##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781196_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	258028
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.347578557365868	34.0	32.0	34.0	23.0	34.0
2	31.301688188878725	34.0	32.0	34.0	23.0	34.0
3	31.19500984389291	34.0	32.0	34.0	23.0	34.0
4	31.36314663524889	34.0	33.0	34.0	23.0	34.0
5	31.40752553986389	34.0	33.0	34.0	23.0	34.0
6	34.133059202877206	37.0	34.0	38.0	24.0	38.0
7	34.099427193947946	37.0	34.0	38.0	24.0	38.0
8	33.851093679755685	37.0	34.0	38.0	23.0	38.0
9	34.22386330165718	37.0	34.0	38.0	24.0	38.0
10-11	34.04835521726324	37.0	34.0	38.0	23.5	38.0
12-13	34.013002464848775	37.0	34.0	38.0	23.0	38.0
14-15	33.89237214565861	37.0	34.0	38.0	23.0	38.0
16-17	33.8573488148573	37.0	34.0	38.0	22.5	38.0
18-19	33.92469421923202	37.0	34.0	38.0	23.0	38.0
20-21	33.843803773233915	37.0	34.0	38.0	23.0	38.0
22-23	33.93425713488459	37.0	34.0	38.0	23.0	38.0
24-25	33.914536406901576	37.0	34.0	38.0	23.0	38.0
26-27	33.837810237648625	37.0	34.0	38.0	23.0	38.0
28-29	33.89854395646984	37.0	34.0	38.0	23.0	38.0
30-31	33.99010378718589	37.0	34.0	38.0	23.0	38.0
32-33	33.92195808206861	37.0	34.0	38.0	23.0	38.0
34-35	33.95310974002821	37.0	34.0	38.0	23.0	38.0
36-37	33.79744252561737	37.0	34.0	38.0	23.0	38.0
38-39	33.81738997318121	37.0	34.0	38.0	23.0	38.0
40-41	33.800122467329125	37.0	34.0	38.0	23.0	38.0
42-43	33.88525857658859	37.0	34.0	38.0	23.0	38.0
44-45	33.9062291689274	37.0	34.0	38.0	23.0	38.0
46-47	33.887719549816296	37.0	34.0	38.0	23.0	38.0
48-49	33.86106352798922	37.0	34.0	38.0	23.0	38.0
50-51	33.724973646270946	37.0	34.0	38.0	23.0	38.0
52-53	33.81816314508503	37.0	34.0	38.0	23.0	38.0
54-55	33.77497984714837	37.0	34.0	38.0	23.0	38.0
56-57	33.75617181081123	37.0	34.0	38.0	23.0	38.0
58-59	33.738125319732745	37.0	34.0	38.0	23.0	38.0
60-61	33.72092951152588	37.0	34.0	38.0	23.0	38.0
62-63	33.70057900692948	37.0	34.0	38.0	22.5	38.0
64-65	33.70050730928426	37.0	34.0	38.0	23.0	38.0
66-67	33.580045576449066	37.0	34.0	38.0	22.0	38.0
68-69	33.36989784054444	37.0	34.0	38.0	21.5	38.0
70-71	33.51280287410668	37.0	34.0	38.0	22.0	38.0
72-73	33.45779721580604	37.0	34.0	38.0	22.0	38.0
74-75	33.43426876152976	37.0	33.0	38.0	22.0	38.0
76	31.976002604368517	36.0	31.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	42.0
14	131.0
15	364.0
16	869.0
17	1655.0
18	2196.0
19	2385.0
20	2271.0
21	2250.0
22	2248.0
23	2683.0
24	3064.0
25	3735.0
26	4611.0
27	5305.0
28	6479.0
29	7498.0
30	8961.0
31	10600.0
32	12932.0
33	15728.0
34	19638.0
35	25489.0
36	37787.0
37	79104.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.862852093571234	18.823538530702095	9.752817523679601	25.560791852047064
2	23.684251321562	19.95326088641543	31.660905018060053	24.701582773962517
3	19.941634241245136	22.185576759111413	28.61084843505356	29.26194056458989
4	14.324026849799246	16.786937851706014	36.8219728091525	32.06706248934224
