##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781196_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	232272
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.30927102707171	34.0	34.0	34.0	33.0	34.0
2	33.31387338981883	34.0	34.0	34.0	33.0	34.0
3	33.363268065027206	34.0	34.0	34.0	33.0	34.0
4	33.43078373630915	34.0	34.0	34.0	34.0	34.0
5	33.43419353172143	34.0	34.0	34.0	34.0	34.0
6	36.86852483295447	38.0	37.0	38.0	34.0	38.0
7	36.773196941516844	38.0	37.0	38.0	34.0	38.0
8	36.555572776744505	38.0	37.0	38.0	34.0	38.0
9	36.51861179995867	38.0	37.0	38.0	34.0	38.0
10-11	36.45188830336846	38.0	37.0	38.0	34.0	38.0
12-13	36.38212096163119	38.0	37.0	38.0	34.0	38.0
14-15	36.389233312667905	38.0	37.0	38.0	34.0	38.0
16-17	36.316441499621135	38.0	37.0	38.0	34.0	38.0
18-19	36.3095551766894	38.0	37.0	38.0	34.0	38.0
20-21	36.24484010126059	38.0	37.0	38.0	34.0	38.0
22-23	36.31784072122339	38.0	37.0	38.0	34.0	38.0
24-25	36.31098023007509	38.0	37.0	38.0	34.0	38.0
26-27	36.09997976510299	38.0	37.0	38.0	33.0	38.0
28-29	36.082560532479164	38.0	37.0	38.0	32.5	38.0
30-31	36.085884221946685	38.0	37.0	38.0	33.0	38.0
32-33	36.07053368464559	38.0	37.0	38.0	32.5	38.0
34-35	36.04936023283047	38.0	37.0	38.0	32.5	38.0
36-37	36.0012549941448	38.0	37.0	38.0	32.0	38.0
38-39	35.79893185575532	38.0	36.0	38.0	31.0	38.0
40-41	35.91613065716057	38.0	36.5	38.0	32.0	38.0
42-43	35.99172306606048	38.0	37.0	38.0	32.0	38.0
44-45	35.97071106289178	38.0	37.0	38.0	32.0	38.0
46-47	35.97251928773163	38.0	37.0	38.0	32.0	38.0
48-49	35.943346593648826	38.0	37.0	38.0	32.0	38.0
50-51	35.83919930081973	38.0	36.0	38.0	31.0	38.0
52-53	35.87494833643315	38.0	37.0	38.0	32.0	38.0
54-55	35.901329045257285	38.0	37.0	38.0	32.0	38.0
56-57	35.84781420059241	38.0	36.5	38.0	31.5	38.0
58-59	35.818508042295235	38.0	36.0	38.0	31.0	38.0
60-61	35.82310825239375	38.0	36.0	38.0	31.5	38.0
62-63	35.79427782944134	38.0	36.0	38.0	31.0	38.0
64-65	35.76155757043466	38.0	36.0	38.0	31.0	38.0
66-67	35.7183625232486	38.0	36.0	38.0	31.0	38.0
68-69	35.595777794998966	38.0	36.0	38.0	31.0	38.0
70-71	35.38625619962802	38.0	36.0	38.0	29.5	38.0
72-73	35.20920946132121	38.0	36.0	38.0	27.0	38.0
74-75	35.215639422745745	38.0	36.0	38.0	27.0	38.0
76	34.335882069298066	37.0	34.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	11.0
22	29.0
23	72.0
24	159.0
25	353.0
26	636.0
27	1186.0
28	2036.0
29	3239.0
30	4770.0
31	7172.0
32	10048.0
33	13731.0
34	18883.0
35	26481.0
36	38907.0
37	104556.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.6867121306055	18.223031618102915	10.019718261348762	28.070537989942824
2	22.81032582489495	19.721705586553696	32.80205965419852	24.665908934352828
3	19.582644485775297	21.99188882000413	30.258490046152787	28.16697664806778
4	14.124388647792244	18.825773231383895	35.81103189364194	31.238806227181925
5	13.637459530205964	33.73889233312668	37.10735689191982	15.516291244747539
