##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781198_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	247234
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.809625698730756	34.0	32.0	34.0	21.0	34.0
2	30.98044362830355	34.0	32.0	34.0	21.0	34.0
3	31.03479699394096	34.0	32.0	34.0	23.0	34.0
4	31.137189868707377	34.0	32.0	34.0	23.0	34.0
5	31.160584709222842	34.0	32.0	34.0	23.0	34.0
6	33.951014019107404	37.0	34.0	38.0	23.0	38.0
7	33.92833509954133	37.0	34.0	38.0	24.0	38.0
8	33.70638747097891	37.0	34.0	38.0	23.0	38.0
9	34.00605094768519	37.0	34.0	38.0	24.0	38.0
10-11	33.83176262164589	37.0	34.0	38.0	23.0	38.0
12-13	33.80865293608484	37.0	34.0	38.0	23.0	38.0
14-15	33.66233406408503	37.0	34.0	38.0	22.0	38.0
16-17	33.618141113277304	37.0	34.0	38.0	22.0	38.0
18-19	33.670929160228766	37.0	34.0	38.0	22.0	38.0
20-21	33.62032325651003	37.0	34.0	38.0	22.0	38.0
22-23	33.669129245977494	37.0	34.0	38.0	22.5	38.0
24-25	33.663602093563185	37.0	34.0	38.0	22.5	38.0
26-27	33.5965138290041	37.0	34.0	38.0	22.0	38.0
28-29	33.67761715621638	37.0	34.0	38.0	22.5	38.0
30-31	33.75346837409094	37.0	34.0	38.0	23.0	38.0
32-33	33.6953655241593	37.0	34.0	38.0	22.5	38.0
34-35	33.74162332041709	37.0	34.0	38.0	22.5	38.0
36-37	33.582341021056976	37.0	34.0	38.0	22.0	38.0
38-39	33.59626305443426	37.0	34.0	38.0	22.0	38.0
40-41	33.57538404911946	37.0	34.0	38.0	22.0	38.0
42-43	33.66215609503547	37.0	34.0	38.0	22.5	38.0
44-45	33.674395511944155	37.0	34.0	38.0	22.5	38.0
46-47	33.654230405203165	37.0	34.0	38.0	22.5	38.0
48-49	33.62207665612335	37.0	34.0	38.0	22.0	38.0
50-51	33.483325513481155	37.0	34.0	38.0	22.0	38.0
52-53	33.56069351302814	37.0	34.0	38.0	22.0	38.0
54-55	33.53083516021259	37.0	34.0	38.0	22.0	38.0
56-57	33.520694969138546	37.0	34.0	38.0	22.0	38.0
58-59	33.510661963969355	37.0	34.0	38.0	22.0	38.0
60-61	33.48364504882015	37.0	34.0	38.0	22.0	38.0
62-63	33.476030804824575	37.0	34.0	38.0	22.0	38.0
64-65	33.469565270148934	37.0	34.0	38.0	22.0	38.0
66-67	33.36765574314212	37.0	34.0	38.0	21.0	38.0
68-69	33.1516215407266	37.0	33.5	38.0	21.0	38.0
70-71	33.293125136510355	37.0	34.0	38.0	21.0	38.0
72-73	33.25091006900345	37.0	33.0	38.0	21.0	38.0
74-75	33.22712491000429	37.0	33.0	38.0	21.0	38.0
76	31.888397226918627	36.0	30.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	8.0
13	37.0
14	96.0
15	331.0
16	675.0
17	1435.0
18	1986.0
19	2305.0
20	2423.0
21	2571.0
22	2774.0
23	3209.0
24	3644.0
25	4214.0
26	4974.0
27	5708.0
28	6545.0
29	7791.0
30	9030.0
31	10562.0
32	12596.0
33	14844.0
34	18166.0
35	23244.0
36	34960.0
37	73106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.09101498984767	17.927145942710148	9.069140975755762	25.91269809168642
2	23.643188234627925	19.98996901720637	31.58384364610046	24.78299910206525
3	20.042146306737745	21.71384194730498	29.61526327285082	28.62874847310645
4	14.045398286643424	16.1628255013469	36.37687373095934	33.41490248105034
5	14.19303170275933	33.050874879668655	36.125290210893326	16.630803206678692