5	14.156990714186058	33.76881578743392	36.03717426015781	16.037019238222207
6	29.972716139333716	36.57742570573736	18.36002294324647	15.089835211682454
7	29.018556125691784	28.567054738245464	22.6514176756011	19.762971460461657
8	25.56970561334429	33.46962345171842	20.013719441300946	20.946951493636348
9	24.62562202551661	15.779682825119753	21.696094997442138	37.8986001519215
10-11	26.05434293952594	23.96019036693692	27.384043592168293	22.601423101368844
12-13	25.6059419907917	22.43826251414575	26.09774908149503	25.85804641356752
14-15	24.057854186367372	23.721456586106935	23.49512456012526	28.725564667400437
16-17	24.251050273613718	26.032252313702386	25.089137612972234	24.62755979971166
18-19	23.379826995519867	26.063838033081684	26.18708047188677	24.36925449951168
20-21	23.5406622537089	26.010936797556855	26.211883981583394	24.236516967150852
22-23	23.977436557272856	25.477273784240467	26.44112266885764	24.104166989629032
24-25	23.706148169966053	25.820841149022588	26.20297022028617	24.27004046072519
26-27	23.021726324274887	26.11712682344552	26.570953539925902	24.290193312353697
28-29	23.674756228006263	25.71135690700234	27.153836017796518	23.46005084719488
30-31	23.844117692653512	25.456733377772956	26.754654533616506	23.94449439595703
32-33	23.310842234176135	26.276024307439506	26.26904832033733	24.144085138047032
34-35	24.31499682205032	25.402088145472586	26.46999550436387	23.812919528113227
36-37	24.288449315578152	25.094757158137877	25.888663245849287	24.728130280434684
38-39	23.297471592230302	25.695273381183437	27.13794626939712	23.869308757189142
40-41	23.56779109243958	24.818430557923946	27.395864014758086	24.217914334878383
42-43	23.277318740601796	25.07014742586076	26.92459733052227	24.727936503015176
44-45	22.61130574976359	25.088750058133225	27.353814314725533	24.946129877377647
46-47	23.09439285658921	24.57620878354287	27.57142635682949	24.757972003038432
48-49	22.463453578681385	24.614964267443842	28.233370021858097	24.68821213201668
50-51	22.36172043344133	24.565163470631095	27.980684266823758	25.092431829103816
52-53	21.8819275427473	24.53067108995923	28.567442293084472	25.019959074209
54-55	21.24711271644938	25.059489667787993	28.461252267195807	25.23214534856682
56-57	21.13088721163442	25.129685789305405	28.421001637422368	25.318425361637807
58-59	21.427713271427905	24.881020664424017	28.079898305610246	25.611367758537835
60-61	20.877425855771186	24.796581759696156	28.037321603317476	26.288670781215178
62-63	20.608268498512754	24.32260127312011	28.676013215645618	26.39311701272151
64-65	21.56452012959834	24.536290635124868	27.791169950548	26.10801928472879
66-67	21.43996279466336	24.562691961128174	27.29941575994807	26.697929484260396
68-69	21.23397699857573	24.673920415459595	26.879111722587705	27.21299086337697
