##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781197_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	108833
Sequences flagged as poor quality	0
Sequence length	76
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.613913059458067	34.0	32.0	34.0	21.0	34.0
2	30.76240662299119	34.0	32.0	34.0	21.0	34.0
3	30.72904358053164	34.0	32.0	34.0	21.0	34.0
4	30.86325838670256	34.0	32.0	34.0	21.0	34.0
5	30.772403590822638	34.0	32.0	34.0	21.0	34.0
6	33.440280062113516	37.0	34.0	38.0	21.0	38.0
7	33.44526016925013	37.0	34.0	38.0	21.0	38.0
8	33.185973004511496	37.0	34.0	38.0	21.0	38.0
9	33.56781490908089	37.0	34.0	38.0	21.0	38.0
10-11	33.36729668391021	37.0	34.0	38.0	21.0	38.0
12-13	33.322278169305264	37.0	34.0	38.0	21.0	38.0
14-15	33.17665138331205	37.0	34.0	38.0	21.0	38.0
16-17	33.114588406090064	37.0	33.5	38.0	21.0	38.0
18-19	33.14547058337085	37.0	34.0	38.0	21.0	38.0
20-21	33.064355480414946	37.0	33.5	38.0	21.0	38.0
22-23	33.11735411134491	37.0	33.5	38.0	21.0	38.0
24-25	33.109098343333365	37.0	33.5	38.0	21.0	38.0
26-27	33.05400475958579	37.0	33.0	38.0	21.0	38.0
28-29	33.1179927044187	37.0	34.0	38.0	21.0	38.0
30-31	33.14001727417235	37.0	34.0	38.0	21.0	38.0
32-33	33.12041843926015	37.0	33.5	38.0	21.0	38.0
34-35	33.149858039381435	37.0	34.0	38.0	21.0	38.0
36-37	33.00159877978187	37.0	33.5	38.0	21.0	38.0
38-39	32.96247002287909	37.0	33.0	38.0	21.0	38.0
40-41	33.00390506555916	37.0	33.5	38.0	21.0	38.0
42-43	33.06636314353183	37.0	33.5	38.0	21.0	38.0
44-45	33.1270800216846	37.0	34.0	38.0	21.0	38.0
46-47	33.10213354405373	37.0	34.0	38.0	21.0	38.0
48-49	33.035719864379374	37.0	33.5	38.0	21.0	38.0
50-51	32.90356785166264	37.0	32.5	38.0	21.0	38.0
52-53	32.97430926281551	37.0	33.0	38.0	21.0	38.0
54-55	32.96893864912297	37.0	33.0	38.0	21.0	38.0
56-57	32.91773175415545	37.0	33.0	38.0	20.5	38.0
58-59	32.92601508733564	37.0	32.5	38.0	21.0	38.0
60-61	32.89448972278629	37.0	32.5	38.0	20.5	38.0
62-63	32.849843337958156	37.0	32.0	38.0	19.5	38.0
64-65	32.84800566004796	37.0	32.0	38.0	19.5	38.0
66-67	32.73804360809681	37.0	32.0	38.0	14.5	38.0
68-69	32.50646862624388	37.0	31.0	38.0	13.5	38.0
70-71	32.605280567474935	37.0	31.0	38.0	11.0	38.0
72-73	32.583494895849604	37.0	31.0	38.0	11.0	38.0
74-75	32.53650087749121	37.0	31.0	38.0	11.0	38.0
76	31.1182729503	36.0	27.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	7.0
13	30.0
14	137.0
15	376.0
16	840.0
17	1465.0
18	1861.0
19	1937.0
20	1642.0
21	1375.0
22	1282.0
23	1288.0
24	1480.0
25	1626.0
26	1882.0
27	2254.0
28	2677.0
29	3171.0
30	3666.0
31	4286.0
32	5171.0
33	6269.0
34	7627.0
35	9964.0
36	15076.0
37	31443.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.89263366809699	18.284895206417172	9.679049552984848	27.143421572500987
2	23.184144514990855	18.547683147574727	31.59427747098766	26.67389486644676
3	20.71522424264699	20.30082787389854	27.886762287173926	31.09718559628054
4	15.563294221421812	15.188407927742503	33.83165032664725	35.41664752418844
5	15.452114707855156	32.060128821221504	33.720470813080595	18.76728565784275
6	32.26043571343251	33.824299615006474	16.69806033096579	17.217204340595224
7	30.774673123041723	26.32473606351015	21.184750948701218	21.715839864746904
8	26.551689285418945	32.1115838027069	19.400365697904128	21.936361213970027
9	25.079709279354606	14.034346200141501	20.456111657309823	40.42983286319407
10-11	27.41907325902989	22.277250466310768	25.926878795953435	24.376797478705907
12-13	26.20115222404969	20.85121240800125	25.03928036533037	27.90835500261869
14-15	24.886753098784375	21.85228744957871	22.414616890097673	30.84634256153924
