FastQCFastQC Report
Wed 25 May 2016
SRR1781196_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781196_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258028
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCTTG200.006945959352.49999615
GCGTTTC305.870881E-446.6666687
CGTTTCC350.001253959540.0000048
GTACAAG556.679038E-638.1818161
GGTCGGT601.2128994E-535.00000411
CTGTACG400.002415969534.99999641
GACTTTA400.002415969534.9999967
CGGTAAG400.002415969534.99999628
GTCGGGA400.002415969534.9999969
TGTATAG751.4859033E-632.6666683
GCGTATC751.4859033E-632.66666847
CGTATCA652.0977399E-532.30769348
AAAGCGA552.9870702E-431.8181825
GCATAAG450.004302331231.11110960
GTTTATC450.004302331231.11110939
AAGCGGA450.004302331231.11110945
ACATAAG450.004302331231.11110926
TACATGC604.9737113E-429.1666682
TGTCTAA604.9737113E-429.16666813
CCTATGC604.9737113E-429.16666844
Produced by FastQC (version 0.11.5)