##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781197_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	98619
Sequences flagged as poor quality	0
Sequence length	76
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.28561433395188	34.0	34.0	34.0	32.0	34.0
2	33.29609912897109	34.0	34.0	34.0	33.0	34.0
3	33.35093643212768	34.0	34.0	34.0	33.0	34.0
4	33.41142173414859	34.0	34.0	34.0	34.0	34.0
5	33.39899005262678	34.0	34.0	34.0	34.0	34.0
6	36.82808586580679	38.0	37.0	38.0	34.0	38.0
7	36.71942526288038	38.0	37.0	38.0	34.0	38.0
8	36.50654539186161	38.0	37.0	38.0	34.0	38.0
9	36.478660298725394	38.0	37.0	38.0	34.0	38.0
10-11	36.38492075563532	38.0	37.0	38.0	34.0	38.0
12-13	36.29937435991036	38.0	37.0	38.0	34.0	38.0
14-15	36.30888064166135	38.0	37.0	38.0	34.0	38.0
16-17	36.21725529563268	38.0	37.0	38.0	33.5	38.0
18-19	36.20334316916619	38.0	37.0	38.0	33.5	38.0
20-21	36.15058964296941	38.0	36.5	38.0	33.0	38.0
22-23	36.23134487269188	38.0	37.0	38.0	34.0	38.0
24-25	36.205558766566284	38.0	37.0	38.0	34.0	38.0
26-27	35.982087630172686	38.0	36.5	38.0	31.5	38.0
28-29	35.94958882162666	38.0	36.0	38.0	31.5	38.0
30-31	36.014616858820304	38.0	37.0	38.0	32.0	38.0
32-33	35.88785629544003	38.0	36.0	38.0	31.0	38.0
34-35	35.87189081211531	38.0	36.0	38.0	31.0	38.0
36-37	35.817190399415935	38.0	36.0	38.0	31.0	38.0
38-39	35.455612001744086	38.0	36.0	38.0	31.0	38.0
40-41	35.75094048814123	38.0	36.0	38.0	31.0	38.0
42-43	35.88251756760867	38.0	36.0	38.0	31.5	38.0
44-45	35.859915432117546	38.0	36.0	38.0	31.0	38.0
46-47	35.85526115657227	38.0	36.0	38.0	31.5	38.0
48-49	35.813144525902715	38.0	36.0	38.0	31.0	38.0
50-51	35.676025917926566	38.0	36.0	38.0	31.0	38.0
52-53	35.68577049047344	38.0	36.0	38.0	31.0	38.0
54-55	35.690708686967014	38.0	36.0	38.0	31.0	38.0
56-57	35.641595432928746	38.0	36.0	38.0	31.0	38.0
58-59	35.57380423650615	38.0	36.0	38.0	31.0	38.0
60-61	35.549752076171934	38.0	36.0	38.0	31.0	38.0
62-63	35.56865309930136	38.0	36.0	38.0	31.0	38.0
64-65	35.53499832689441	38.0	36.0	38.0	31.0	38.0
66-67	35.399106663016255	38.0	36.0	38.0	30.5	38.0
68-69	35.142219045011615	38.0	36.0	38.0	27.5	38.0
70-71	34.67521978523408	38.0	35.5	38.0	25.5	38.0
72-73	34.300743264482506	38.0	35.0	38.0	24.0	38.0
74-75	34.295404536651155	38.0	35.0	38.0	24.0	38.0
76	33.39189202891938	37.0	34.0	38.0	22.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	4.0
22	20.0
23	54.0
24	96.0
25	230.0
26	388.0
27	705.0
28	1063.0
29	1662.0
30	2410.0
31	3521.0
32	4803.0
33	6469.0
34	8490.0
35	11430.0
36	15976.0
37	41298.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.17544286597917	16.463358987619017	9.134142508035977	32.22705563836583
2	22.196534136424013	17.394214096675086	34.93647268781878	25.472779079082127
3	19.874466380717713	19.365436680558513	31.808272239629282	28.951824699094498
4	14.724343179306217	20.256745657530494	32.38828217686247	32.63062898630081
5	15.040712235978868	31.22522029223578	37.54043338504751	16.19363408673785
6	31.19074417708555	38.82821768624707	15.15732262545757	14.823715511209807
7	29.051197030998082	25.537675295835488	19.156551982883624	26.254575690282806
8	24.992141473752522	37.21088228434683	16.653991624332026	21.142984617568622
9	23.79967349090946	13.632261531753517	17.768381346393696	44.79968363094333
10-11	26.195763493850073	24.819253896308012	26.09841916872002	22.886563441121893
12-13	25.16705705797057	19.563167340978918	26.131881280483476	29.13789432056703
14-15	23.548200650990175	20.210101501739018	20.71152617649743	35.53017167077338
16-17	27.75479370101096	26.135937294030565	20.86007767265943	25.24919133229905
18-19	27.762398726411746	22.801387156633105	21.767610703819752	27.6686034131354
20-21	24.000953163183564	22.597065474198683	21.570894046786115	31.831087315831635