6	29.718175242818763	39.39433078459737	16.81562995109182	14.071864021492045
7	28.38482468829648	28.297427154370737	21.564372804298408	21.753375353034375
8	24.923796238892333	36.145984018736655	18.639784390714333	20.290435351656676
9	24.038196597093066	15.614452021767583	20.221550595853138	40.125800785286216
10-11	25.176517186746572	25.272740580009646	27.30139663842392	22.249345594819868
12-13	24.7451264035269	22.302731280567613	26.45454467176414	26.49759764414135
14-15	23.18402562512916	22.884592202245642	22.639836054281187	31.29154611834401
16-17	24.97029344905972	26.92812736791348	23.9712492250465	24.130329957980297
18-19	24.682914858441826	25.597359991733832	24.835107115795275	24.884618034029067
20-21	22.875120548322656	25.704777157814974	24.682269063856168	26.737833230006196
22-23	23.474633188675345	26.596188950885168	24.772680305848315	25.156497554591166
24-25	24.639000826617067	26.751179651443135	24.88354170971964	23.726277812220157
26-27	24.05864675897224	27.092374457532546	25.06436419370393	23.78461458979128
28-29	24.478413239650067	25.384893573052285	25.77409244334229	24.362600743955362
30-31	23.561384928015432	27.998639526072882	25.138200041330855	23.301775504580835
32-33	22.483769029413793	27.506974581525107	26.36219604601502	23.647060343046082
34-35	25.169843976028105	24.977181924640078	25.2903922986843	24.562581800647518
36-37	23.60723634359716	25.994738926775508	24.693247571812357	25.704777157814974
38-39	22.629933870634428	25.89098126334642	26.80779431011917	24.67129055589998
40-41	22.744670042019703	24.241191361851623	27.835038231039473	25.17910036508921
42-43	24.17682716814769	24.59250361644968	27.137364813666736	24.093304401735896
44-45	22.072828408073295	26.30235241441069	27.59178893710822	24.0330302404078
46-47	23.91269718261349	24.12150409864297	26.50534717916925	25.46045153957429
48-49	23.199094165461183	23.923675690569677	26.999595302059653	25.877634841909487
50-51	21.652631397671694	23.988255149135497	28.3891299855342	25.969983467658608
52-53	21.003392574223327	23.941112144382448	30.29723772129228	24.75825756010195
54-55	20.34941792381346	24.37530137080664	29.133515877936212	26.141764827443687
56-57	21.496564372804297	24.46872632086519	27.51902941379073	26.51567989253978
58-59	20.382999242267687	24.45688675346146	29.70095405386788	25.459159950402977
60-61	21.727113039884273	25.346361162774677	26.996151064269476	25.93037473307157
62-63	19.3122718192464	27.23660191499621	26.988616794103464	26.462509471653924
64-65	19.831490666115588	27.69447027622787	26.052645174622857	26.421393883033684
66-67	20.111550251429357	27.598462147826687	25.366380794930084	26.923606805813872
68-69	20.064191981814425	28.180538334366606	24.832739202314528	26.92253048150444
70-71	20.685446373217605	27.162550802507408	24.754598057449886	27.3974047668251
