##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781195_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	231859
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32444287260792	34.0	33.0	34.0	23.0	34.0
2	31.40107565373783	34.0	33.0	34.0	23.0	34.0
3	31.35599221940921	34.0	33.0	34.0	23.0	34.0
4	31.52636731806831	34.0	33.0	34.0	23.0	34.0
5	31.478156983339012	34.0	33.0	34.0	23.0	34.0
6	34.234431270729196	37.0	34.0	38.0	24.0	38.0
7	34.16581198055715	37.0	34.0	38.0	24.0	38.0
8	34.112516658831446	37.0	34.0	38.0	24.0	38.0
9	34.3727135888622	37.0	34.0	38.0	25.0	38.0
10-11	34.23295192336722	37.5	34.0	38.0	24.0	38.0
12-13	34.186477557481055	37.0	34.0	38.0	24.0	38.0
14-15	34.066165212478275	37.0	34.0	38.0	23.5	38.0
16-17	34.025560793413234	37.0	34.0	38.0	23.5	38.0
18-19	34.08069775165079	37.0	34.0	38.0	23.5	38.0
20-21	34.0130445658784	37.0	34.0	38.0	23.5	38.0
22-23	34.07738970667518	37.0	34.0	38.0	23.5	38.0
24-25	34.07780375141789	37.0	34.0	38.0	23.5	38.0
26-27	33.98755924937139	37.0	34.0	38.0	23.0	38.0
28-29	34.08152799761925	37.0	34.0	38.0	24.0	38.0
30-31	34.151954420574576	37.0	34.0	38.0	24.0	38.0
32-33	34.092273752582386	37.0	34.0	38.0	23.5	38.0
34-35	34.1006107159955	37.0	34.0	38.0	24.0	38.0
36-37	33.96424335479753	37.0	34.0	38.0	23.0	38.0
38-39	33.972187838298275	37.0	34.0	38.0	23.0	38.0
40-41	33.97735908461608	37.0	34.0	38.0	23.5	38.0
42-43	34.03584074804083	37.0	34.0	38.0	23.5	38.0
44-45	34.074258493308434	37.0	34.0	38.0	23.5	38.0
46-47	34.06252291263225	37.0	34.0	38.0	23.5	38.0
48-49	34.03600464075149	37.0	34.0	38.0	23.5	38.0
50-51	33.89579227030221	37.0	34.0	38.0	23.0	38.0
52-53	33.990852198965754	37.0	34.0	38.0	23.5	38.0
54-55	33.9587313841602	37.0	34.0	38.0	23.0	38.0
56-57	33.93308001845949	37.0	34.0	38.0	23.0	38.0
58-59	33.90808206711838	37.0	34.0	38.0	23.0	38.0
60-61	33.89508063090068	37.0	34.0	38.0	23.0	38.0
62-63	33.8690669760501	37.0	34.0	38.0	23.0	38.0
64-65	33.8812662005788	37.0	34.0	38.0	23.0	38.0
66-67	33.79147024700357	37.0	34.0	38.0	23.0	38.0
68-69	33.55615697471308	37.0	34.0	38.0	22.0	38.0
70-71	33.707710289443156	37.0	34.0	38.0	23.0	38.0
72-73	33.6604099905546	37.0	34.0	38.0	22.0	38.0
74-75	33.62696293868257	37.0	34.0	38.0	22.5	38.0
76	32.25848036953493	37.0	31.0	38.0	19.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	5.0
13	24.0
14	104.0
15	298.0
16	777.0
17	1365.0
18	1885.0
19	2077.0
20	2024.0
21	2005.0
22	2081.0
23	2315.0
24	2721.0
25	3219.0
26	3821.0
27	4496.0
28	5324.0
29	6259.0
30	7607.0
31	8984.0
32	10879.0
33	13403.0
34	17057.0
35	22804.0
36	34630.0
37	75693.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.91436605868221	19.38290081471929	9.948287536821947	25.754445589776548
2	22.858288873841428	19.74389607476958	32.16049409339297	25.237320957996022
3	19.6891214056819	22.036237540919267	29.26735645370678	29.007284599692056
4	13.877830923104128	16.076149728930083	37.42317529187998	32.62284405608581
5	13.927430032907932	34.33552288244148	35.50649317041823	16.230553914232356
6	30.696242112663302	36.408334375633466	17.927706062736405	14.96771744896683
7	29.325581495650372	28.393118231338875	22.889773526151668	19.391526746859082
8	26.283646526552772	33.29221639013366	19.696453448000725	20.72768363531284
9	25.001401713972715	15.100125507312633	21.612272976248494	38.28619980246615
10-11	25.77493217860855	24.205875122380412	27.41191845043755	22.607274248573486
12-13	25.528230519410506	22.655795116859814	26.47902388951906	25.336950474210617
14-15	23.55246076279118	23.94774410309714	23.8457424555441	28.654052678567577
16-17	24.005106551826756	26.508999003704837	25.229988915677197	24.255905528791207
