FastQCFastQC Report
Wed 25 May 2016
SRR1781199_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781199_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191257
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTGC200.006943223552.562
GTGTTAC350.001253146240.01
TAGACAC400.002414411235.05
TGTATAG400.002414411235.03
GAATAGA400.002414411235.01
GCATGTA400.002414411235.02
GGTCCTA552.9841997E-431.818181
TCCTGAC450.00429957431.1111092
TAAGCAC450.00429957431.11110920
TTAATGC604.9689563E-429.16666656
GCCTTAC500.007195584527.9999981
TAGACTG657.9349696E-426.9230775
TATAGGA657.9349696E-426.9230775
TTGGACA700.001222818625.026
ACCCATC700.001222818625.066
ATTAGGC1052.0291285E-523.3333323
GGTCTAG952.7696794E-422.1052651
ACGCCCG800.002659104321.8755
GCTGCTC800.002659104321.8758
CTAGGAC800.002659104321.8753
Produced by FastQC (version 0.11.5)