16-17	25.81064566813375	24.044177776961032	23.766688412521937	26.378488142383283
18-19	25.080168698832157	23.835141914676612	24.332693208861283	26.751996177629945
20-21	25.175268530684626	23.989966278610346	24.2403498938741	26.594415296830924
22-23	25.372818906030343	23.70604504148558	24.654746262622552	26.266389789861535
24-25	24.960260215192083	23.9141620648149	24.26791506252699	26.857662657466026
26-27	24.276184613122858	23.97939963062674	24.836216956254077	26.908198799996324
28-29	25.02871371734676	23.60818869276782	25.331471153051005	26.031626436834415
30-31	24.964854409967565	23.748771052897556	24.555511655472145	26.73086288166273
32-33	24.75489970872805	23.87832734556614	24.702985307765108	26.6637876379407
34-35	25.65168652890208	23.319213841390017	25.025957200481468	26.00314242922643
36-37	25.411869561621934	23.018753503073516	24.487517572795017	27.08185936250953
38-39	24.380472834526294	23.763013056701553	25.626877877114477	26.229636231657675
40-41	24.849999540580523	22.58092674097011	26.295333216947064	26.273740501502303
42-43	24.411713358999567	22.71369896998153	25.778945724182922	27.09564194683598
44-45	23.416610770630232	23.124879402387144	26.37205626969761	27.08645355728502
46-47	23.60818869276782	23.342644234744977	26.56639070870049	26.48277636378672
48-49	23.536519254270306	23.295783448035063	26.66608473532844	26.501612562366194
50-51	23.41477309272004	23.525952606286697	26.169452280098866	26.8898220208944
52-53	22.846930618470502	23.504359890841933	27.018459474607887	26.630250016079682
54-55	22.710483033638695	23.681236389697975	26.68308325599772	26.925197320665607
56-57	22.76653220989957	23.902676577876196	26.307737542840865	27.02305366938337
58-59	23.000376723971588	23.500684535021545	26.307737542840865	27.191201198166
60-61	22.524877564709232	23.17587496439499	26.561337094447456	27.737910376448323
62-63	22.33513732048184	23.061020095007947	26.732700559572926	27.87114202493729
64-65	23.11569101283618	23.48184833644207	25.923662859610598	27.478797791111155
66-67	22.991647753898174	23.50252221293174	25.659037240542848	27.846792792627234
68-69	22.693025093491865	23.887515735117105	25.32044508558985	28.099014085801183
70-71	22.837282809441987	23.855815791166282	25.554749019139418	27.752152380252316
72-73	22.692106254536768	23.4496889730137	25.761487784036092	28.09671698841344
74-75	22.529012340007167	23.613701726498395	25.93560776602685	27.92167816746759
76	23.043562154861117	23.51768305569083	25.72657190374243	27.712182885705623
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	0.5
11	0.0
12	0.0
13	1.0
14	4.5
15	5.0
16	7.0
17	11.0
18	21.5
19	32.0
20	37.5
21	43.0
22	65.5
23	109.0
24	199.5
25	269.0
26	310.0
27	383.5
28	488.0
29	560.0
30	681.5
31	903.0
32	1257.5
33	1512.0
34	1634.5
35	1998.5
36	2574.5
37	2909.0
38	3239.5
39	3975.0
40	4681.0
41	5163.5
42	5345.0
43	5563.5
44	5786.0
45	5802.0
46	5814.0
47	5644.5
48	5215.5
49	4645.0
50	4334.0
51	4132.0
52	3556.0
53	3094.5
54	3007.0
55	2810.0
56	2426.0
57	2088.0
58	1937.0
59	1873.5
60	1775.0
61	1736.0
62	1732.0
63	1736.0
64	1805.0
65	1866.0
66	1901.5
67	1941.0
68	1942.0
69	1956.5
70	1910.0
71	1850.0
72	1825.0
73	1782.5
74	1737.0
75	1709.0
76	1558.5
77	1272.0
78	1053.5
79	971.0
80	844.0
81	619.0
82	431.0
83	341.0
84	277.5
85	174.5
86	103.5
87	72.0
88	53.0
89	28.0
90	16.5
91	7.5
92	4.0
93	4.0
94	2.5
95	1.0
96	1.0
97	0.5
98	0.0
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	108833.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.13402184999035
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.41035894355845	42.67639410840462
2	13.094880589230112	15.225161485946359
