FastQCFastQC Report
Wed 25 May 2016
SRR1781195_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781195_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208820
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT43332.074992816780002No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG4650.22267981994061872No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC2700.12929795996552054No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTACCTCGTATGCCGT2310.11062158797050092No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT6350.058.42519447
CGTCTTC6400.057.9687548
TCGTATG6350.057.87401640
TATGCCG6550.057.70992343
CGTATGC6550.057.1755741
CTTGAAA6200.057.0161357
GCCGTCT6500.056.53846446
CTCGTAT6450.056.43410539
TCTCGTA5650.056.3716838
ATGCCGT6650.056.31579244
CCGATAC252.3864926E-455.99999644
TGCCGTC6700.055.89552345
GTCTTCT6500.055.4615449
ATCTCGT5650.055.13274437
TCTTCTG6500.054.92307750
GTATGCC6900.054.7826142
GCTTGAA6700.053.80597356
TGCTTGA6650.053.15789455
ATTATCT5850.052.64957434
TTGAAAA6650.052.6315858