FastQCFastQC Report
Wed 25 May 2016
SRR1781198_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781198_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221588
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT35821.616513529613517No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG4190.18908966189504847No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGACCTCGTATGCCGT2850.12861707312670362No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC2480.11191941801902631No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAC150.00222252870.0000134
GAGACGA207.9145204E-570.053
CGAGAAT207.9145204E-570.014
ACTCGTA352.9649345E-760.038
TCCGGAT200.006944669452.53
GTCTATA200.006944669452.51
AGCGCAC200.006944669452.524
ACGGTAA200.006944669452.513
TATGCCG6300.051.66666443
CGTATGC6350.051.25984241
TCGTATG6400.050.85937540
CTCGTAT6200.050.24193639
TCTCGTA5550.049.81981738
GTATGCC6500.049.53846442
TGCCGTC6500.049.53846445
ATGCCGT6550.049.1603144
CCGTCTT6350.049.0551247
GCCGTCT6450.048.8372146
ATCTCGT5600.048.7537
TCGCAGA6850.048.02919831
Produced by FastQC (version 0.11.5)