##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781200_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	276076
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.29815340703285	34.0	34.0	34.0	33.0	34.0
2	33.30313391964531	34.0	34.0	34.0	33.0	34.0
3	33.368547066749734	34.0	34.0	34.0	33.0	34.0
4	33.44057433460352	34.0	34.0	34.0	34.0	34.0
5	33.4395057882612	34.0	34.0	34.0	34.0	34.0
6	36.89018965792028	38.0	37.0	38.0	35.0	38.0
7	36.79330329329605	38.0	37.0	38.0	34.0	38.0
8	36.59401034497747	38.0	37.0	38.0	34.0	38.0
9	36.536877526478214	38.0	37.0	38.0	34.0	38.0
10-11	36.47197148611252	38.0	37.0	38.0	34.0	38.0
12-13	36.40404091626944	38.0	37.0	38.0	34.0	38.0
14-15	36.41302757211782	38.0	37.0	38.0	34.0	38.0
16-17	36.358480997986064	38.0	37.0	38.0	34.0	38.0
18-19	36.357291108245555	38.0	37.0	38.0	34.0	38.0
20-21	36.295007534157264	38.0	37.0	38.0	34.0	38.0
22-23	36.3506769874962	38.0	37.0	38.0	34.0	38.0
24-25	36.35344977469972	38.0	37.0	38.0	34.0	38.0
26-27	36.13148553296918	38.0	37.0	38.0	33.0	38.0
28-29	36.12356561236761	38.0	37.0	38.0	33.5	38.0
30-31	36.1533780553181	38.0	37.0	38.0	34.0	38.0
32-33	36.11663454990655	38.0	37.0	38.0	33.0	38.0
34-35	36.13369144728263	38.0	37.0	38.0	33.5	38.0
36-37	36.08605601356148	38.0	37.0	38.0	32.5	38.0
38-39	36.00261159970443	38.0	37.0	38.0	32.0	38.0
40-41	36.06114077283067	38.0	37.0	38.0	32.5	38.0
42-43	36.086190034628146	38.0	37.0	38.0	33.0	38.0
44-45	36.05370622582187	38.0	37.0	38.0	32.0	38.0
46-47	36.07200191251684	38.0	37.0	38.0	33.0	38.0
48-49	36.0531013199264	38.0	37.0	38.0	32.5	38.0
50-51	35.958761355568754	38.0	37.0	38.0	32.0	38.0
52-53	35.98692751271389	38.0	37.0	38.0	32.0	38.0
54-55	36.00382684478187	38.0	37.0	38.0	32.0	38.0
56-57	35.957645720743564	38.0	37.0	38.0	32.0	38.0
58-59	35.957077036757994	38.0	37.0	38.0	32.0	38.0
60-61	35.94245062953679	38.0	37.0	38.0	32.0	38.0
62-63	35.92134593372839	38.0	37.0	38.0	32.0	38.0
64-65	35.86758537504166	38.0	37.0	38.0	32.0	38.0
66-67	35.837021327460555	38.0	37.0	38.0	31.0	38.0
68-69	35.762449470435676	38.0	36.0	38.0	31.0	38.0
70-71	35.64423202306611	38.0	36.0	38.0	31.0	38.0
72-73	35.533655587591824	38.0	36.0	38.0	31.0	38.0
74-75	35.53718541271244	38.0	36.0	38.0	31.0	38.0
76	34.65094032078123	37.0	34.0	38.0	27.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	2.0
21	13.0
22	40.0
23	73.0
24	193.0
25	363.0
26	720.0
27	1228.0
28	2107.0
29	3372.0
30	5297.0
31	7731.0
32	11216.0
33	15665.0
34	22037.0
35	30634.0
36	46521.0
37	128862.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	45.265796374911254	17.933829814978484	10.025138005476752	26.775235804633507
2	23.79417261913386	21.046016314348222	30.360842666512124	24.798968400005794
3	20.093742302844145	22.230110549269043	29.762818933916748	27.91332821397007
4	14.194642055086282	18.58980860342804	36.13533954418349	31.080209797302196
5	13.983106101218503	33.4875179298454	36.130268476796246	16.399107492139844
6	28.671452788362622	38.38001130123589	18.631826018922325	14.316709891479157
7	28.434199278459555	29.458554890682276	21.380706761906143	20.726539068952025
8	25.160825279995365	35.46450977267129	19.403352699981163	19.971312247352177
9	24.47188455352874	17.02864428635593	20.615337805531812	37.88413335458352
10-11	25.041655196395197	25.358959127196854	27.168786855793332	22.430598820614613
12-13	25.178030687202074	22.867616163665076	26.209268462307477	25.745084686825365
14-15	23.200495515727553	23.5246816094119	23.107948535910403	30.166874338950144
16-17	24.337320158217302	27.34826641939176	24.264151900201394	24.050261522189544
18-19	23.84433996435764	26.293122183746505	25.67825526304351	24.184282588852348