18-19	23.432129009441084	26.163961718113164	26.424896165341867	23.979013107103885
20-21	23.34155672197327	26.398155775708513	26.328070077072702	23.93221742524552
22-23	23.761855265484627	25.749916975403153	26.64399484169258	23.84423291741964
24-25	23.566693550821835	26.028103286911442	26.306073950116232	24.099129212150487
26-27	22.82809811135216	26.27803967066191	26.876679361163465	24.017182856822465
28-29	23.395468797846966	25.969446948360858	27.348733497513578	23.2863507562786
30-31	23.518819627445993	25.88297197865944	26.671166527932925	23.92704186596164
32-33	22.919532992033954	26.292919403603054	26.60345296063556	24.184094643727438
34-35	23.876795811247355	25.454047503008294	26.94288339033637	23.726273295407985
36-37	23.919494175339324	25.58343648510517	26.28580300958772	24.21126632996778
38-39	23.317619760285346	25.929767660517815	26.990541665408717	23.762070913788122
40-41	23.494667017454574	25.124536895268246	27.553383737530133	23.827412349747046
42-43	23.271471023337458	25.284547936461383	27.056530046278127	24.387450993923032
44-45	22.398957987397512	25.16033451364838	27.509391483617197	24.931316015336908
46-47	22.639837142401202	24.90392868079307	27.81819985422175	24.638034322583984
48-49	22.22040119210382	24.912770261236357	28.38255146446763	24.484277082192193
50-51	22.173389861941956	25.052510361900982	27.985542937733708	24.788556838423354
52-53	21.841938419470456	24.97681780737431	28.486709595055615	24.69453417809962
54-55	21.17191914051212	25.434207859086772	28.48067144255776	24.913201557843344
56-57	20.906887375517016	25.569850641985	28.445520769088112	25.077741213409876
58-59	21.043392751629224	25.287782661013807	28.184155025252416	25.484669562104557
60-61	21.07509305224296	24.995147913171365	28.32411077422054	25.60564826036514
62-63	20.57241685679659	24.86403374464653	28.67928353007647	25.884265868480412
64-65	21.483746587365594	25.072134357519012	27.74660461746147	25.69751443765392
66-67	21.49474465084383	24.810768613683315	27.315954955382367	26.378531780090487
68-69	21.183779797204334	25.065233611807177	26.887030479731216	26.86395611125727
70-71	21.79708357234354	25.042159243333234	26.953450157207616	26.20730702711562
72-73	21.723763149155307	24.44093177318974	26.829883679305095	27.00542139834986
74-75	21.576259709564866	25.112460590272534	26.628036867233966	26.683242832928634
76	21.591139442506005	24.921611841679642	26.538542821283624	26.94870589453073
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	1.0
9	2.0
10	2.0
11	1.0
12	1.0
13	3.5
14	9.5
15	12.5
16	22.0
17	32.0
18	44.0
19	79.5
20	127.0
21	151.0
22	191.0
23	290.0
24	517.5
25	686.0
26	804.0
27	1032.5
28	1373.5
29	1604.0
30	1879.0
31	2409.5
32	3273.5
33	3882.0
34	4266.0
35	5228.5
36	6441.5
37	7076.0
38	7982.5
39	9752.0
40	11451.0
41	12959.5
42	13632.0
43	13848.0
44	14342.5
45	14802.5
46	14984.0
47	14646.5
48	13509.5
49	11905.0
50	11100.0
51	10550.0
52	9024.0
53	7563.0
54	7078.0
55	6533.5
56	5418.5
57	4517.5
58	4187.0
59	3729.0
60	3101.0
61	2728.5
62	2526.0
63	2365.5
64	2180.0
65	2191.5
66	2118.0
67	2008.0
68	1941.0
69	1898.0
70	1868.0
71	1814.0
72	1697.5
73	1545.0
74	1470.5
75	1432.0
76	1383.5
77	1193.5
78	964.5
79	877.0
80	773.0
81	579.5
82	434.5
83	379.0
84	316.0
85	204.0
86	136.0
87	117.0
88	90.0
89	53.0
90	33.5
91	19.0
92	14.0
93	11.0
94	5.0
95	3.5
96	5.0
97	4.0
98	2.0
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	231859.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.168173192198054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.44444251224611	31.59954875948821
2	15.421883583279259	14.240003844455055
3	6.1516743144892	8.520346955200008
