FastQCFastQC Report
Wed 25 May 2016
SRR1781199_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781199_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178699
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT36122.021276000425296No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG3720.20817128243582786No Hit
CTTATACACATCTCCGAGCCCACGAGACTACACCTCACCTCGTATGCCGT2350.13150605207639662No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC2170.12143324808756625No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5600.056.87543
TCGTATG5500.056.63636440
ATCTCGT4600.056.30434437
TCTCGTA4600.056.30434438
CTCGTAT5350.056.26168439
CGTATGC5600.056.2541
CCGTCTT5400.055.0925947
GCCGTCT5500.054.72727246
CATCTCG4750.054.52631836
TGCCGTC5850.053.24786445
GTATGCC6000.053.08333242
CGTCTTC5500.052.81818448
ATGCCGT5850.052.64957444
CCGCGAT200.00694247752.50000429
TTGAAAA5400.051.85185258
GTCTTCT5650.050.7964649
CTGCTTG5700.049.73684354
CTTGAAA5500.049.63636457
TCATCTC5300.049.528335
TCTTCTG6050.048.0165350