20-21	22.728886248714122	26.35614830698793	25.40151987133978	25.513445572958172
22-23	23.216433156087454	26.887704834900532	25.640584476738287	24.255277532273723
24-25	23.947934626696995	26.793527869137485	25.655254350251376	23.60328315391414
26-27	23.167171358611398	27.267491560294992	25.912792129703416	23.652544951390198
28-29	23.79670815282748	26.183369796722644	26.53019458410003	23.489727466349848
30-31	24.20909459713992	25.637324504846493	27.02824584534693	23.125335052666657
32-33	22.336059635752473	27.316391138671957	25.94629739636912	24.40125182920645
34-35	23.113019603297644	25.933076399252382	28.018009533606687	22.93589446384329
36-37	23.189991161853982	26.226111650415103	26.833371970037234	23.75052521769368
38-39	22.6082672887176	26.440183137976497	27.01556817687883	23.93598139642707
40-41	22.54306785088164	25.1916139034179	27.841246613251425	24.424071632449035
42-43	23.518161665628305	25.23689129080398	27.428316840290357	23.81663020327736
44-45	21.94305191324128	26.292941074196964	27.76083397325374	24.003173039308017
46-47	23.118815108883062	24.747714397484753	27.290673582636664	24.842796910995524
48-49	22.42009446674104	24.746627740187485	27.76789724568597	25.0653805473855
50-51	21.40207768875237	24.773975282168678	28.559345977194685	25.264601051884263
52-53	21.03551196047465	24.847867978382766	29.612498007794958	24.50412205334763
54-55	20.483308943913993	25.221678088642257	28.94311711267912	25.351895854764628
56-57	20.963973688404643	25.201574928642838	28.08755560063171	25.746895782320813
58-59	20.31397151509005	25.23345020936264	29.054318376099335	25.39825989944798
60-61	20.91960184876628	25.977448238890737	27.45403439632565	25.64891551601733
62-63	19.55204364015706	26.598653993827785	27.456751039568815	26.39255132644634
64-65	20.02999174140454	27.107571828047348	26.469703994552223	26.392732435995885
66-67	20.15042959185152	26.852569582288936	26.15547892609282	26.84152189976673
68-69	20.095372288790042	27.274554832727222	25.7934409365537	26.836631941929035
70-71	20.77561975687854	26.59122850229647	25.533729842507135	27.099421898317853
72-73	20.823976006606877	25.705783914574248	25.45838826989669	28.011851808922184
74-75	20.945138295252033	25.687129630971185	25.60128370448717	27.766448369289616
76	21.293774178124863	25.54767527782205	25.56614845187557	27.592402092177515
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	4.5
15	14.0
16	28.5
17	38.0
18	57.0
19	87.0
20	110.0
21	122.0
22	195.5
23	348.0
24	481.0
25	535.0
26	723.5
27	1057.0
28	1409.0
29	1616.0
30	1861.0
31	2431.5
32	3473.0
33	4189.0
34	4663.0
35	5788.5
36	7475.0
37	8510.0
38	9268.5
39	11687.5
40	14766.0
41	16962.5
42	17741.0
43	18085.5
44	18427.5
45	18181.0
46	17937.0
47	17789.5
48	16424.5
49	14729.5
50	14252.0
51	13104.0
52	10958.5
53	9299.0
54	8637.0
55	7878.0
56	6303.0
57	4909.0
58	4331.0
59	3822.0
60	3103.0
61	2711.5
62	2530.0
63	2428.0
64	2292.5
65	2240.5
66	2187.0
67	2152.0
68	2128.0
69	2117.5
70	2044.5
71	1958.0
72	1937.0
73	1811.5
74	1646.0
75	1585.0
76	1548.0
77	1321.5
78	1044.0
79	956.0
80	849.5
81	642.0
82	475.0
83	409.0
84	321.0
85	191.0
86	111.5
87	74.0
88	57.0
89	32.0
90	17.0
91	6.5
92	3.0
93	5.0
94	4.5
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	276076.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.66383236944539
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.23329942428107	21.35055928411562
2	19.722762725299578	14.462241328454592
3	8.275076227089079	9.101880229031298
4	3.5759834890791438	5.244370367980087
5	1.898977888798986	3.4811903494104666
6	1.1959856358254126	2.630965012090049
7	0.9929880549021451	2.5484723312855717
8	0.775990664486776	2.2760633314398158
