##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781199_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	178699
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.16175244405397	34.0	34.0	34.0	32.0	34.0
2	33.2393969748012	34.0	34.0	34.0	32.0	34.0
3	33.33166385933889	34.0	34.0	34.0	33.0	34.0
4	33.41982887425223	34.0	34.0	34.0	34.0	34.0
5	33.41753451334367	34.0	34.0	34.0	34.0	34.0
6	36.82031796484591	38.0	37.0	38.0	34.0	38.0
7	36.73244953805002	38.0	37.0	38.0	34.0	38.0
8	36.48360651150818	38.0	37.0	38.0	34.0	38.0
9	36.475895220454504	38.0	37.0	38.0	34.0	38.0
10-11	36.40795135954874	38.0	37.0	38.0	34.0	38.0
12-13	36.346862601357586	38.0	37.0	38.0	34.0	38.0
14-15	36.344778090532124	38.0	37.0	38.0	34.0	38.0
16-17	36.289047504462815	38.0	37.0	38.0	34.0	38.0
18-19	36.27222312380036	38.0	37.0	38.0	34.0	38.0
20-21	36.22404154472045	38.0	37.0	38.0	33.5	38.0
22-23	36.30375379828651	38.0	37.0	38.0	34.0	38.0
24-25	36.27331714223359	38.0	37.0	38.0	34.0	38.0
26-27	36.054267231489824	38.0	37.0	38.0	32.5	38.0
28-29	36.03372150935372	38.0	37.0	38.0	32.0	38.0
30-31	36.07798029088019	38.0	37.0	38.0	32.5	38.0
32-33	35.94898404579769	38.0	37.0	38.0	32.0	38.0
34-35	35.97273068120135	38.0	37.0	38.0	32.0	38.0
36-37	35.97465290796255	38.0	37.0	38.0	32.0	38.0
38-39	35.80466874464882	38.0	36.0	38.0	31.0	38.0
40-41	35.91208680518638	38.0	36.5	38.0	32.0	38.0
42-43	35.956608598817006	38.0	37.0	38.0	32.0	38.0
44-45	35.918975483914295	38.0	37.0	38.0	32.0	38.0
46-47	35.94183235496561	38.0	37.0	38.0	32.0	38.0
48-49	35.92513668235412	38.0	37.0	38.0	32.0	38.0
50-51	35.82056418894342	38.0	36.0	38.0	31.0	38.0
52-53	35.84526494272492	38.0	36.5	38.0	31.5	38.0
54-55	35.857576147600156	38.0	37.0	38.0	31.5	38.0
56-57	35.81297880793961	38.0	36.0	38.0	31.0	38.0
58-59	35.800426974969085	38.0	36.0	38.0	31.0	38.0
60-61	35.7674161578968	38.0	36.0	38.0	31.0	38.0
62-63	35.7483058103291	38.0	36.0	38.0	31.0	38.0
64-65	35.69629376773233	38.0	36.0	38.0	31.0	38.0
66-67	35.66732326426001	38.0	36.0	38.0	31.0	38.0
68-69	35.56518782981438	38.0	36.0	38.0	31.0	38.0
70-71	35.36789517568649	38.0	36.0	38.0	29.5	38.0
72-73	35.21504876915931	38.0	36.0	38.0	27.0	38.0
74-75	35.20231226811566	38.0	36.0	38.0	27.0	38.0
76	34.276425721464584	37.0	34.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	3.0
21	10.0
22	19.0
23	49.0
24	133.0
25	272.0
26	511.0
27	917.0
28	1662.0
29	2477.0
30	3814.0
31	5496.0
32	7971.0
33	10721.0
34	14720.0
35	20479.0
36	31029.0
37	78414.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.921336996849455	18.16630199385559	10.340292894755986	28.572068114538972
2	22.965993094533268	19.39350527982809	32.7254209592667	24.915080666371946
3	20.38119967095507	21.383443667843693	29.025344294036344	29.210012367164893
4	14.683350214606685	17.7393270247735	34.91625582683731	32.66106693378251
5	14.714128226794779	33.142882724581554	35.83232138959927	16.310667659024393
6	31.50493287595342	37.44732762914174	16.735404227220073	14.312335267684768
7	30.180918751643826	27.55023810989429	20.5250169279067	21.743826210555177
8	26.139485951236434	35.31469118461771	18.08683876238815	20.458984101757704
9	24.47243689108501	16.039261551547572	19.97325110940744	39.51505044795998
10-11	25.74216979389924	24.895214858505085	26.521692902590388	22.840922445005287
12-13	25.517210504815356	21.738230208339164	26.63109474591352	26.113464540931957
14-15	23.251669007660926	22.893524865835847	22.472425699080578	31.382380427422653
16-17	25.00321770127421	27.07122032020325	23.75027280510803	24.175289173414512
18-19	24.90360886182911	25.553864319330273	24.696556779836484	24.845970039004136
20-21	23.234321400791274	25.559180521435486	24.691240577731268	26.515257500041972
22-23	23.571480534306293	26.44250947123375	24.975517490304927	25.010492504155028