70-71	21.78958016021579	24.53483550171106	26.747394652497608	26.928189685575543
72-73	21.777869068473187	23.92860464755763	26.770156727176897	27.52336955679229
74-75	21.418799510130686	24.3182910381819	26.839141488520625	27.42376796316679
76	21.870882229835523	24.289999534934193	26.581611297998663	27.257506937231618
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	2.5
12	5.0
13	4.0
14	4.5
15	11.0
16	19.5
17	23.0
18	37.0
19	51.5
20	70.0
21	88.0
22	128.5
23	262.0
24	440.5
25	526.0
26	670.5
27	955.0
28	1274.0
29	1453.0
30	1714.0
31	2266.5
32	3242.5
33	3927.0
34	4412.0
35	5525.5
36	6899.0
37	7644.0
38	8545.5
39	10799.5
40	13186.5
41	14887.0
42	15553.0
43	15891.5
44	16499.5
45	16906.0
46	17043.0
47	16583.5
48	15217.0
49	13581.0
50	12852.0
51	11968.0
52	9996.5
53	8095.5
54	7282.0
55	6632.0
56	5536.5
57	4494.0
58	3897.0
59	3577.0
60	3061.0
61	2801.0
62	2737.0
63	2670.0
64	2521.0
65	2445.5
66	2409.0
67	2366.0
68	2458.0
69	2533.0
70	2422.5
71	2329.0
72	2321.0
73	2245.5
74	2074.0
75	1970.0
76	1764.5
77	1450.5
78	1259.0
79	1176.0
80	1024.0
81	763.0
82	555.0
83	456.0
84	357.5
85	213.0
86	123.5
87	80.0
88	69.0
89	41.5
90	22.0
91	14.0
92	9.0
93	6.0
94	4.0
95	3.5
96	2.0
97	2.5
98	2.0
99	3.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	1.9377741950485993E-4
58-59	0.0
60-61	1.9377741950485993E-4
62-63	1.9377741950485993E-4
64-65	0.0
66-67	1.9377741950485993E-4
68-69	1.9377741950485993E-4
70-71	3.8755483900971985E-4
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	258028.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.23864924373361
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.45063812498479	28.490238450272386
2	14.616970714770128	12.348021980542033
3	6.422513788115292	8.13834921477734
4	2.92613065387872	4.943832253220806
5	1.6996850099371752	3.589619947978412
6	1.0175509506698344	2.5787986615782494
7	0.8191113723526546	2.421871056384994
8	0.6019233402547024	2.033954307250792
9	0.5235812089934636	1.9903826733556407
>10	3.794966260918319	30.008154948757287
>50	0.12395368664544397	3.3164614532381176
>100	0.0029748884794906553	0.14031505264394661
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0019377741950485994	0.0
2	0.0	0.0	0.0	0.0019377741950485994	0.0
3	0.0	0.0	0.0	0.0019377741950485994	0.0
4	0.0	0.0	0.0	0.003100438712077759	0.0
5	0.0	0.0	0.0	0.003100438712077759	0.0
6	0.0	0.0	0.0	0.003100438712077759	0.0
7	0.0	0.0	0.0	0.003100438712077759	0.0
8	0.0	0.0	0.0	0.003100438712077759	0.0
9	0.0	0.0	0.0	0.003875548390097199	0.0
10	0.0	0.0	0.0	0.004263103229106919	0.0
11	0.0	0.0	0.0	0.004263103229106919	0.0
12	0.0	0.0	0.0	0.004263103229106919	0.0
13	0.0	0.0	0.0	0.004263103229106919	0.0
14	0.0	0.0	0.0	0.004263103229106919	0.0
15	0.0	0.0	0.0	0.004263103229106919	0.0
16	0.0	0.0	0.0	0.004263103229106919	0.0
17	0.0	0.0	0.0	0.004263103229106919	0.0
18	0.0	0.0	0.0	0.005038212907126359	0.0
19	0.0	0.0	0.0	0.005813322585145798	0.0