9	0.6549921201190596	2.1613069165817556
>10	4.5059457912184495	30.972499694397378
>50	0.15799809920383986	3.7979607037839354
>100	0.008999891726801004	0.6174115093321891
>500	0.0	0.0
>1k	9.99987969644556E-4	1.3550789420972513
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT	3696	1.3387617902316753	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG	379	0.13728103855460091	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC	291	0.10540575783479912	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.004346629189063881	0.0
2	0.0	0.0	0.0	0.004708848288152537	0.0
3	0.0	0.0	0.0	0.004708848288152537	0.0
4	0.0	0.0	0.0	0.007244381981773135	0.0
5	0.0	0.0	0.0	0.007606601080861791	0.0
6	0.0	0.0	0.0	0.007968820179950448	0.0
7	0.0	0.0	0.0	0.008331039279039104	0.0
8	0.0	0.0	0.0	0.008693258378127762	0.0
9	0.0	0.0	0.0	0.009417696576305075	0.0
10	0.0	0.0	0.0	0.009417696576305075	0.0
11	0.0	0.0	0.0	0.009779915675393732	0.0
12	0.0	0.0	0.0	0.009779915675393732	0.0
13	0.0	0.0	0.0	0.010866572972659702	0.0
14	0.0	0.0	0.0	0.010866572972659702	0.0
15	0.0	0.0	0.0	0.011591011170837016	0.0
16	0.0	0.0	0.0	0.011591011170837016	0.0
17	0.0	0.0	0.0	0.011591011170837016	0.0
18	0.0	0.0	0.0	0.012315449369014329	0.0
19	0.0	0.0	0.0	0.012315449369014329	0.0
20	0.0	0.0	0.0	0.013039887567191643	0.0
21	0.0	0.0	0.0	0.0134021066662803	0.0
22	0.0	0.0	0.0	0.01448876396354627	0.0
23	0.0	0.0	0.0	0.015213202161723583	0.0
24	0.0	0.0	0.0	0.015937640359900897	0.0
25	0.0	0.0	0.0	0.01666207855807821	0.0
26	0.0	0.0	0.0	0.019197612251698807	0.0
27	0.0	0.0	0.0	0.031513061620713136	0.0
28	0.0	0.0	0.0	0.046726263782436724	0.0
29	0.0	0.0	0.0	0.0644749996377809	0.0
30	0.0	0.0	0.0	0.08693258378127762	0.0
31	0.0	0.0	0.0	0.11011460612295165	0.0
32	0.0	0.0	0.0	0.13945435314913285	0.0
33	0.0	0.0	0.0	0.18219620684159435	0.0
34	0.0	0.0	0.0	0.2586244367493009	0.0
35	0.0	0.0	0.0	0.35823468899868155	0.0
36	0.0	0.0	0.0	0.5063823005259421	0.0
37	0.0	0.0	0.0	0.789275416914183	0.0
38	0.0	0.0	0.0	1.106941566814935	0.0
39	0.0	0.0	0.0	1.500673727524305	0.0
40	0.0	0.0	0.0	1.9288167026470973	0.0
41	0.0	0.0	0.0	2.4163636100204293	0.0
42	0.0	0.0	0.0	2.972732146220606	0.0
43	0.0	0.0	0.0	3.6656572827772065	0.0
44	0.0	0.0	0.0	4.372708964198265	0.0
45	0.0	0.0	0.0	5.221026094263898	0.0
46	0.0	0.0	0.0	6.117880583607413	0.0
47	0.0	0.0	0.0	7.036468218896246	0.0
48	0.0	0.0	0.0	7.935133803735203	0.0
49	0.0	0.0	0.0	8.794679725872586	0.0
50	0.0	0.0	0.0	9.534693345310712	0.0
51	0.0	0.0	0.0	10.272533650154305	0.0
52	0.0	0.0	0.0	11.033555977339573	0.0
53	0.0	0.0	0.0	11.807618192092033	0.0
54	0.0	0.0	0.0	12.562845013691883	0.0
55	0.0	0.0	0.0	13.352120430606066	0.0
56	0.0	0.0	0.0	14.182688824816355	0.0
57	0.0	0.0	0.0	15.016879410017532	0.0
58	0.0	0.0	0.0	15.807965922427158	0.0
59	0.0	0.0	0.0	16.575508193396022	0.0
60	0.0	0.0	0.0	17.330010576797694	0.0
61	0.0	0.0	0.0	18.06966197713673	0.0
62	0.0	0.0	0.0	18.77671365855779	0.0
63	0.0	0.0	0.0	19.501151856735103	0.0
64	0.0	0.0	0.0	20.202045813471653	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	595	0.0	57.058823	43
CGTCTTC	585	0.0	56.837605	48
CGTATGC	605	0.0	56.694214	41
TCGTATG	605	0.0	56.694214	40
CTCGTAT	595	0.0	56.470585	39
GCCGTCT	585	0.0	56.23931	46
CCGTCTT	595	0.0	55.88235	47
ATCTCGT	520	0.0	55.865383	37
TCTCGTA	525	0.0	55.333332	38
TCTTCTG	620	0.0	53.629032	50
ATCCAGC	20	0.0069464697	52.500004	20
GTCGGAT	20	0.0069464697	52.500004	1
ATGCCGT	655	0.0	52.366413	44
GTATGCC	655	0.0	51.83206	42
TGCCGTC	650	0.0	51.692307	45
CTTGAAA	630	0.0	51.666668	57
CTGCTTG	670	0.0	50.149254	54
GTCTTCT	665	0.0	50.0	49
TATCTCG	575	0.0	49.913044	36
TCGTTGT	50	6.178561E-8	48.999996	34
>>END_MODULE