FastQCFastQC Report
Wed 25 May 2016
SRR1781200_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781200_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276076
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT36961.3387617902316753No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG3790.13728103855460091No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC2910.10540575783479912No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5950.057.05882343
CGTCTTC5850.056.83760548
CGTATGC6050.056.69421441
TCGTATG6050.056.69421440
CTCGTAT5950.056.47058539
GCCGTCT5850.056.2393146
CCGTCTT5950.055.8823547
ATCTCGT5200.055.86538337
TCTCGTA5250.055.33333238
TCTTCTG6200.053.62903250
ATCCAGC200.006946469752.50000420
GTCGGAT200.006946469752.5000041
ATGCCGT6550.052.36641344
GTATGCC6550.051.8320642
TGCCGTC6500.051.69230745
CTTGAAA6300.051.66666857
CTGCTTG6700.050.14925454
GTCTTCT6650.050.049
TATCTCG5750.049.91304436
TCGTTGT506.178561E-848.99999634
Produced by FastQC (version 0.11.5)