FastQCFastQC Report
Wed 25 May 2016
SRR1781196_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781196_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences232272
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT52442.2576978714610454No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG5400.2324860508369498No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC3670.15800440862437143No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCACCTCGTATGCCGT3190.13733898188330923No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGTAC150.002222663770.07
ATATCGT150.002222663770.037
CAACTCG550.063.6363636
CGTATGC8850.058.1355941
TCGTATG8750.058.040
TATGCCG8750.057.643
CTCGTAT8450.057.5739639
CCGTCTT8700.056.7241447
TCTCGTA7500.056.038
ATCTCGT7600.055.26315737
TCTTCTG8750.055.250
CGTCTTC8900.055.05618348
CTGCTTG8700.054.71264654
GTATGCC9300.054.56989742
GCCGTCT9050.054.53038446
CTTGAAA8950.053.96648457
CATCTCG7800.053.84615336
CTTCTGC8950.053.5754251
GTCTTCT9100.053.4615449
TTGAAAA9250.053.3513558
Produced by FastQC (version 0.11.5)