FastQCFastQC Report
Wed 25 May 2016
SRR1781197_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781197_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108833
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGTT150.0022194870.055
CACTGAC200.006935217452.554
TGACATG200.006935217452.557
TGCAGAG400.002409851935.047
CCCGATC400.002409851935.033
GTATGGA450.00429150331.1111092
TGCAAGC450.00429150331.11110953
ATCCTCT500.007182162727.99999824
CTAGAGG500.007182162727.9999983
GGGTGTA500.007182162727.9999981
ACTATAG500.007182162727.9999983
GAGCTAA500.007182162727.99999841
GATATAG500.007182162727.9999981
TATAGTG500.007182162727.9999985
AGGGGGG500.007182162727.99999810
GACTGGG500.007182162727.99999832
ACTTTCC500.007182162727.99999864
CTATAGT657.912864E-426.9230774
CTGTTCT808.600083E-526.2513
CTCTAGA808.600083E-526.251
Produced by FastQC (version 0.11.5)