72-73	20.485250051663566	26.07288007164015	24.751799614245368	28.690070262450917
74-75	20.700299648687746	26.239925604463732	24.867612109940072	28.192162636908453
76	21.135134669697596	26.148653303024037	24.716280912034165	27.9999311152442
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	0.5
11	0.5
12	1.0
13	1.0
14	6.0
15	13.0
16	22.0
17	29.0
18	42.0
19	60.5
20	86.5
21	107.0
22	143.0
23	237.5
24	399.0
25	502.0
26	613.0
27	786.5
28	1039.5
29	1230.0
30	1448.5
31	1856.5
32	2779.5
33	3513.0
34	3833.5
35	4732.5
36	5912.5
37	6514.0
38	7377.5
39	9156.5
40	11615.5
41	14212.5
42	15266.0
43	15727.0
44	16147.5
45	15837.5
46	15568.0
47	15105.0
48	13519.5
49	11810.5
50	11224.0
51	10426.0
52	8738.0
53	7065.5
54	6283.0
55	5784.0
56	4740.5
57	3812.0
58	3428.0
59	3070.5
60	2601.5
61	2382.0
62	2274.0
63	2204.5
64	2154.5
65	2220.5
66	2262.0
67	2257.0
68	2276.5
69	2277.0
70	2264.5
71	2271.0
72	2170.5
73	2024.0
74	1888.0
75	1798.0
76	1706.5
77	1507.0
78	1238.5
79	1078.0
80	971.5
81	726.5
82	492.0
83	396.0
84	312.0
85	188.5
86	109.5
87	70.0
88	62.5
89	42.0
90	19.5
91	7.0
92	4.0
93	3.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	232272.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.48555142247021
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.773922327505666	20.50139491630502
2	19.339262099171346	13.725287593855478
3	8.328985841330697	8.866759661087
4	3.8520801232665636	5.467727491906041
5	2.1535251082828823	3.820951298477647
6	1.30303434720891	2.774333539987601
7	0.9851619086905353	2.4471309499207825
8	0.789828081967412	2.242198801405249
9	0.6503039200223238	2.0768753874767514
>10	4.639481698069713	30.971877798443202
>50	0.16864224791623697	3.820090239030103
>100	0.013345789403443215	0.7951883998071226
>500	0.0012132535821312012	0.2324860508369498
>1k	0.0	0.0
>5k	0.0012132535821312012	2.2576978714610454
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT	5244	2.2576978714610454	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG	540	0.2324860508369498	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC	367	0.15800440862437143	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCACCTCGTATGCCGT	319	0.13733898188330923	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.002152648618860646	0.0
2	0.0	0.0	0.0	0.002152648618860646	0.0
3	0.0	0.0	0.0	0.002152648618860646	0.0
4	0.0	0.0	0.0	0.004305297237721292	0.0
5	0.0	0.0	0.0	0.004305297237721292	0.0
6	0.0	0.0	0.0	0.004305297237721292	0.0
7	0.0	0.0	0.0	0.004305297237721292	0.0
8	0.0	0.0	0.0	0.004305297237721292	0.0
9	0.0	0.0	0.0	0.0051663566852655505	0.0
10	0.0	0.0	0.0	0.006027416132809809	0.0
11	0.0	0.0	0.0	0.006027416132809809	0.0
12	0.0	0.0	0.0	0.006027416132809809	0.0
13	0.0	0.0	0.0	0.006027416132809809	0.0
14	0.0	0.0	0.0	0.006027416132809809	0.0
15	0.0	0.0	0.0	0.006027416132809809	0.0
16	0.0	0.0	0.0	0.006457945856581939	0.0
17	0.0	0.0	0.0	0.006457945856581939	0.0
