##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781198_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	221588
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.3179865335668	34.0	34.0	34.0	33.0	34.0
2	33.32937252919833	34.0	34.0	34.0	33.0	34.0
3	33.39498528801199	34.0	34.0	34.0	34.0	34.0
4	33.45934346625268	34.0	34.0	34.0	34.0	34.0
5	33.45833258118671	34.0	34.0	34.0	34.0	34.0
6	36.90614563965558	38.0	37.0	38.0	35.0	38.0
7	36.80307146596386	38.0	37.0	38.0	34.0	38.0
8	36.588628445583694	38.0	37.0	38.0	34.0	38.0
9	36.55808076249616	38.0	37.0	38.0	34.0	38.0
10-11	36.48868395400473	38.0	37.0	38.0	34.0	38.0
12-13	36.43435565102803	38.0	37.0	38.0	34.0	38.0
14-15	36.43960187374768	38.0	37.0	38.0	34.0	38.0
16-17	36.374792407531096	38.0	37.0	38.0	34.0	38.0
18-19	36.3587874794664	38.0	37.0	38.0	34.0	38.0
20-21	36.31856869505569	38.0	37.0	38.0	34.0	38.0
22-23	36.38065012545806	38.0	37.0	38.0	34.0	38.0
24-25	36.36790124013936	38.0	37.0	38.0	34.0	38.0
26-27	36.16309773092405	38.0	37.0	38.0	33.5	38.0
28-29	36.138376175605174	38.0	37.0	38.0	34.0	38.0
30-31	36.17804664512519	38.0	37.0	38.0	34.0	38.0
32-33	36.13862889687167	38.0	37.0	38.0	33.5	38.0
34-35	36.122554019170714	38.0	37.0	38.0	33.5	38.0
36-37	36.0775019405383	38.0	37.0	38.0	32.5	38.0
38-39	35.917522609527595	38.0	37.0	38.0	32.0	38.0
40-41	36.01765664205643	38.0	37.0	38.0	32.0	38.0
42-43	36.075349296893336	38.0	37.0	38.0	32.5	38.0
44-45	36.05844630575663	38.0	37.0	38.0	32.5	38.0
46-47	36.080922703395494	38.0	37.0	38.0	33.0	38.0
48-49	36.05787768290702	38.0	37.0	38.0	32.5	38.0
50-51	35.9468337635612	38.0	37.0	38.0	32.0	38.0
52-53	35.96705597776053	38.0	37.0	38.0	32.0	38.0
54-55	35.98332265285124	38.0	37.0	38.0	32.0	38.0
56-57	35.959616495478095	38.0	37.0	38.0	32.0	38.0
58-59	35.932151109265845	38.0	37.0	38.0	32.0	38.0
60-61	35.94074363232666	38.0	37.0	38.0	32.0	38.0
62-63	35.90608471577883	38.0	37.0	38.0	32.0	38.0
64-65	35.87680740834341	38.0	37.0	38.0	32.0	38.0
66-67	35.83610348935863	38.0	37.0	38.0	31.0	38.0
68-69	35.74192645811145	38.0	36.5	38.0	31.0	38.0
70-71	35.584167463942094	38.0	36.0	38.0	31.0	38.0
72-73	35.442648067584884	38.0	36.0	38.0	31.0	38.0
74-75	35.469244724443556	38.0	36.0	38.0	31.0	38.0
76	34.592378648663285	37.0	35.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	9.0
22	20.0
23	62.0
24	147.0
25	310.0
26	559.0
27	1078.0
28	1767.0
29	2844.0
30	4304.0
31	6264.0
32	9152.0
33	12647.0
34	17137.0
35	24255.0
36	36641.0
37	104389.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.36572377565572	18.02940592450855	9.148961135079517	27.455909164756214
2	23.21966893514089	20.313374370453275	31.80000722060761	24.66694947379822
3	19.93293860678376	21.803527266819504	30.547231799555934	27.716302326840804
4	14.069353936133725	17.860624221528244	35.909435528999765	32.16058631333827
5	13.617614672274673	33.777099842951785	36.72807191725184	15.877213567521705
6	30.196581042294707	38.6677076375977	17.286134628228965	13.849576691878621
7	28.63377078181129	29.134700435041612	21.342310955466903	20.8892178276802
8	25.257234146253403	36.06964276043829	18.92792028449194	19.745202808816362
9	24.57037384695922	15.822607722439844	20.288102243803817	39.31891618679712
10-11	24.906583388992185	25.22925429174865	27.64071159088037	22.223450728378793
12-13	24.962543098001696	22.563721862194704	26.891799194902248	25.58193584490135
14-15	22.847130711049335	23.377845370687943	23.412143256855064	30.362880661407658
16-17	24.427315558604256	27.55519251945051	24.257405635684243	23.76008628626099
18-19	23.94556564434897	26.386807949888986	25.350425113273285	24.317201292488765
20-21	22.848710218964925	26.254806216943155	25.260393162084586	25.636090402007326
