542242
8	25.924309251887706	34.05790492074557	19.8347807391868	20.183005088179918
9	25.06954714547243	17.166580886424786	21.637468809652557	36.12640315845022
10-11	25.648400904275764	24.81839562716215	27.01898457910133	22.514218889460757
12-13	25.91518831477657	22.954629852827164	26.028059911526906	25.102121920869358
14-15	23.97894366518343	23.91314261888164	23.679744353162686	28.428169362772245
16-17	24.07487637872737	26.595349625064337	25.236818617135636	24.092955379072663
18-19	23.294710507971047	26.398597981666917	26.543067110552272	23.763624399809764
20-21	23.314255373209203	26.549907813385627	26.431987126448764	23.703849686956406
22-23	23.833985914667117	26.005909064257004	26.683953014143967	23.476152006931912
24-25	23.374192959939542	26.195494257067097	26.651378238747043	23.778934544246315
26-27	22.841106760568884	26.461467298182978	26.71929664544963	23.978129295798507
28-29	23.33819783312594	26.179532617122604	27.48545536278527	22.99681418696618
30-31	23.61052295544422	26.09320946232076	26.880541783664402	23.41572579857062
32-33	22.974989087450243	26.865394513104835	26.494530695210855	23.66508570423407
34-35	23.974871818258812	25.895480575661427	26.93901350550188	23.190634100577874
36-37	23.869818167603736	25.722182770549797	26.438013459897192	23.969985601949276
38-39	22.99616269145824	26.412279387333626	27.226811646133697	23.36474627507443
40-41	23.401392897395972	25.443505567029117	27.43919918172164	23.71590235385327
42-43	22.996325565335226	25.53096883896985	27.259386421530625	24.213319174164294
44-45	22.529366160020324	25.52934010020001	27.502719993745643	24.43857374603402
46-47	22.76781351592581	24.9957652791984	27.84898985621494	24.38743134866085
48-49	22.03683555601884	25.051793892881108	28.537620608105907	24.373749942994145
50-51	22.134071260578658	25.183884607115637	28.264155368648737	24.417888763656975
52-53	21.673952558097113	25.107659632686836	28.680298124344432	24.538089684871622
54-55	21.207970396044118	25.525268253275396	28.630295844110154	24.63646550657033
56-57	21.18011896307975	25.62071234518838	28.46758484100252	24.73158385072935
58-59	21.371495768536676	25.447088792322774	28.07896777051722	25.102447668623324
60-61	20.93580814760282	25.243496446092006	28.35748210016092	25.463213306144254
62-63	20.635794466197154	24.996253900829355	28.644465871407814	25.723485761565673
64-65	21.52085111373157	24.997231144091263	27.825698891806137	25.656218850371026
66-67	21.336640758861968	25.154404435381416	27.437896190705764	26.071058615050852
68-69	21.432084850774956	25.02052210850006	27.09814128331585	26.44925175740913
70-71	21.95181539223287	24.938596548376797	26.96784218172816	26.141745877662174
72-73	21.81988755187533	24.673112128891873	26.82939938629123	26.67760093294157
74-75	21.572645006612678	24.867583538011505	26.949763181382863	26.610008273992953
76	21.548865420572923	24.76432150000326	26.96930804662102	26.717505032802798
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	2.0
12	3.0
13	2.0
14	2.5
15	9.0
16	30.0
17	46.0
18	53.5
19	84.5
20	155.5
21	203.0
22	256.5
23	383.5
24	553.5
25	650.0
26	827.0
27	1162.0
28	1580.0
29	1840.0
30	2144.0
31	2818.0
32	3935.5
33	4683.0
34	5317.5
35	6729.0
36	8489.5
37	9473.0
38	10569.5
39	13147.5
40	15836.5
41	18001.5
42	18959.0
43	19144.0
44	19816.0
45	20407.0
46	20511.0
47	20286.0
48	18870.5
49	16949.0
50	16218.0
51	14920.0
52	12273.5
53	10164.5
54	9404.0
55	8638.0
56	6995.0
57	5571.5
58	5025.0
59	4428.5
60	3563.5
61	3040.5
62	2786.0
63	2738.5
64	2540.5
65	2380.5
66	2331.5
67	2292.0
68	2306.0
69	2288.0
70	2176.0
71	2096.0
72	2064.0
73	1965.0
74	1812.5
75	1727.0
76	1621.0
77	1415.5
78	1205.0
79	1094.0
80	939.5
81	676.5
82	467.0
83	366.0
84	335.0
85	232.0
86	133.5
87	107.0
88	86.5
89	47.5
90	21.5
91	14.5
92	15.0
93	9.5
94	3.5
95	2.5
96	2.0
97	1.5
98	1.5
99	6.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	306986.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.37224494942918
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.93303456042266	33.356962985916745