22-23	24.372078402741863	25.557955363570915	21.846195966294527	28.223770267392695
24-25	27.768482746732374	25.632484612498608	21.510560845273226	25.0884717954958
26-27	27.204696863687527	26.244942658108478	22.07941674525193	24.47094373295207
28-29	24.300591163974488	25.780529106967215	22.51138218801651	27.407497541041785
30-31	24.206288849004757	25.853030349121365	22.02922357760675	27.911457224267128
32-33	26.95677303562194	22.90532250377716	21.730599580202597	28.4073048803983
34-35	24.363966375647696	24.95513035013537	22.50073515245541	28.18016812176153
36-37	24.030359261399934	25.371378740404992	25.554406351717212	25.04385564647786
38-39	23.74238229955688	22.939291617234	26.095377158559707	27.22294892464941
40-41	23.661262028615177	21.29001510865046	26.955252030541782	28.09347083219258
42-43	27.126618602906134	21.50042081140551	26.29412182236689	25.07883876332147
44-45	23.027509911883108	25.341972642188626	27.22142791956925	24.40908952635902
46-47	26.44419432360904	22.01401352680518	24.009065190277738	27.532726959308047
48-49	25.829708271225627	21.59370912298847	23.785984445188046	28.79059816059786
50-51	22.73547693649297	21.831492917186342	27.028767276082704	28.404262870237986
52-53	21.948103306665047	21.784848761394862	31.154240055161786	25.11280787677831
54-55	21.594723126375243	21.91819020675529	27.980409454567578	28.506677212301888
56-57	25.260345369553534	21.992719455682984	24.20730285239153	28.539632322371954
58-59	21.952666321905514	21.926302233849462	30.233017978280046	25.888013465964978
60-61	24.761962704955433	25.346028655735708	24.01210720043805	25.879901438870807
62-63	20.90976383861122	29.50901956012533	23.51220353075979	26.069013070503654
64-65	20.903679818290595	30.234538983360203	23.001145823827052	25.86063537452215
66-67	21.49940680801874	29.906508887739687	22.317707541143186	26.27637676309839
68-69	20.93663492836066	30.58234214502276	22.063192691063588	26.417830235552987
70-71	21.19824780214766	28.85904338920492	22.900759488536693	27.04194932011073
72-73	21.730092578509215	25.871789411776636	23.47874141899634	28.919376590717818
74-75	21.618045204270985	25.75010900536408	23.86507670935621	28.766769081008732
76	21.68344842271773	25.52347924842069	23.446800312313044	29.346272016548536
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	2.5
16	8.5
17	14.0
18	18.0
19	21.5
20	31.0
21	41.0
22	47.0
23	91.0
24	158.5
25	188.0
26	223.0
27	302.0
28	407.5
29	469.0
30	524.0
31	658.5
32	965.5
33	1193.0
34	1297.0
35	1601.5
36	2068.0
37	2334.0
38	2527.0
39	3064.5
40	4069.5
41	5403.5
42	6077.0
43	6496.0
44	6610.0
45	6010.0
46	5715.0
47	5439.5
48	4636.5
49	3985.0
50	3861.0
51	3546.5
52	2849.0
53	2396.0
54	2326.0
55	2193.0
56	1914.5
57	1660.5
58	1552.0
59	1476.5
60	1429.5
61	1441.0
62	1424.0
63	1500.5
64	1669.5
65	1748.0
66	1785.0
67	1836.0
68	1830.5
69	1780.0
70	1755.5
71	1776.0
72	1778.0
73	1662.5
74	1559.0
75	1573.0
76	1457.5
77	1234.0
78	976.5
79	827.0
80	752.5
81	566.5
82	389.5
83	324.0
84	261.5
85	158.0
86	95.0
87	73.0
88	51.0
89	18.5
90	8.0
91	5.5
92	3.0
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	98619.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.291667934170896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.96978021978022	26.118699236455452
2	20.215201465201467	17.907299810381367
3	8.869047619047619	11.784747361056185
4	4.242216117216118	7.5157931027489635
5	2.1314102564102564	4.720185765420457
6	1.353021978021978	3.595656009491072
7	0.9134615384615385	2.8321114592522743
8	0.7005494505494506	2.4822802908161714
9	0.4601648351648352	1.8343321266693031
>10	2.1222527472527473	14.14636124884657
>50	0.009157509157509158	0.26262687717376976
>100	0.009157509157509158	0.939981139537006
>500	0.0022893772893772895	0.5475618288565084
>1k	0.0	0.0
>5k	0.0022893772893772895	5.3123637432949025