22-23	22.865633518060545	26.820495694712708	25.7775691824467	24.536301604780043
24-25	24.072828853548025	27.024703503799845	25.475657526580864	23.426810116071266
26-27	23.152201382746355	27.390923696229034	25.863539541852447	23.593335379172157
28-29	22.794781305846886	26.001408018484756	26.28842717114645	24.915383504521905
30-31	22.90895716374533	25.69746556672744	26.873296387891045	24.52028088163619
32-33	23.315341986028123	26.165451197718287	26.148076610646786	24.371130205606804
34-35	23.48119031716519	26.60455439825261	25.8030669530841	24.111188331498095
36-37	24.21836922577035	26.892024838890194	25.49235518168854	23.39725075365092
38-39	22.47188475910248	26.378459122335148	27.13933064967417	24.010325468888208
40-41	22.45338195209127	25.48648843800206	27.849432279726337	24.210697330180334
42-43	23.631469213134285	25.464600971171723	27.23748578442876	23.66644403126523
44-45	21.97727313753452	26.543630521508383	27.819647273318047	23.65944906763904
46-47	23.190109572720544	25.07807281982779	26.956333375453546	24.775484231998124
48-49	22.67135404444284	24.774356012058416	27.432442190010285	25.121847753488456
50-51	21.654602234778057	25.15366355578822	28.305910067332167	24.88582414210156
52-53	21.187067891763093	24.726068198638913	30.007040092423775	24.079823817174216
54-55	20.427324584363774	25.307552755564384	28.942903045291263	25.322219614780582
56-57	21.431891618679714	25.311839991335273	27.715399750889034	25.540868639095983
58-59	20.54759282993664	25.011507843385022	29.550111016842067	24.890788309836275
60-61	21.33328519594924	26.256611368846688	27.521571565247214	24.888531869956857
62-63	19.734371897395167	27.373549109157537	27.336769139123057	25.555309854324243
64-65	20.01958589815333	27.81468310558333	26.537086845858077	25.628644150405254
66-67	19.934969402675236	27.738866725634963	26.16274346986299	26.163420401826816
68-69	20.091566330306694	27.78286730328357	25.78749751791613	26.338068848493602
70-71	20.597234507283787	27.08404787262848	25.46572919111143	26.8529884289763
72-73	20.26621477697348	25.813220932541476	25.688214163221833	28.23235012726321
74-75	20.351508204415403	25.679188403704174	25.63315703016409	28.33614636171634
76	20.874776612451935	25.58757694459989	25.407964330198386	28.129682112749787
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	2.0
12	4.0
13	5.5
14	9.5
15	20.0
16	39.0
17	50.0
18	80.0
19	149.5
20	238.0
21	287.0
22	337.0
23	525.0
24	765.0
25	867.0
26	1063.5
27	1437.5
28	1819.5
29	2024.0
30	2223.0
31	2695.0
32	3462.5
33	3957.0
34	4422.0
35	5374.5
36	6586.0
37	7310.0
38	7661.0
39	8935.0
40	10551.0
41	11606.5
42	11969.0
43	12433.0
44	13165.5
45	13688.5
46	13943.0
47	13439.5
48	12408.0
49	11153.5
50	10427.0
51	9670.5
52	8021.5
53	6762.5
54	6396.0
55	6071.0
56	5155.0
57	4390.0
58	4216.0
59	3761.0
60	3105.0
61	2815.0
62	2726.0
63	2598.5
64	2387.0
65	2314.5
66	2285.5
67	2245.0
68	2120.0
69	1982.5
70	1905.5
71	1841.0
72	1763.0
73	1621.5
74	1457.5
75	1357.0
76	1213.5
77	962.0
78	824.5
79	795.0
80	693.0
81	503.5
82	364.5
83	313.0
84	236.5
85	141.5
86	89.5
87	56.0
88	43.0
89	21.0
90	6.5
91	1.5
92	2.0
93	2.5
94	2.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	221588.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.205299869356644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.46999999999998	26.883591832306386
2	21.23699999999999	19.20049906651053
3	8.797999999999996	11.931486847517988
4	3.6249999999999982	6.554768481056711
5	1.7069999999999992	3.858272343849588
6	1.0030000000000499	2.7204549461380183
7	0.6799999999999999	2.151772273781376
8	0.5319999999999997	1.9239375624398178
9	0.4209999999999998	1.7128288120499224