2	13.279972271452001	12.316442541867993
3	5.351960826259326	7.445473151851406
4	2.360282352048069	4.378063655159516
5	1.335370640088791	3.096206721023673
6	0.8749048021691287	2.4342779876171226
7	0.6569989756886546	2.132656220251085
8	0.5393729932847047	2.000954925096412
9	0.42422214907431877	1.7704920068862813
>10	3.1246988123620514	27.354839640712964
>50	0.11076879711615885	3.275068744487064
>100	0.008412820034138646	0.4385614191297448
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0032574775396923637	0.0
2	0.0	0.0	0.0	0.003908973047630836	0.0
3	0.0	0.0	0.0	0.003908973047630836	0.0
4	0.0	0.0	0.0	0.006189207325415491	0.0
5	0.0	0.0	0.0	0.006514955079384727	0.0
6	0.0	0.0	0.0	0.006840702833353964	0.0
7	0.0	0.0	0.0	0.0071664505873232	0.0
8	0.0	0.0	0.0	0.007492198341292436	0.0
9	0.0	0.0	0.0	0.008469441603200146	0.0
10	0.0	0.0	0.0	0.008469441603200146	0.0
11	0.0	0.0	0.0	0.008469441603200146	0.0
12	0.0	0.0	0.0	0.008469441603200146	0.0
13	0.0	0.0	0.0	0.008469441603200146	0.0
14	0.0	0.0	0.0	0.008795189357169383	0.0
15	0.0	0.0	0.0	0.009446684865107855	0.0
16	0.0	0.0	0.0	0.009446684865107855	0.0
17	0.0	0.0	0.0	0.009446684865107855	0.0
18	0.0	0.0	0.0	0.009772432619077091	0.0
19	0.0	0.0	0.0	0.009772432619077091	0.0
20	0.0	0.0	0.0	0.010098180373046328	0.0
21	0.0	0.0	0.0	0.011075423634954036	0.0
22	0.0	0.0	0.0	0.013029910158769455	0.0
23	0.0	0.0	0.0	0.013681405666707927	0.0
24	0.0	0.0	0.0	0.014984396682584873	0.0
25	0.0	0.0	0.0	0.01596163994449258	0.0
26	0.0	0.0	0.0	0.017590378714338765	0.0
27	0.0	0.0	0.0	0.02899155010326204	0.0
28	0.0	0.0	0.0	0.04136996475409302	0.0
29	0.0	0.0	0.0	0.05863459571446255	0.0
30	0.0	0.0	0.0	0.0824141817542168	0.0
31	0.0	0.0	0.0	0.10326203800824793	0.0
32	0.0	0.0	0.0	0.12867036281784838	0.0
33	0.0	0.0	0.0	0.1700403275719414	0.0
34	0.0	0.0	0.0	0.24105333793723493	0.0
35	0.0	0.0	0.0	0.3312854657867134	0.0
36	0.0	0.0	0.0	0.4694025134696696	0.0
37	0.0	0.0	0.0	0.7313037076609357	0.0
38	0.0	0.0	0.0	1.0186132266618022	0.0
39	0.0	0.0	0.0	1.3867081886470394	0.0
40	0.0	0.0	0.0	1.7919383945847693	0.0
41	0.0	0.0	0.0	2.246356511371854	0.0
42	0.0	0.0	0.0	2.7669014222146937	0.0
43	0.0	0.0	0.0	3.4154652003674433	0.0
44	0.0	0.0	0.0	4.091391789853609	0.0
45	0.0	0.0	0.0	4.8930570123719	0.0
46	0.0	0.0	0.0	5.735766451890314	0.0
47	0.0	0.0	0.0	6.6087704325278676	0.0
48	0.0	0.0	0.0	7.4645097822050515	0.0
49	0.0	0.0	3.2574775396923637E-4	8.281810896913866	0.0
50	0.0	0.0	3.2574775396923637E-4	8.9750021173604	0.0
51	0.0	0.0	3.2574775396923637E-4	9.666890346791059	0.0
52	0.0	0.0	3.2574775396923637E-4	10.384512648785286	0.0
53	0.0	0.0	3.2574775396923637E-4	11.113536122168437	0.0
54	0.0	0.0	3.2574775396923637E-4	11.839302118011897	0.0
55	0.0	0.0	3.2574775396923637E-4	12.592105177434801	0.0
56	0.0	0.0	3.2574775396923637E-4	13.393444652199124	0.0
57	0.0	0.0	3.2574775396923637E-4	14.169375802153844	0.0
58	0.0	0.0	3.2574775396923637E-4	14.916966897513241	0.0
59	0.0	0.0	3.2574775396923637E-4	15.633286208491592	0.0
60	0.0	0.0	3.2574775396923637E-4	16.354491735779483	0.0
61	0.0	0.0	3.2574775396923637E-4	17.061364361892725	0.0
62	0.0	0.0	3.2574775396923637E-4	17.742502915442397	0.0
63	0.0	0.0	3.2574775396923637E-4	18.44546656850801	0.0
64	0.0	0.0	3.2574775396923637E-4	19.116181193930668	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACCGG	60	8.367351E-11	52.5	22
AACCGGC	65	1.8553692E-10	48.461536	23
CGGCTTT	75	7.530616E-10	42.0	26
ACCAGCG	45	9.1861475E-5	38.888885	12
CACTAAG	40	0.0024166806	35.000004	36
CCATAGG	40	0.0024166806	35.000004	1
ACCGGCT	105	5.475158E-10	33.333336	24
AAGAATT	135	7.2759576E-12	31.11111	55
ATTCGCA	45	0.004303592	31.111109	37
TATGCGA	45	0.004303592	31.111109	70
GGCTTTT	105	2.0163498E-8	30.000002	27
CTAACCG	130	1.6552804E-10	29.615383	21
TTTTTGC	110	3.169407E-8	28.636362	30
CATTATC	110	3.169407E-8	28.636362	47
GGCCATT	110	3.169407E-8	28.636362	44
ATTATCG	110	3.169407E-8	28.636362	48
ATCGAAG	110	3.169407E-8	28.636362	51
AGCGTTA	50	0.007202268	27.999998	10
TGCCAAC	50	0.007202268	27.999998	45
GCTCTAT	50	0.007202268	27.999998	1
>>END_MODULE