24-25	24.775460411082324	26.360528038769104	24.82106782914286	24.042943721005713
26-27	24.126883754245966	26.800653613058834	25.08659813429286	23.98586449840234
28-29	23.310427030929105	25.476079888527636	26.980285284192973	24.233207796350285
30-31	23.307069429599494	26.623819943032697	25.169978567311514	24.899132060056296
32-33	22.845399246778104	26.96629527865293	25.07036972786641	25.117935746702557
34-35	23.56868253319828	24.836456835236906	26.65487775533159	24.93998287623322
36-37	23.264260012646965	26.311283219268155	25.081002132076847	25.343454636008033
38-39	22.663249374646753	25.662706562431797	26.86081063688101	24.813233426040437
40-41	22.823574838135634	24.54882232133364	27.49819528928533	25.129407551245393
42-43	24.0051707060476	24.58547613584855	27.228188182362523	24.181164975741332
44-45	22.384848264399913	25.758118400214887	27.69237656618112	24.16465676920408
46-47	23.721173593584744	23.978309895410717	27.01442089771068	25.286095613293863
48-49	23.090224343728842	23.951449084773838	27.274914800866263	25.68341177063106
50-51	21.73767060811756	24.09834414294428	28.464345071880647	25.69964017705751
52-53	21.11455576136408	24.067006530534584	30.235479773249992	24.582957934851343
54-55	20.53732813278194	24.71054678537653	28.742186581905887	26.009938499935647
56-57	21.914224478032892	24.478032893301023	27.372844839646554	26.234897789019524
58-59	20.565028343751223	24.395212060503976	29.522269290818638	25.51749030492616
60-61	21.97354210152267	25.64535895556215	26.828633624138913	25.552465318776267
62-63	19.850139060655067	27.325558620921214	26.606752136273847	26.217550182149875
64-65	20.411977683143164	27.367528637541337	25.953698677664676	26.266795001650824
66-67	20.485285312172984	27.44139586679276	25.20467378105082	26.868645039983434
68-69	20.34174785533215	27.555834112110304	25.07008992775561	27.032328104801927
70-71	20.405262480483945	27.31408681637838	25.193481776618782	27.087168926518896
72-73	20.50095411837783	25.87087784486763	25.25867520243538	28.36949283431916
74-75	20.43799909344764	26.224824985030693	25.08939613540087	28.247779786120798
76	20.671072585744742	26.082966328854667	24.939143475900817	28.306817609499774
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	1.0
12	1.0
13	2.0
14	4.5
15	8.5
16	14.0
17	17.0
18	29.0
19	71.5
20	129.0
21	156.0
22	181.0
23	280.5
24	448.0
25	541.0
26	632.0
27	855.0
28	1161.5
29	1336.0
30	1547.0
31	1908.5
32	2503.5
33	2948.0
34	3229.5
35	4068.0
36	5019.5
37	5414.0
38	5792.5
39	6791.5
40	8260.0
41	9775.0
42	10442.0
43	10971.0
44	11358.0
45	10971.5
46	10727.0
47	10375.0
48	9490.0
49	8644.0
50	8331.0
51	7716.0
52	6369.0
53	5302.5
54	4968.0
55	4618.5
56	3995.0
57	3387.5
58	3054.0
59	2829.5
60	2548.5
61	2397.5
62	2303.0
63	2283.0
64	2188.0
65	2060.5
66	2052.5
67	2097.0
68	2033.5
69	1914.5
70	1774.0
71	1689.0
72	1667.0
73	1613.0
74	1509.5
75	1438.0
76	1339.0
77	1127.5
78	952.5
79	890.0
80	773.5
81	551.0
82	394.5
83	344.0
84	296.0
85	191.5
86	116.0
87	97.0
88	88.5
89	50.5
90	18.0
91	10.0
92	5.0
93	4.5
94	3.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	178699.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.29908953043945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.28718810264662	29.461272866664057
2	20.047797640878816	18.9648515100812
3	7.771849082498256	11.02804156710446
4	3.2689327165386937	6.184701649141853
5	1.547507778947742	3.659785449275038
6	0.9796150160311394	2.780093900917185
7	0.7642890100919276	2.530512202082832
8	0.5371319049252866	2.03246800485733
9	0.4614128698697396	1.9641967778219238
>10	2.3023319096577266	18.035355542000794
>50	0.024845308377601363	0.75266229805427
>100	0.00591554961371461	0.5847822315737637
>500	0.0	0.0
>1k	0.0011831099227429221	2.021276000425296