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT	5239	5.3123637432949025	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG	540	0.5475618288565084	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC	345	0.34983116843610257	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATACCTCGTATGCCGT	287	0.29101897200336646	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATAACTCGTATGCCGT	166	0.16832456220403777	No Hit
ATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTCTT	129	0.13080643689349922	Illumina PCR Primer Index 6 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.001014003386771312	0.0
2	0.0	0.0	0.0	0.001014003386771312	0.0
3	0.0	0.0	0.0	0.001014003386771312	0.0
4	0.0	0.0	0.0	0.0030420101603139352	0.0
5	0.0	0.0	0.0	0.004056013547085248	0.0
6	0.0	0.0	0.0	0.004056013547085248	0.0
7	0.0	0.0	0.0	0.004056013547085248	0.0
8	0.0	0.0	0.0	0.004056013547085248	0.0
9	0.0	0.0	0.0	0.004056013547085248	0.0
10	0.0	0.0	0.0	0.004056013547085248	0.0
11	0.0	0.0	0.0	0.004056013547085248	0.0
12	0.0	0.0	0.0	0.004056013547085248	0.0
13	0.0	0.0	0.0	0.004056013547085248	0.0
14	0.0	0.0	0.0	0.005070016933856559	0.0
15	0.0	0.0	0.0	0.005070016933856559	0.0
16	0.0	0.0	0.0	0.0060840203206278705	0.0
17	0.0	0.0	0.0	0.0060840203206278705	0.0
18	0.0	0.0	0.0	0.0060840203206278705	0.0
19	0.0	0.0	0.0	0.0060840203206278705	0.0
20	0.0	0.0	0.0	0.0060840203206278705	0.0
21	0.0	0.0	0.0	0.007098023707399183	0.0
22	0.0	0.0	0.0	0.010140033867713118	0.0
23	0.0	0.0	0.0	0.01115403725448443	0.0
24	0.0	0.0	0.0	0.01115403725448443	0.0
25	0.0	0.0	0.0	0.01115403725448443	0.0
26	0.0	0.0	0.0	0.014196047414798366	0.0
27	0.0	0.0	0.0	0.03954613208408116	0.0
28	0.0	0.0	0.0	0.06895223030044921	0.0
29	0.0	0.0	0.0	0.10140033867713118	0.0
30	0.0	0.0	0.0	0.12472241657287135	0.0
31	0.0	0.0	0.0	0.14094647076121233	0.0
32	0.0	0.0	0.0	0.1804926028452935	0.0
33	0.0	0.0	0.0	0.2747949178150255	0.0
34	0.0	0.0	0.0	0.39748932761435424	0.0
35	0.0	0.0	0.0	0.5647998864316207	0.0
36	0.0	0.0	0.0	0.8203287398979913	0.0
37	0.0	0.0	0.0	1.1955099930033766	0.0
38	0.0	0.0	0.0	1.5848872935235603	0.0
39	0.0	0.0	0.0	1.9884606414585424	0.0
40	0.0	0.0	0.0	2.4143420639024935	0.0
41	0.0	0.0	0.0	2.8574615439215565	0.0
42	0.0	0.0	0.0	3.2934830002332207	0.0
43	0.0	0.0	0.0	3.8156947444204463	0.0
44	0.0	0.0	0.0	4.438292823898032	0.0
45	0.0	0.0	0.0	5.050750869507904	0.0
46	0.0	0.0	0.0	5.67334894898549	0.0
47	0.0	0.0	0.0	6.356787231669354	0.0
48	0.0	0.0	0.0	6.974315294213083	0.0
49	0.0	0.0	0.0	7.5654792687007575	0.0
50	0.0	0.0	0.0	8.066396941765786	0.0
51	0.0	0.0	0.0	8.624098804490007	0.0
52	0.0	0.0	0.0	9.180786663827456	0.0
53	0.0	0.0	0.0	9.68576035043957	0.0
54	0.0	0.0	0.0	10.21912613188128	0.0
55	0.0	0.0	0.0	10.840710207972094	0.0
56	0.0	0.0	0.0	11.47040631115708	0.0
57	0.0	0.0	0.0	12.132550522718747	0.0
58	0.0	0.0	0.0	12.714588466725479	0.0
59	0.0	0.0	0.0	13.284458370090956	0.0
60	0.0	0.0	0.0	13.841146229428407	0.0
61	0.0	0.0	0.0	14.297447753475497	0.0
62	0.0	0.0	0.0	14.788225392672812	0.0
63	0.0	0.0	0.0	15.338829231689633	0.0
64	0.0	0.0	0.0	15.90768513166834	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACAAG	15	0.0022188602	70.00001	1
TTAGTCG	15	0.0022188602	70.00001	15
ATAACTC	20	7.892781E-5	70.0	35
CTCGTAT	805	0.0	56.956524	39
CTGCTTG	785	0.0	56.624203	54
CGTATGC	815	0.0	56.25767	41
TCGTATG	815	0.0	56.25767	40
CTTCTGC	785	0.0	56.17834	51
TATGCCG	810	0.0	56.17284	43
CTATCGG	25	2.3802955E-4	55.999996	38
AACTCGT	25	2.3802955E-4	55.999996	37
TAACTCG	25	2.3802955E-4	55.999996	36
GTATGCC	820	0.0	55.914635	42
TCTCGTA	760	0.0	55.723682	38
TCTGCTT	780	0.0	55.641026	53
TATCTCG	765	0.0	55.359478	36
GCCGTCT	800	0.0	55.124996	46
CGTCTTC	775	0.0	55.096775	48
CCGTCTT	775	0.0	55.096775	47
TCTTCTG	790	0.0	54.936707	50
>>END_MODULE