>10	2.483999999999999	19.814839091735088
>50	0.03599999999999998	1.0225438830448468
>100	0.0059999999999999975	0.6057510182493788
>500	0.0	0.0
>1k	9.999999999999996E-4	1.6192538413203543
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT	3582	1.616513529613517	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG	419	0.18908966189504847	No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGACCTCGTATGCCGT	285	0.12861707312670362	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC	248	0.11191941801902631	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.512879758831706E-4	0.0
3	0.0	0.0	0.0	4.512879758831706E-4	0.0
4	0.0	0.0	0.0	0.0018051519035326823	0.0
5	0.0	0.0	0.0	0.0018051519035326823	0.0
6	0.0	0.0	0.0	0.0018051519035326823	0.0
7	0.0	0.0	0.0	0.0018051519035326823	0.0
8	0.0	0.0	0.0	0.0018051519035326823	0.0
9	0.0	0.0	0.0	0.0027077278552990235	0.0
10	0.0	0.0	0.0	0.0027077278552990235	0.0
11	0.0	0.0	0.0	0.003159015831182194	0.0
12	0.0	0.0	0.0	0.003159015831182194	0.0
13	0.0	0.0	0.0	0.004061591782948535	0.0
14	0.0	0.0	0.0	0.004061591782948535	0.0
15	0.0	0.0	0.0	0.004061591782948535	0.0
16	0.0	0.0	0.0	0.004512879758831706	0.0
17	0.0	0.0	0.0	0.004512879758831706	0.0
18	0.0	0.0	0.0	0.004964167734714876	0.0
19	0.0	0.0	0.0	0.005415455710598047	0.0
20	0.0	0.0	0.0	0.005866743686481217	0.0
21	0.0	0.0	0.0	0.006318031662364388	0.0
22	0.0	0.0	0.0	0.007220607614130729	0.0
23	0.0	0.0	0.0	0.00857447154178024	0.0
24	0.0	0.0	0.0	0.009025759517663412	0.0
25	0.0	0.0	0.0	0.012184775348845605	0.0
26	0.0	0.0	0.0	0.014441215228261459	0.0
27	0.0	0.0	0.0	0.02482083867357438	0.0
28	0.0	0.0	0.0	0.03565175009477047	0.0
29	0.0	0.0	0.0	0.050544253298915105	0.0
30	0.0	0.0	0.0	0.0658880444789429	0.0
31	0.0	0.0	0.0	0.08258569958662021	0.0
32	0.0	0.0	0.0	0.1028936585013629	0.0
33	0.0	0.0	0.0	0.14937632001732945	0.0
34	0.0	0.0	0.0	0.21661822842392187	0.0
35	0.0	0.0	0.0	0.3064245356246728	0.0
36	0.0	0.0	0.0	0.4558008556420023	0.0
37	0.0	0.0	0.0	0.7080708341606946	0.0
38	0.0	0.0	0.0	0.9833564994494287	0.0
39	0.0	0.0	0.0	1.3168583136270917	0.0
40	0.0	0.0	0.0	1.698647941224254	0.0
41	0.0	0.0	0.0	2.0885607523873135	0.0
42	0.0	0.0	0.0	2.510966297813961	0.0
43	0.0	0.0	0.0	3.0326551979349063	0.0
44	0.0	0.0	0.0	3.6360272216907052	0.0
45	0.0	0.0	0.0	4.359441847031428	0.0
46	0.0	0.0	0.0	5.161831868151705	0.0
47	0.0	0.0	0.0	5.957452569633735	0.0
48	0.0	0.0	0.0	6.749914255284582	0.0
49	0.0	0.0	0.0	7.515749950358322	0.0
50	0.0	0.0	0.0	8.211184721194288	0.0
51	0.0	0.0	0.0	8.882249941332564	0.0
52	0.0	0.0	0.0	9.543386826001408	0.0
53	0.0	0.0	0.0	10.201815982814955	0.0
54	0.0	0.0	0.0	10.863404155459682	0.0
55	0.0	0.0	0.0	11.628337274581655	0.0
56	0.0	0.0	0.0	12.405003881076592	0.0
57	0.0	0.0	0.0	13.148726465332057	0.0
58	0.0	0.0	0.0	13.840099644385075	0.0
59	0.0	0.0	0.0	14.578406772929942	0.0
60	0.0	0.0	0.0	15.258046464609997	0.0
61	0.0	0.0	0.0	15.875408415618175	0.0
62	0.0	0.0	0.0	16.47517013556691	0.0
63	0.0	0.0	0.0	17.11599906132101	0.0
64	0.0	0.0	0.0	17.714858205317977	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCAC	15	0.002222528	70.00001	34
GAGACGA	20	7.9145204E-5	70.0	53
CGAGAAT	20	7.9145204E-5	70.0	14
ACTCGTA	35	2.9649345E-7	60.0	38
TCCGGAT	20	0.0069446694	52.5	3
GTCTATA	20	0.0069446694	52.5	1
AGCGCAC	20	0.0069446694	52.5	24
ACGGTAA	20	0.0069446694	52.5	13
TATGCCG	630	0.0	51.666664	43
CGTATGC	635	0.0	51.259842	41
TCGTATG	640	0.0	50.859375	40
CTCGTAT	620	0.0	50.241936	39
TCTCGTA	555	0.0	49.819817	38
GTATGCC	650	0.0	49.538464	42
TGCCGTC	650	0.0	49.538464	45
ATGCCGT	655	0.0	49.16031	44
CCGTCTT	635	0.0	49.05512	47
GCCGTCT	645	0.0	48.83721	46
ATCTCGT	560	0.0	48.75	37
TCGCAGA	685	0.0	48.029198	31
>>END_MODULE