6	30.33846477426244	37.07580672561217	18.10147471626071	14.484253783864679
7	29.38188113285389	29.085400875284144	21.823859177944783	19.70885881391718
8	26.246390059619635	34.032940453173914	19.58144915343359	20.139220333772865
9	25.119926870899633	15.954520818334048	21.18559745018889	37.73995486057743
10-11	25.468786655557086	24.11723306664941	27.690366211766992	22.723614066026517
12-13	25.516312481293024	22.48760283779739	26.861798943511005	25.134285737398578
14-15	23.512138298130516	23.826213223100382	24.10894132684016	28.552707151928942
16-17	23.76008154218271	26.850271402800587	25.128623085821527	24.261023969195175
18-19	23.155593486332787	26.39159662505966	26.623563102162322	23.82924678644523
20-21	23.251049612917317	26.541252416738796	26.49858029235461	23.709117677989273
22-23	23.633480831924413	26.308679226967165	26.515972722198406	23.54186721891002
24-25	23.50789130944773	26.083184351666844	26.28663533332794	24.122289005557487
26-27	22.785498758261404	26.350340163569737	26.747130249075774	24.117030829093085
28-29	23.077529789592045	26.42112330828284	27.293171651148306	23.208175250976808
30-31	23.177232904859363	26.40635996667125	26.83287897295679	23.583528155512592
32-33	22.84273198670086	26.56895896195507	26.72124384186641	23.867065209477662
34-35	23.805180517242775	25.909462290785246	26.849462452575295	23.435894739396684
36-37	23.69354538615239	25.769716139365944	26.30524118850967	24.231497285971994
38-39	22.69509857058495	26.421932258508136	27.3402930017716	23.542676169135312
40-41	23.165503126592622	25.49487530032277	27.744768114417923	23.594853458666687
42-43	22.878528034170056	25.868812541964292	27.186390221409678	24.066269202455974
44-45	22.368889392235697	25.486179085400874	27.714836956082088	24.430094566281337
46-47	22.549285292475954	25.267155811902892	27.898266419667202	24.285292475953955
48-49	21.986053698115953	25.290210893323735	28.39718647111643	24.326548937443878
50-51	22.08292548759475	25.238235841348683	28.158546154655106	24.520292516401465
52-53	21.684113026525477	25.019414805407024	28.728047113261123	24.56842505480638
54-55	21.09782635074464	25.707831447131056	28.54037066099323	24.653971541131074
56-57	21.163351318993342	25.57051214638763	28.460284588689255	24.805851945929767
58-59	21.170429633464654	25.498920051449236	28.318516061706724	25.012134253379394
60-61	20.833704102186594	25.555951042332364	28.54562883745763	25.064716018023407
62-63	20.692340050316705	25.3955766601681	28.442689921289144	25.46939336822605
64-65	21.425855667100805	25.28859299287315	27.950039234085928	25.335512105940122
66-67	21.343342744120957	25.19192344095068	27.598145886083632	25.866587928844737
68-69	21.116027730813723	25.373330528972556	27.217534805083442	26.293106935130282
70-71	21.656608718865527	25.279896777951254	27.102259397979246	25.96123510520398
72-73	21.323523463601283	24.927194479723664	27.33200126196235	26.4172807947127
74-75	21.479650857082763	25.08493977365573	27.127943567632286	26.307465801629228