4	2.7455919369629513	5.070358562432322
5	1.399104194727138	3.229704238804665
6	0.9163596176186839	2.5383989719533466
7	0.6914820449659114	2.234712396789703
8	0.5780567163361132	2.135025807577518
9	0.463638961204428	1.9264827473583144
>10	3.1031967926059916	26.01256595605625
>50	0.08257945908037774	2.3913370668802876
>100	0.0019898664838645243	0.1015146930043303
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.312966069895928E-4	0.0
2	0.0	0.0	0.0	4.312966069895928E-4	0.0
3	0.0	0.0	0.0	4.312966069895928E-4	0.0
4	0.0	0.0	0.0	4.312966069895928E-4	0.0
5	0.0	0.0	0.0	4.312966069895928E-4	0.0
6	0.0	0.0	0.0	4.312966069895928E-4	0.0
7	0.0	0.0	0.0	4.312966069895928E-4	0.0
8	0.0	0.0	0.0	4.312966069895928E-4	0.0
9	0.0	0.0	0.0	0.0012938898209687785	0.0
10	0.0	0.0	0.0	0.0012938898209687785	0.0
11	0.0	0.0	0.0	0.0012938898209687785	0.0
12	0.0	0.0	0.0	0.002587779641937557	0.0
13	0.0	0.0	0.0	0.002587779641937557	0.0
14	0.0	0.0	0.0	0.002587779641937557	0.0
15	0.0	0.0	0.0	0.002587779641937557	0.0
16	0.0	0.0	0.0	0.002587779641937557	0.0
17	0.0	0.0	0.0	0.002587779641937557	0.0
18	0.0	0.0	0.0	0.002587779641937557	0.0
19	0.0	0.0	0.0	0.002587779641937557	0.0
20	0.0	0.0	0.0	0.002587779641937557	0.0
21	0.0	0.0	0.0	0.003881669462906335	0.0
22	0.0	0.0	0.0	0.006900745711833485	0.0
23	0.0	0.0	0.0	0.006900745711833485	0.0
24	0.0	0.0	0.0	0.008194635532802263	0.0
25	0.0	0.0	0.0	0.008625932139791856	0.0
26	0.0	0.0	0.0	0.00905722874678145	0.0
27	0.0	0.0	0.0	0.017683160886573304	0.0
28	0.0	0.0	0.0	0.02932816927529231	0.0
29	0.0	0.0	0.0	0.04485484712691765	0.0
30	0.0	0.0	0.0	0.060812821585532584	0.0
31	0.0	0.0	0.0	0.07461431300919956	0.0
32	0.0	0.0	0.0	0.09833562639362717	0.0
33	0.0	0.0	0.0	0.1371523210226905	0.0
34	0.0	0.0	0.0	0.1919269901103688	0.0
35	0.0	0.0	0.0	0.28465576061313125	0.0
36	0.0	0.0	0.0	0.4351782764524991	0.0
37	0.0	0.0	0.0	0.6616089951220354	0.0
38	0.0	0.0	0.0	0.9544593912679689	0.0
39	0.0	0.0	0.0	1.3020844565015808	0.0
40	0.0	0.0	0.0	1.6919765892201726	0.0
41	0.0	0.0	0.0	2.101277069253296	0.0
42	0.0	0.0	0.0	2.6494550567370685	0.0
43	0.0	0.0	0.0	3.2670717979461656	0.0
44	0.0	0.0	0.0	3.9524021064526287	0.0
45	0.0	0.0	0.0	4.745987863313479	0.0
46	0.0	0.0	0.0	5.556825484453914	0.0
47	0.0	0.0	0.0	6.378445520769088	0.0
48	0.0	0.0	0.0	7.150897743887449	0.0
49	0.0	0.0	0.0	7.886258458804704	0.0
50	0.0	0.0	0.0	8.541829301428885	0.0
51	0.0	0.0	0.0	9.220258864223515	0.0
52	0.0	0.0	0.0	9.92456622343752	0.0
53	0.0	0.0	0.0	10.617228574262807	0.0
54	0.0	0.0	0.0	11.401325805769886	0.0
55	0.0	0.0	0.0	12.245373265648519	0.0
56	0.0	0.0	0.0	13.051035327505078	0.0
57	0.0	0.0	0.0	13.760518246002958	0.0
58	0.0	0.0	0.0	14.49027210502935	0.0
59	0.0	0.0	0.0	15.172583337286886	0.0
60	0.0	0.0	0.0	15.830741959553004	0.0
61	0.0	0.0	0.0	16.442320548264245	0.0
62	0.0	0.0	0.0	17.143177534622335	0.0
63	0.0	0.0	0.0	17.85783601240409	0.0
64	0.0	0.0	0.0	18.53497168537775	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCATG	20	0.0069450745	52.5	19
GATCATA	20	0.0069450745	52.5	1
ACAGTCG	30	5.8696436E-4	46.666664	41
TACCACG	30	5.8696436E-4	46.666664	20
ATCACGT	30	5.8696436E-4	46.666664	39
ATCGTAC	30	5.8696436E-4	46.666664	25
TACGAAC	30	5.8696436E-4	46.666664	28
CATCGTA	30	5.8696436E-4	46.666664	24
ACGAACG	40	4.58341E-5	43.75	29
CACGCTA	35	0.0012536967	40.0	23
CCGAAAT	35	0.0012536967	40.0	47
AACGCAC	45	9.179189E-5	38.88889	32
TACATGC	40	0.0024154654	35.0	2
TACGTTG	40	0.0024154654	35.0	46
GACTATC	50	1.7062832E-4	35.0	38
GTATAGA	65	2.096829E-5	32.307693	1
CGTATTG	55	2.9861426E-4	31.81818	43
CGCACTC	55	2.9861426E-4	31.81818	34
CTACTGA	45	0.004301439	31.111113	33
ACTGATC	45	0.004301439	31.111113	24
>>END_MODULE