##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781199_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	191257
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.520498596129816	34.0	33.0	34.0	23.0	34.0
2	31.57076603732151	34.0	33.0	34.0	24.0	34.0
3	31.51586608594718	34.0	33.0	34.0	23.0	34.0
4	31.62658098788541	34.0	34.0	34.0	23.0	34.0
5	31.637602806694655	34.0	34.0	34.0	23.0	34.0
6	34.43352138745249	37.0	34.0	38.0	26.0	38.0
7	34.41443711864141	37.0	34.0	38.0	25.0	38.0
8	34.21909263451795	37.0	34.0	38.0	24.0	38.0
9	34.49901964372546	38.0	34.0	38.0	26.0	38.0
10-11	34.34765786350303	38.0	34.0	38.0	24.5	38.0
12-13	34.3060358575111	38.0	34.0	38.0	24.0	38.0
14-15	34.16500311099725	37.0	34.0	38.0	24.0	38.0
16-17	34.14104843221425	37.0	34.0	38.0	23.5	38.0
18-19	34.17700267179764	37.0	34.0	38.0	24.0	38.0
20-21	34.12971289939715	37.0	34.0	38.0	23.5	38.0
22-23	34.187182691352476	37.0	34.0	38.0	24.0	38.0
24-25	34.164882853960904	37.0	34.0	38.0	24.0	38.0
26-27	34.08343485467199	37.0	34.0	38.0	24.0	38.0
28-29	34.150159209858984	37.0	34.0	38.0	24.0	38.0
30-31	34.205124518318286	37.0	34.0	38.0	24.0	38.0
32-33	34.158200745593625	37.0	34.0	38.0	24.0	38.0
34-35	34.18051365440219	37.0	34.0	38.0	24.0	38.0
36-37	34.0391305939129	37.0	34.0	38.0	23.5	38.0
38-39	34.03679603883779	37.0	34.0	38.0	24.0	38.0
40-41	34.05319282431493	37.0	34.0	38.0	23.5	38.0
42-43	34.13526825161955	37.0	34.0	38.0	24.0	38.0
44-45	34.16911276450012	37.0	34.0	38.0	24.0	38.0
46-47	34.16390249768636	37.0	34.0	38.0	24.0	38.0
48-49	34.095034430112364	37.0	34.0	38.0	24.0	38.0
50-51	33.98551425568738	37.0	34.0	38.0	23.5	38.0
52-53	34.08116042811505	37.0	34.0	38.0	24.0	38.0
54-55	34.06839226799542	37.0	34.0	38.0	24.0	38.0
56-57	34.03302101360996	37.0	34.0	38.0	24.0	38.0
58-59	34.017486941654425	37.0	34.0	38.0	24.0	38.0
60-61	33.97332123791547	37.0	34.0	38.0	23.5	38.0
62-63	33.98005563195073	37.0	34.0	38.0	23.5	38.0
64-65	33.98928143806501	37.0	34.0	38.0	23.5	38.0
66-67	33.85998159544488	37.0	34.0	38.0	23.0	38.0
68-69	33.60690066245941	37.0	34.0	38.0	22.5	38.0
70-71	33.752725390443224	37.0	34.0	38.0	23.0	38.0
72-73	33.7177096786	37.0	34.0	38.0	23.0	38.0
74-75	33.65514464830045	37.0	34.0	38.0	22.5	38.0
76	32.296548623056935	37.0	31.0	38.0	19.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	28.0
14	114.0
15	327.0
16	704.0
17	1182.0
18	1449.0
19	1529.0
20	1424.0
21	1456.0
22	1445.0
23	1740.0
24	1955.0
25	2459.0
26	2884.0
27	3528.0
28	4131.0
29	5046.0
30	6352.0
31	7410.0
32	9393.0
33	11526.0
34	14803.0
35	19635.0
36	29981.0
37	60754.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	45.99622497477217	17.885881301076562	9.86682840366627	26.251065320485
2	23.407770695974524	19.879010964304573	30.754429903219226	25.958788436501674
3	20.200567822354216	21.39372676555629	28.403666271038446	30.002039141051046