76	21.527783395487678	25.17655338667012	26.584126778679305	26.7115364391629
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	0.5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.5
11	3.5
12	5.0
13	7.5
14	16.0
15	27.5
16	43.5
17	54.0
18	85.5
19	158.5
20	269.5
21	339.0
22	382.0
23	588.0
24	855.0
25	959.0
26	1200.0
27	1511.0
28	1856.5
29	2132.0
30	2414.0
31	3045.0
32	3963.0
33	4532.0
34	5016.0
35	6146.5
36	7557.0
37	8321.0
38	9027.0
39	10498.5
40	12066.5
41	13354.0
42	13839.0
43	13959.0
44	14233.0
45	14408.0
46	14429.0
47	14207.0
48	13258.0
49	12067.5
50	11604.0
51	10931.0
52	9257.0
53	7764.0
54	7272.0
55	6888.0
56	6003.5
57	5187.0
58	4871.0
59	4428.0
60	3705.5
61	3289.5
62	3153.0
63	3023.0
64	2748.0
65	2467.0
66	2275.0
67	2219.0
68	2149.5
69	2063.5
70	1977.5
71	1908.0
72	1866.5
73	1761.5
74	1585.5
75	1473.0
76	1338.0
77	1093.5
78	899.5
79	815.0
80	718.5
81	535.5
82	376.0
83	303.0
84	256.0
85	173.0
86	109.5
87	82.0
88	64.5
89	33.5
90	16.0
91	8.0
92	4.0
93	4.0
94	3.5
95	2.0
96	1.0
97	0.5
98	1.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	247234.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.020811444842224
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.31292536897432	41.070495685651
2	13.950352632794027	15.630201488616247
3	5.560860647961585	9.345717774914972
4	2.3889505239488416	5.353237874127803
5	1.1615685348568998	3.2536005935740007
6	0.6783084214971697	2.2799632909284906
7	0.47792596162340006	1.8741660126489577
8	0.38593577985604727	1.729634844250701
9	0.29266025546181934	1.475555848976334
>10	1.7688350923219707	17.214289881951085
>50	0.021676780703913698	0.7731367043604079
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.044751126463189E-4	0.0
3	0.0	0.0	0.0	4.044751126463189E-4	0.0
4	0.0	0.0	0.0	0.0016179004505852755	0.0
5	0.0	0.0	0.0	0.0016179004505852755	0.0
6	0.0	0.0	0.0	0.0016179004505852755	0.0
7	0.0	0.0	0.0	0.0016179004505852755	0.0
8	0.0	0.0	0.0	0.0016179004505852755	4.044751126463189E-4
9	0.0	0.0	0.0	0.002426850675877913	4.044751126463189E-4
10	0.0	0.0	0.0	0.002831325788524232	4.044751126463189E-4
11	0.0	0.0	0.0	0.003235800901170551	4.044751126463189E-4
12	0.0	0.0	0.0	0.003235800901170551	4.044751126463189E-4
13	0.0	0.0	0.0	0.004449226239109508	4.044751126463189E-4
14	0.0	0.0	0.0	0.004449226239109508	8.089502252926377E-4
15	0.0	0.0	0.0	0.004449226239109508	8.089502252926377E-4
16	0.0	0.0	0.0	0.004449226239109508	8.089502252926377E-4
17	0.0	0.0	0.0	0.004449226239109508	8.089502252926377E-4
18	0.0	0.0	0.0	0.004853701351755826	8.089502252926377E-4
19	0.0	0.0	0.0	0.005258176464402145	8.089502252926377E-4
20	0.0	0.0	0.0	0.005662651577048464	8.089502252926377E-4
21	0.0	0.0	0.0	0.006067126689694783	0.0012134253379389565
22	0.0	0.0	0.0	0.009707402703511652	0.0012134253379389565
23	0.0	0.0	0.0	0.011325303154096928	0.0012134253379389565
24	0.0	0.0	0.0	0.012943203604682204	0.0012134253379389565
25	0.0	0.0	0.0	0.01456110405526748	0.0012134253379389565
