FastQCFastQC Report
Wed 25 May 2016
SRR1781195_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781195_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences231859
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATG200.006945074552.519
GATCATA200.006945074552.51
ACAGTCG305.8696436E-446.66666441
TACCACG305.8696436E-446.66666420
ATCACGT305.8696436E-446.66666439
ATCGTAC305.8696436E-446.66666425
TACGAAC305.8696436E-446.66666428
CATCGTA305.8696436E-446.66666424
ACGAACG404.58341E-543.7529
CACGCTA350.001253696740.023
CCGAAAT350.001253696740.047
AACGCAC459.179189E-538.8888932
TACATGC400.002415465435.02
TACGTTG400.002415465435.046
GACTATC501.7062832E-435.038
GTATAGA652.096829E-532.3076931
CGTATTG552.9861426E-431.8181843
CGCACTC552.9861426E-431.8181834
CTACTGA450.00430143931.11111333
ACTGATC450.00430143931.11111324