4	14.945335334131563	15.77144888814527	35.14172030304773	34.14149547467544
5	14.756061216060065	33.01892218324035	34.867743402855844	17.35727319784374
6	31.471789267843793	34.90486622711849	18.148355354313832	15.474989150723895
7	30.286473174838047	28.166289338429447	21.42196102626309	20.125276460469422
8	26.922936153970834	33.22231343166525	19.328965737201777	20.525784677162143
9	25.252409062151976	16.166728538040438	20.827472981381074	37.753389418426515
10-11	25.969768426776536	23.90840596684043	26.55562933644259	23.566196269940445
12-13	25.99878697250297	22.296438823154187	26.124795432324042	25.5799787720188
14-15	24.00016731413752	23.450644943714476	23.370647871711885	29.178539870436115
16-17	24.13062005573652	26.323219542291266	24.635961036720225	24.91019936525199
18-19	23.739523259279398	25.767945748390908	26.1253182890038	24.36721270332589
20-21	23.60305766586321	26.132638282520375	25.8267671248634	24.43753692675301
22-23	24.03519871168114	25.602723037588166	26.0089826777582	24.353095572972492
24-25	23.557107863000734	25.806181750685596	25.817684627712	24.81902575860167
26-27	23.279932237774304	25.915652760421843	26.144663986154754	24.6597510156491
28-29	23.437050670040836	25.836962830118637	26.81182910952279	23.91415739031774
30-31	23.66370904071485	25.681935824571127	26.21629535128126	24.438059783432763
32-33	23.331956477409975	25.888202784734677	26.11407687038906	24.66576386746629
34-35	23.93141166074967	25.481158859545012	26.362172364933052	24.22525711477227
36-37	24.188657147189385	25.13947201932478	25.981532702071036	24.690338131414798
38-39	23.184772322058798	25.877222794459808	26.741504886095672	24.196499997385718
40-41	23.61011623103991	25.030717829935632	27.127373115755237	24.231792823269213
42-43	22.87497973930366	24.909676508572236	27.20057305092101	25.014770701203094
44-45	22.650674223688544	24.797000894084924	27.412852862901747	25.13947201932478
46-47	22.72361273051444	24.63073246992267	27.772055401893788	24.873599397669103
48-49	22.14925466780301	24.59125679060113	28.23661356185656	25.022874979739303
50-51	21.998410515693543	24.64589531363558	28.126813659107903	25.228880511562974
52-53	21.585615167026567	24.7972623224248	28.5769932603774	25.040129250171233
54-55	21.110077016788928	25.246396210334787	28.295957795008807	25.347568977867475
56-57	20.980931416889316	25.331621849134933	28.339616327768397	25.347830406207354
58-59	21.195880924308454	24.92908737742247	28.05159563204388	25.823436066225202
60-61	21.07948990102323	24.896082234898593	28.039235165248854	25.985192698829323
62-63	20.886555786193448	24.508384006859878	28.250730692209956	26.354329514736712
64-65	21.645220828518692	24.69216812979394	27.565004156710604	26.09760688497676
66-67	21.574112320071944	24.529036845710223	26.992476092378322	26.904374741839515