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT	3612	2.021276000425296	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG	372	0.20817128243582786	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACACCTCACCTCGTATGCCGT	235	0.13150605207639662	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC	217	0.12143324808756625	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.596002216016878E-4	0.0
5	0.0	0.0	0.0	5.596002216016878E-4	0.0
6	0.0	0.0	0.0	5.596002216016878E-4	0.0
7	0.0	0.0	0.0	5.596002216016878E-4	0.0
8	0.0	0.0	0.0	5.596002216016878E-4	0.0
9	0.0	0.0	0.0	0.0011192004432033756	0.0
10	0.0	0.0	0.0	0.0011192004432033756	0.0
11	0.0	0.0	0.0	0.0011192004432033756	0.0
12	0.0	0.0	0.0	0.0016788006648050633	0.0
13	0.0	0.0	0.0	0.002238400886406751	0.0
14	0.0	0.0	0.0	0.0027980011080084386	0.0
15	0.0	0.0	0.0	0.0027980011080084386	0.0
16	0.0	0.0	0.0	0.003917201551211814	0.0
17	0.0	0.0	0.0	0.003917201551211814	0.0
18	0.0	0.0	0.0	0.004476801772813502	0.0
19	0.0	0.0	0.0	0.00503640199441519	0.0
20	0.0	0.0	0.0	0.005596002216016877	0.0
21	0.0	0.0	0.0	0.006155602437618565	0.0
22	0.0	0.0	0.0	0.006715202659220253	0.0
23	0.0	0.0	0.0	0.008394003324025316	0.0
24	0.0	0.0	0.0	0.009513203767228692	0.0
25	0.0	0.0	0.0	0.011751604653635443	0.0
26	0.0	0.0	0.0	0.015668806204847258	0.0
27	0.0	0.0	0.0	0.02518200997207595	0.0
28	0.0	0.0	0.0	0.04364881728493165	0.0
29	0.0	0.0	0.0	0.05763882282497384	0.0
30	0.0	0.0	0.0	0.08114203213224472	0.0
31	0.0	0.0	0.0	0.10744324254752405	0.0
32	0.0	0.0	0.0	0.13542325362760843	0.0
33	0.0	0.0	0.0	0.18187007202054853	0.0
34	0.0	0.0	0.0	0.2602141030447848	0.0
35	0.0	0.0	0.0	0.3654189447059021	0.0
36	0.0	0.0	0.0	0.5277030089703916	0.0
37	0.0	0.0	0.0	0.8259699270840911	0.0
38	0.0	0.0	0.0	1.1427036525106464	0.0
39	0.0	0.0	0.0	1.5954202317864117	0.0
40	0.0	0.0	0.0	2.023514401311703	0.0
41	0.0	0.0	0.0	2.5456214080660775	0.0
42	0.0	0.0	0.0	3.105781229889367	0.0
43	0.0	0.0	0.0	3.810317908885892	0.0
44	0.0	5.596002216016878E-4	0.0	4.620619029765136	0.0
45	0.0	5.596002216016878E-4	0.0	5.489118573690956	0.0
46	0.0	5.596002216016878E-4	0.0	6.357058517395173	0.0
47	0.0	5.596002216016878E-4	0.0	7.207091254008136	0.0
48	0.0	5.596002216016878E-4	0.0	8.074471597490753	0.0
49	0.0	5.596002216016878E-4	0.0	8.87917671615398	0.0
50	0.0	5.596002216016878E-4	0.0	9.599382201355352	0.0
51	0.0	5.596002216016878E-4	0.0	10.293286476141445	0.0
52	0.0	5.596002216016878E-4	0.0	11.050425575968529	0.0
53	0.0	5.596002216016878E-4	0.0	11.785740267153146	0.0
54	0.0	5.596002216016878E-4	0.0	12.580372581827543	0.0
55	0.0	5.596002216016878E-4	0.0	13.402984907582024	0.0
56	0.0	5.596002216016878E-4	0.0	14.20713042602365	0.0
57	0.0	5.596002216016878E-4	0.0	14.996166738482028	0.0
58	0.0	5.596002216016878E-4	0.0	15.731481429666646	0.0
59	0.0	5.596002216016878E-4	0.0	16.470713322402474	0.0
60	0.0	5.596002216016878E-4	0.0	17.192038008047053	0.0
61	0.0	5.596002216016878E-4	0.0	17.870273476628295	0.0
62	0.0	5.596002216016878E-4	0.0	18.55746254875517	0.0
63	0.0	5.596002216016878E-4	0.0	19.261999227751694	0.0
64	0.0	5.596002216016878E-4	0.0	19.959261103867398	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	560	0.0	56.875	43
TCGTATG	550	0.0	56.636364	40
ATCTCGT	460	0.0	56.304344	37
TCTCGTA	460	0.0	56.304344	38
CTCGTAT	535	0.0	56.261684	39
CGTATGC	560	0.0	56.25	41
CCGTCTT	540	0.0	55.09259	47
GCCGTCT	550	0.0	54.727272	46
CATCTCG	475	0.0	54.526318	36
TGCCGTC	585	0.0	53.247864	45
GTATGCC	600	0.0	53.083332	42
CGTCTTC	550	0.0	52.818184	48
ATGCCGT	585	0.0	52.649574	44
CCGCGAT	20	0.006942477	52.500004	29
TTGAAAA	540	0.0	51.851852	58
GTCTTCT	565	0.0	50.79646	49
CTGCTTG	570	0.0	49.736843	54
CTTGAAA	550	0.0	49.636364	57
TCATCTC	530	0.0	49.5283	35
TCTTCTG	605	0.0	48.01653	50
>>END_MODULE