20	0.0	0.0	0.0	0.005813322585145798	0.0
21	0.0	0.0	0.0	0.006588432263165238	0.0
22	0.0	0.0	0.0	0.008138651619204117	0.0
23	0.0	0.0	0.0	0.009301316136233277	0.0
24	0.0	0.0	0.0	0.009688870975242996	0.0
25	0.0	0.0	0.0	0.009688870975242996	0.0
26	0.0	0.0	0.0	0.010076425814252717	0.0
27	0.0	0.0	0.0	0.021703070984544312	0.0
28	0.0	0.0	0.0	0.03487993551087479	0.0
29	0.0	0.0	0.0	0.05503278713938022	0.0
30	0.0	0.0	0.0	0.0720852000558079	0.0
31	0.0	0.0	0.0	0.0895251678112453	0.0
32	0.0	0.0	0.0	0.11394112266885764	0.0
33	0.0	0.0	0.0	0.1542468259258685	0.0
34	0.0	0.0	0.0	0.22284403243058892	0.0
35	0.0	0.0	0.0	0.3189576325049995	0.0
36	0.0	0.0	0.0	0.5011084068395678	0.0
37	0.0	0.0	0.0	0.7836358844776535	0.0
38	0.0	0.0	0.0	1.1146077169919544	0.0
39	0.0	0.0	0.0	1.4951865688994992	0.0
40	0.0	0.0	0.0	1.9238222208442495	0.0
41	0.0	0.0	0.0	2.3528454276280093	0.0
42	0.0	0.0	0.0	2.857829382857674	0.0
43	0.0	0.0	0.0	3.4562140542886817	0.0
44	0.0	0.0	0.0	4.155362983862217	0.0
45	0.0	0.0	0.0	4.990543661928163	0.0
46	0.0	3.8755483900971985E-4	0.0	5.896646875532888	0.0
47	0.0	3.8755483900971985E-4	0.0	6.832979366580371	0.0
48	0.0	3.8755483900971985E-4	0.0	7.721642612429659	0.0
49	0.0	3.8755483900971985E-4	0.0	8.586665013099353	0.0
50	0.0	3.8755483900971985E-4	0.0	9.330382749159005	0.0
51	0.0	3.8755483900971985E-4	0.0	10.123707504611902	0.0
52	0.0	3.8755483900971985E-4	0.0	10.810842234176135	0.0
53	0.0	3.8755483900971985E-4	0.0	11.561535957337963	0.0
54	0.0	3.8755483900971985E-4	0.0	12.382764661199559	0.0
55	0.0	3.8755483900971985E-4	0.0	13.265614584463702	0.0
56	0.0	3.8755483900971985E-4	0.0	14.162416481932194	0.0
57	0.0	3.8755483900971985E-4	0.0	15.008061140651401	0.0
58	0.0	3.8755483900971985E-4	0.0	15.878896863906244	0.0
59	0.0	3.8755483900971985E-4	0.0	16.646642999984497	0.0
60	0.0	3.8755483900971985E-4	0.0	17.423302897359978	0.0
61	0.0	3.8755483900971985E-4	0.0	18.135628691459843	0.0
62	0.0	3.8755483900971985E-4	0.0	18.861518904925045	0.0
63	0.0	3.8755483900971985E-4	0.0	19.556404731269474	0.0
64	0.0	3.8755483900971985E-4	0.0	20.262917202784195	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCTTG	20	0.0069459593	52.499996	15
GCGTTTC	30	5.870881E-4	46.666668	7
CGTTTCC	35	0.0012539595	40.000004	8
GTACAAG	55	6.679038E-6	38.181816	1
GGTCGGT	60	1.2128994E-5	35.000004	11
CTGTACG	40	0.0024159695	34.999996	41
GACTTTA	40	0.0024159695	34.999996	7
CGGTAAG	40	0.0024159695	34.999996	28
GTCGGGA	40	0.0024159695	34.999996	9
TGTATAG	75	1.4859033E-6	32.666668	3
GCGTATC	75	1.4859033E-6	32.666668	47
CGTATCA	65	2.0977399E-5	32.307693	48
AAAGCGA	55	2.9870702E-4	31.81818	25
GCATAAG	45	0.0043023312	31.111109	60
GTTTATC	45	0.0043023312	31.111109	39
AAGCGGA	45	0.0043023312	31.111109	45
ACATAAG	45	0.0043023312	31.111109	26
TACATGC	60	4.9737113E-4	29.166668	2
TGTCTAA	60	4.9737113E-4	29.166668	13
CCTATGC	60	4.9737113E-4	29.166668	44
>>END_MODULE