68-69	21.487318111232533	24.72144810386025	26.701244921754498	27.08998886315272
70-71	21.863832078909738	24.778504259985933	26.57896594364113	26.778697717463196
72-73	21.72730932724031	24.420805513000833	26.562165044939533	27.28972011481933
74-75	21.594242302242534	24.90470937011456	26.433542301719676	27.067506025923233
76	21.936451999142513	24.807980884359786	26.298122421663	26.9574446948347
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	2.0
12	4.0
13	3.0
14	3.0
15	11.5
16	25.0
17	31.0
18	49.5
19	86.5
20	129.0
21	153.0
22	207.5
23	340.5
24	497.5
25	576.0
26	715.0
27	917.0
28	1196.5
29	1413.0
30	1646.5
31	2109.5
32	2819.5
33	3300.0
34	3587.5
35	4436.5
36	5430.5
37	5863.0
38	6387.5
39	7715.0
40	9028.0
41	10047.5
42	10557.0
43	10910.0
44	11230.5
45	11093.0
46	10988.0
47	10901.5
48	10196.5
49	9323.5
50	9069.0
51	8451.0
52	7146.5
53	5951.0
54	5442.0
55	5138.5
56	4490.5
57	3819.5
58	3493.0
59	3239.0
60	2856.5
61	2625.0
62	2522.0
63	2413.5
64	2307.5
65	2275.5
66	2209.0
67	2177.0
68	2087.5
69	1930.5
70	1899.0
71	1935.0
72	1845.5
73	1723.0
74	1622.0
75	1554.0
76	1433.5
77	1198.5
78	973.5
79	863.0
80	772.5
81	587.0
82	429.0
83	366.0
84	303.5
85	192.5
86	121.0
87	98.0
88	79.0
89	43.0
90	21.5
91	11.5
92	6.0
93	6.0
94	3.5
95	1.0
96	1.0
97	0.5
98	0.5
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	2.614283398777561E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	2.614283398777561E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	2.614283398777561E-4
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	191257.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.261840262529525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.93253477059791	37.94668030875975
2	15.949806825031699	17.309317403161465
3	6.176089723572629	10.053779820826444
4	2.489547033011967	5.4034961372539865
5	1.2691497709744586	3.4433201070921
6	0.8437642986435732	2.7470522155333703
7	0.6051134264197302	2.2984197659569516
8	0.4693123658528817	2.037260210331111
9	0.3524835409175496	1.7213765033194977
>10	1.8992172647246044	16.590666824607545
>50	0.01298098025298455	0.44863070315775083
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.228566797555122E-4	0.0
5	0.0	0.0	0.0	5.228566797555122E-4	0.0
6	0.0	0.0	0.0	5.228566797555122E-4	0.0
7	0.0	0.0	0.0	5.228566797555122E-4	0.0
8	0.0	0.0	0.0	5.228566797555122E-4	0.0
9	0.0	0.0	0.0	0.0010457133595110244	0.0
10	0.0	0.0	0.0	0.0010457133595110244	0.0
11	0.0	0.0	0.0	0.0010457133595110244	0.0
12	0.0	0.0	0.0	0.0010457133595110244	0.0
13	0.0	0.0	0.0	0.0010457133595110244	0.0
14	0.0	0.0	0.0	0.0015685700392665367	0.0
15	0.0	0.0	0.0	0.0015685700392665367	0.0
16	0.0	0.0	0.0	0.002091426719022049	0.0
17	0.0	0.0	0.0	0.002091426719022049	0.0
18	0.0	0.0	0.0	0.002614283398777561	0.0
19	0.0	0.0	0.0	0.0031371400785330733	0.0
20	0.0	0.0	0.0	0.0031371400785330733	0.0
