FastQCFastQC Report
Wed 25 May 2016
SRR1781197_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781197_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98619
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT52395.3123637432949025No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG5400.5475618288565084No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC3450.34983116843610257No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATACCTCGTATGCCGT2870.29101897200336646No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATAACTCGTATGCCGT1660.16832456220403777No Hit
ATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTCTT1290.13080643689349922Illumina PCR Primer Index 6 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAG150.002218860270.000011
TTAGTCG150.002218860270.0000115
ATAACTC207.892781E-570.035
CTCGTAT8050.056.95652439
CTGCTTG7850.056.62420354
CGTATGC8150.056.2576741
TCGTATG8150.056.2576740
CTTCTGC7850.056.1783451
TATGCCG8100.056.1728443
CTATCGG252.3802955E-455.99999638
AACTCGT252.3802955E-455.99999637
TAACTCG252.3802955E-455.99999636
GTATGCC8200.055.91463542
TCTCGTA7600.055.72368238
TCTGCTT7800.055.64102653
TATCTCG7650.055.35947836
GCCGTCT8000.055.12499646
CGTCTTC7750.055.09677548
CCGTCTT7750.055.09677547
TCTTCTG7900.054.93670750