26	0.0	0.0	0.0	0.016583479618499073	0.0012134253379389565
27	0.0	0.0	0.0	0.026695357434657046	0.0012134253379389565
28	0.0	0.0	0.0	0.03518933480022974	0.0012134253379389565
29	0.0	0.0	0.0	0.04691911306697299	0.0012134253379389565
30	0.0	0.0	0.0	0.06067126689694783	0.0012134253379389565
31	0.0	0.0	0.0	0.07401894561427635	0.0012134253379389565
32	0.0	0.0	0.0	0.0942427012465923	0.0012134253379389565
33	0.0	0.0	0.0	0.13347678717328523	0.0012134253379389565
34	0.0	0.0	0.0	0.19212567850700146	0.0012134253379389565
35	0.0	0.0	0.0	0.2677625245718631	0.0012134253379389565
36	0.0	0.0	0.0	0.39881246106927043	0.0012134253379389565
37	0.0	0.0	0.0	0.6192513974615141	0.0012134253379389565
38	0.0	0.0	0.0	0.8720483428654635	0.0012134253379389565
39	0.0	0.0	0.0	1.179449428476666	0.0012134253379389565
40	0.0	0.0	0.0	1.5236577493386831	0.0012134253379389565
41	0.0	0.0	0.0	1.8804047986927364	0.0012134253379389565
42	0.0	0.0	0.0	2.2771948841987752	0.0012134253379389565
43	0.0	0.0	0.0	2.7520486664455537	0.0012134253379389565
44	0.0	0.0	0.0	3.3037527200951327	0.0012134253379389565
45	0.0	0.0	0.0	3.9796306333271314	0.0012134253379389565
46	0.0	0.0	0.0	4.721842465033126	0.0012134253379389565
47	0.0	0.0	0.0	5.4648632469644145	0.0012134253379389565
48	0.0	0.0	0.0	6.208288504008348	0.0012134253379389565
49	0.0	0.0	0.0	6.929872104969381	0.0012134253379389565
50	0.0	0.0	0.0	7.578650185654077	0.0012134253379389565
51	0.0	0.0	0.0	8.205182135143225	0.0012134253379389565
52	0.0	0.0	0.0	8.81432165478858	0.0012134253379389565
53	0.0	0.0	0.0	9.441258079390375	0.0012134253379389565
54	0.0	0.0	0.0	10.064958703091	0.0012134253379389565
55	0.0	0.0	0.0	10.785733353826739	0.0012134253379389565
56	0.0	0.0	0.0	11.515001981928052	0.0012134253379389565
57	0.0	0.0	0.0	12.20099177297621	0.0012134253379389565
58	0.0	0.0	0.0	12.844107202083856	0.0012134253379389565
59	0.0	0.0	0.0	13.531714893582597	0.0012134253379389565
60	0.0	0.0	0.0	14.172403472014366	0.0012134253379389565
61	0.0	0.0	0.0	14.754847634225067	0.0012134253379389565
62	0.0	0.0	0.0	15.336887321323118	0.0012134253379389565
63	0.0	0.0	0.0	15.938341813828195	0.0012134253379389565
64	0.0	0.0	0.0	16.506224871983626	0.0012134253379389565
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGTA	25	2.3873545E-4	56.000004	33
GTCGCAG	20	0.0069456166	52.5	28
TTTCGGA	20	0.0069456166	52.5	25
CGAATTG	20	0.0069456166	52.5	47
CTCTTGA	40	4.5842964E-5	43.75	2
CTATCGT	35	0.0012538576	40.000004	32
ATTTCGG	35	0.0012538576	40.000004	48
CAGTCGC	45	9.180954E-5	38.88889	44
GTACCTA	40	0.0024157742	35.0	1
AATTTCG	40	0.0024157742	35.0	47
ACGTTTA	40	0.0024157742	35.0	26
CGACCCT	40	0.0024157742	35.0	10
GGTCTAT	40	0.0024157742	35.0	1
CTTACGT	40	0.0024157742	35.0	23
AATGCGG	45	0.0043019853	31.111113	42
GCTGTCG	45	0.0043019853	31.111113	54
AGGTGAC	45	0.0043019853	31.111113	7
GAATGCG	50	0.0071995957	28.000002	41
ATCGGTT	50	0.0071995957	28.000002	25
GACTATC	50	0.0071995957	28.000002	38
>>END_MODULE