21	0.0	0.0	0.0	0.0036599967582885856	0.0
22	0.0	0.0	0.0	0.008888563555843708	0.0
23	0.0	0.0	0.0	0.00941142023559922	0.0
24	0.0	0.0	0.0	0.01202570363437678	0.0
25	0.0	0.0	0.0	0.015162843712909855	0.0
26	0.0	0.0	0.0	0.018299983791442927	0.0
27	0.0	0.0	0.0	0.028757117386553174	0.0
28	0.0	0.0	0.0	0.04757995785775161	0.0
29	0.0	0.0	0.0	0.05908280481237288	0.0
30	0.0	0.0	0.0	0.08208849872161542	0.0
31	0.0	0.0	0.0	0.10666276267012449	0.0
32	0.0	0.0	0.0	0.13123702661863357	0.0
33	0.0	0.0	0.0	0.17097413428005248	0.0
34	0.0	0.0	0.0	0.24521978280533524	0.0
35	0.0	0.0	0.0	0.34403969527912703	0.0
36	0.0	0.0	0.0	0.49305384900944804	0.0
37	0.0	0.0	0.0	0.7722593159988915	0.0
38	0.0	0.0	0.0	1.0713333368190445	0.0
39	0.0	0.0	0.0	1.4932786773817428	0.0
40	0.0	0.0	0.0	1.907904024427864	0.0
41	0.0	0.0	0.0	2.419257857228755	5.228566797555122E-4
42	0.0	0.0	0.0	2.97557736448862	5.228566797555122E-4
43	0.0	0.0	0.0	3.6631338983671187	5.228566797555122E-4
44	0.0	0.0	0.0	4.452647484797942	5.228566797555122E-4
45	0.0	0.0	0.0	5.311178152956493	5.228566797555122E-4
46	0.0	0.0	0.0	6.165003110997245	5.228566797555122E-4
47	0.0	0.0	0.0	6.990593808331198	5.228566797555122E-4
48	0.0	0.0	0.0	7.828733065979284	5.228566797555122E-4
49	0.0	0.0	0.0	8.614063798972063	5.228566797555122E-4
50	0.0	0.0	0.0	9.30318890288983	5.228566797555122E-4
51	0.0	0.0	0.0	9.996496860245639	5.228566797555122E-4
52	0.0	0.0	0.0	10.744704768975776	5.228566797555122E-4
53	0.0	0.0	0.0	11.469384127116916	5.228566797555122E-4
54	0.0	0.0	0.0	12.251577720031163	5.228566797555122E-4
55	0.0	0.0	0.0	13.081874127482916	5.228566797555122E-4
56	0.0	0.0	0.0	13.863544863717406	5.228566797555122E-4
57	0.0	0.0	0.0	14.603909922251212	5.228566797555122E-4
58	0.0	0.0	0.0	15.319700716836508	5.228566797555122E-4
59	0.0	0.0	0.0	16.025034377826692	5.228566797555122E-4
60	0.0	0.0	0.0	16.721479475261035	5.228566797555122E-4
61	0.0	0.0	0.0	17.38969031198858	5.228566797555122E-4
62	0.0	0.0	0.0	18.068358282311237	5.228566797555122E-4
63	0.0	0.0	0.0	18.75539195950998	5.228566797555122E-4
64	0.0	0.0	0.0	19.436151356551655	5.228566797555122E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTGC	20	0.0069432235	52.5	62
GTGTTAC	35	0.0012531462	40.0	1
TAGACAC	40	0.0024144112	35.0	5
TGTATAG	40	0.0024144112	35.0	3
GAATAGA	40	0.0024144112	35.0	1
GCATGTA	40	0.0024144112	35.0	2
GGTCCTA	55	2.9841997E-4	31.81818	1
TCCTGAC	45	0.004299574	31.111109	2
TAAGCAC	45	0.004299574	31.111109	20
TTAATGC	60	4.9689563E-4	29.166666	56
GCCTTAC	50	0.0071955845	27.999998	1
TAGACTG	65	7.9349696E-4	26.923077	5
TATAGGA	65	7.9349696E-4	26.923077	5
TTGGACA	70	0.0012228186	25.0	26
ACCCATC	70	0.0012228186	25.0	66
ATTAGGC	105	2.0291285E-5	23.333332	3
GGTCTAG	95	2.7696794E-4	22.105265	1
ACGCCCG	80	0.0026591043	21.875	5
GCTGCTC	80	0.0026591043	21.875	8
CTAGGAC	80	0.0026591043	21.875	3
>>END_MODULE