FastQCFastQC Report
Wed 25 May 2016
SRR1781198_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781198_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247234
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGTA252.3873545E-456.00000433
GTCGCAG200.006945616652.528
TTTCGGA200.006945616652.525
CGAATTG200.006945616652.547
CTCTTGA404.5842964E-543.752
CTATCGT350.001253857640.00000432
ATTTCGG350.001253857640.00000448
CAGTCGC459.180954E-538.8888944
GTACCTA400.002415774235.01
AATTTCG400.002415774235.047
ACGTTTA400.002415774235.026
CGACCCT400.002415774235.010
GGTCTAT400.002415774235.01
CTTACGT400.002415774235.023
AATGCGG450.004301985331.11111342
GCTGTCG450.004301985331.11111354
AGGTGAC450.004301985331.1111137
GAATGCG500.007199595728.00000241
ATCGGTT500.007199595728.00000225
GACTATC500.007199595728.00000238