FastQCFastQC Report
Wed 25 May 2016
SRR1781194_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781194_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113312
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCGC200.00693595552.566
AATGCGT200.00693595552.56
AAATGCG200.00693595552.55
CGTAGGG350.001250984440.00000410
GCGATGT350.001250984440.00000419
GCCTACG350.001250984440.0000041
GCGCGAT400.002410271735.017
AACACGA400.002410271735.026
TCTATAC552.9765745E-431.818183
TTACTCC450.00429224631.1111134
CGCGATG450.00429224631.11111318
TATTCAC450.00429224631.11111320
CGATGTA450.00429224631.11111320
ACAAGCC450.00429224631.1111134
AACAGAT604.9563247E-429.16666631
ACACCTC853.9177357E-628.823536
CTATACT853.9177357E-628.823534
CCTACCA500.007183395328.00000227
GGAGTTT500.007183395328.00000268
TAGATGT500.007183395328.0000024