18	0.0	0.0	0.0	0.007319005304126197	0.0
19	0.0	0.0	0.0	0.008180064751670456	0.0
20	0.0	0.0	0.0	0.008180064751670456	0.0
21	0.0	0.0	0.0	0.008610594475442584	0.0
22	0.0	0.0	0.0	0.009471653922986843	0.0
23	0.0	0.0	0.0	0.011624302541847488	0.0
24	0.0	0.0	0.0	0.012054832265619618	0.0
25	0.0	0.0	0.0	0.012485361989391748	0.0
26	0.0	0.0	0.0	0.013346421436936005	0.0
27	0.0	0.0	0.0	0.024540194255011367	0.0
28	0.0	0.0	0.0	0.038317145415719504	0.0
29	0.0	0.0	0.0	0.059843631604325966	0.0
30	0.0	0.0	0.0	0.07921746917407178	0.0
31	0.0	0.0	0.0	0.1003134256389061	0.0
32	0.0	0.0	0.0	0.12571467934146174	0.0
33	0.0	0.0	0.0	0.16962871116621892	0.0
34	0.0	0.0	0.0	0.24497141282634152	0.0
35	0.0	0.0	0.0	0.3526038437693738	0.0
36	0.0	0.0	0.0	0.5571054625611352	0.0
37	0.0	0.0	0.0	0.8718226906385617	0.0
38	0.0	0.0	0.0	1.2218433560653028	0.0
39	0.0	0.0	0.0	1.629124474753737	0.0
40	0.0	8.610594475442584E-4	0.0	2.0811806847144725	0.0
41	0.0	8.610594475442584E-4	0.0	2.5409864297031066	0.0
42	0.0	8.610594475442584E-4	0.0	3.0800096438658127	0.0
43	0.0	8.610594475442584E-4	0.0	3.7163325756010197	0.0
44	0.0	8.610594475442584E-4	0.0	4.453829992422677	0.0
45	0.0	8.610594475442584E-4	0.0	5.322208445271062	0.0
46	0.0	8.610594475442584E-4	0.0	6.271526486188606	0.0
47	0.0	8.610594475442584E-4	0.0	7.248398429427568	0.0
48	0.0	8.610594475442584E-4	0.0	8.196424881173796	0.0
49	0.0	8.610594475442584E-4	0.0	9.105703657780532	0.0
50	0.0	8.610594475442584E-4	0.0	9.895725700902391	0.0
51	0.0	8.610594475442584E-4	0.0	10.718898532754702	0.0
52	0.0	8.610594475442584E-4	0.0	11.445632706482055	0.0
53	0.0	8.610594475442584E-4	0.0	12.240821106289179	0.0
54	0.0	8.610594475442584E-4	0.0	13.103602672728526	0.0
55	0.0	8.610594475442584E-4	0.0	14.026227870772198	0.0
56	0.0	8.610594475442584E-4	0.0	14.957033133567542	0.0
57	0.0	8.610594475442584E-4	0.0	15.8891299855342	0.0
58	0.0	8.610594475442584E-4	0.0	16.783340221808913	0.0
59	0.0	8.610594475442584E-4	0.0	17.62588689123097	0.0
60	0.0	8.610594475442584E-4	0.0	18.44303230695047	0.0
61	0.0	8.610594475442584E-4	0.0	19.185696080457394	0.0
62	0.0	8.610594475442584E-4	0.0	19.939984156506164	0.0
63	0.0	8.610594475442584E-4	0.0	20.668009919404835	0.0
64	0.0	8.610594475442584E-4	0.0	21.401632568712543	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGGTAC	15	0.0022226637	70.0	7
ATATCGT	15	0.0022226637	70.0	37
CAACTCG	55	0.0	63.63636	36
CGTATGC	885	0.0	58.13559	41
TCGTATG	875	0.0	58.0	40
TATGCCG	875	0.0	57.6	43
CTCGTAT	845	0.0	57.57396	39
CCGTCTT	870	0.0	56.72414	47
TCTCGTA	750	0.0	56.0	38
ATCTCGT	760	0.0	55.263157	37
TCTTCTG	875	0.0	55.2	50
CGTCTTC	890	0.0	55.056183	48
CTGCTTG	870	0.0	54.712646	54
GTATGCC	930	0.0	54.569897	42
GCCGTCT	905	0.0	54.530384	46
CTTGAAA	895	0.0	53.966484	57
CATCTCG	780	0.0	53.846153	36
CTTCTGC	895	0.0	53.57542	51
GTCTTCT	910	0.0	53.46154	49
TTGAAAA	925	0.0	53.35135	58
>>END_MODULE