3	5.707376440278809	9.953782400558655
4	2.705906526102831	6.292209164499737
5	1.4572697529595853	4.235847582994129
6	0.821887496246187	2.866777539900582
7	0.5911267761462959	2.405520384442219
8	0.4046215366135074	1.8817821800373047
9	0.3508827387820259	1.8358402322824878
>10	1.4556892000821888	12.62668492093391
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.188389550963403E-4	0.0
2	0.0	0.0	0.0	9.188389550963403E-4	0.0
3	0.0	0.0	0.0	9.188389550963403E-4	0.0
4	0.0	0.0	0.0	0.0027565168652890206	0.0
5	0.0	0.0	0.0	0.003675355820385361	0.0
6	0.0	0.0	0.0	0.003675355820385361	0.0
7	0.0	0.0	0.0	0.003675355820385361	0.0
8	0.0	0.0	0.0	0.003675355820385361	0.0
9	0.0	0.0	0.0	0.003675355820385361	0.0
10	0.0	0.0	0.0	0.003675355820385361	0.0
11	0.0	0.0	0.0	0.003675355820385361	0.0
12	0.0	0.0	0.0	0.003675355820385361	0.0
13	0.0	0.0	0.0	0.003675355820385361	0.0
14	0.0	0.0	0.0	0.003675355820385361	0.0
15	0.0	0.0	0.0	0.003675355820385361	0.0
16	0.0	0.0	0.0	0.003675355820385361	0.0
17	0.0	0.0	0.0	0.003675355820385361	0.0
18	0.0	0.0	0.0	0.003675355820385361	0.0
19	0.0	0.0	0.0	0.003675355820385361	0.0
20	0.0	0.0	0.0	0.005513033730578041	0.0
21	0.0	0.0	0.0	0.006431872685674382	0.0
22	0.0	0.0	0.0	0.011026067461156083	0.0
23	0.0	0.0	0.0	0.014701423281541445	0.0
24	0.0	0.0	0.0	0.018376779101926807	0.0
25	0.0	0.0	0.0	0.018376779101926807	0.0
26	0.0	0.0	0.0	0.020214457012119484	0.0
27	0.0	0.0	0.0	0.04410426984462433	0.0
28	0.0	0.0	0.0	0.07166943849751455	0.0
29	0.0	0.0	0.0	0.10107228506059743	0.0
30	0.0	0.0	0.0	0.12404325893800594	0.0
31	0.0	0.0	0.0	0.1415011990848364	0.0
32	0.0	0.0	0.0	0.17549824042340098	0.0
33	0.0	0.0	0.0	0.2581937463820716	0.0
34	0.0	0.0	0.0	0.36202254830795805	0.0
35	0.0	0.0	0.0	0.5209816875396249	0.0
36	0.0	0.0	0.0	0.7580421379544807	0.0
37	0.0	0.0	0.0	1.0814734501483925	0.0
38	0.0	0.0	0.0	1.4352264478604835	0.0
39	0.0	0.0	0.0	1.8128692584050794	0.0
40	0.0	0.0	0.0	2.2217525934229507	0.0
41	0.0	0.0	0.0	2.6462561906774598	0.0
42	0.0	0.0	0.0	3.058814881515717	0.0
43	0.0	0.0	0.0	3.543043010851488	0.0
44	0.0	0.0	0.0	4.1329376200233385	0.0
45	0.0	0.0	0.0	4.691591704721914	0.0
46	0.0	0.0	0.0	5.298944254040594	0.0
47	0.0	0.0	0.0	5.946725717383514	0.0
48	0.0	0.0	0.0	6.545808716106328	0.0
49	0.0	0.0	0.0	7.100787444984517	0.0
50	0.0	0.0	0.0	7.5592880835775915	0.0
51	0.0	0.0	0.0	8.083026287982506	0.0
52	0.0	0.0	0.0	8.611358687162902	0.0
53	0.0	0.0	0.0	9.100181011274154	0.0
54	0.0	0.0	0.0	9.600029402846562	0.0
55	0.0	0.0	0.0	10.197274723659184	0.0
56	0.0	0.0	0.0	10.78808817178613	0.0
57	0.0	0.0	0.0	11.385333492598752	0.0
58	0.0	0.0	0.0	11.938474543566748	0.0
59	0.0	0.0	0.0	12.474157654387916	0.0
60	0.0	0.0	0.0	13.019029154760045	0.0
61	0.0	0.0	0.0	13.449964624700229	0.0
62	0.0	0.0	0.0	13.921329008664651	0.0
63	0.0	0.0	0.0	14.450580246800143	0.0
64	0.0	0.0	0.0	15.000045941947755	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGTGTT	15	0.00221948	70.0	55
CACTGAC	20	0.0069352174	52.5	54
TGACATG	20	0.0069352174	52.5	57
TGCAGAG	40	0.0024098519	35.0	47
CCCGATC	40	0.0024098519	35.0	33
GTATGGA	45	0.004291503	31.111109	2
TGCAAGC	45	0.004291503	31.111109	53
ATCCTCT	50	0.0071821627	27.999998	24
CTAGAGG	50	0.0071821627	27.999998	3
GGGTGTA	50	0.0071821627	27.999998	1
ACTATAG	50	0.0071821627	27.999998	3
GAGCTAA	50	0.0071821627	27.999998	41
GATATAG	50	0.0071821627	27.999998	1
TATAGTG	50	0.0071821627	27.999998	5
AGGGGGG	50	0.0071821627	27.999998	10
GACTGGG	50	0.0071821627	27.999998	32
ACTTTCC	50	0.0071821627	27.999998	64
CTATAGT	65	7.912864E-4	26.923077	4
CTGTTCT	80	8.600083E-5	26.25	13
CTCTAGA	80	8.600083E-5	26.25	1
>>END_MODULE