##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781194_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	113312
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.990901228466534	34.0	34.0	34.0	27.0	34.0
2	32.28756883648687	34.0	34.0	34.0	27.0	34.0
3	32.31535936176221	34.0	34.0	34.0	31.0	34.0
4	32.456315306410616	34.0	34.0	34.0	31.0	34.0
5	32.47414219147134	34.0	34.0	34.0	31.0	34.0
6	35.53352689918102	38.0	37.0	38.0	31.0	38.0
7	35.4062853007625	38.0	36.0	38.0	30.0	38.0
8	35.21686140920644	38.0	36.0	38.0	27.0	38.0
9	35.550100607173114	38.0	37.0	38.0	31.0	38.0
10-11	35.479066647839595	38.0	36.5	38.0	31.0	38.0
12-13	35.44790048715052	38.0	37.0	38.0	31.0	38.0
14-15	35.43083698107879	38.0	36.5	38.0	31.0	38.0
16-17	35.408226842699804	38.0	36.5	38.0	30.5	38.0
18-19	35.45185417255013	38.0	36.5	38.0	31.0	38.0
20-21	35.395496505224514	38.0	36.0	38.0	29.5	38.0
22-23	35.435434905393954	38.0	36.5	38.0	31.0	38.0
24-25	35.44722977266309	38.0	36.5	38.0	31.0	38.0
26-27	35.38658306269416	38.0	36.0	38.0	30.0	38.0
28-29	35.459368822366564	38.0	36.5	38.0	31.0	38.0
30-31	35.53493010449026	38.0	37.0	38.0	31.0	38.0
32-33	35.504068412877714	38.0	36.5	38.0	31.0	38.0
34-35	35.516388378988985	38.0	37.0	38.0	31.0	38.0
36-37	35.43243434058176	38.0	36.0	38.0	31.0	38.0
38-39	35.43092964558034	38.0	36.0	38.0	31.0	38.0
40-41	35.43130471618187	38.0	36.0	38.0	31.0	38.0
42-43	35.49844676645016	38.0	37.0	38.0	31.0	38.0
44-45	35.48664307399039	38.0	36.5	38.0	31.0	38.0
46-47	35.458587792996326	38.0	36.5	38.0	31.0	38.0
48-49	35.454166372493646	38.0	36.0	38.0	31.0	38.0
50-51	35.337347324202206	38.0	36.0	38.0	29.5	38.0
52-53	35.42291195989833	38.0	36.0	38.0	31.0	38.0
54-55	35.39700120022593	38.0	36.0	38.0	31.0	38.0
56-57	35.35625088251906	38.0	36.0	38.0	30.0	38.0
58-59	35.36996081615363	38.0	36.0	38.0	30.5	38.0
60-61	35.36868998870376	38.0	36.0	38.0	30.5	38.0
62-63	35.32815588816719	38.0	36.0	38.0	29.0	38.0
64-65	35.338940271109855	38.0	36.0	38.0	29.5	38.0
66-67	35.243544373058455	38.0	36.0	38.0	27.0	38.0
68-69	34.95916584298221	38.0	36.0	38.0	27.0	38.0
70-71	35.162233479243156	38.0	36.0	38.0	27.0	38.0
72-73	35.168146356961316	38.0	36.0	38.0	27.0	38.0
74-75	35.16085233691048	38.0	36.0	38.0	27.0	38.0
76	34.04043702343971	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	10.0
14	55.0
15	107.0
16	214.0
17	351.0
18	402.0
19	478.0
20	475.0
21	508.0
22	528.0
23	685.0
24	712.0
25	880.0
26	1027.0
27	1263.0
28	1445.0
29	1755.0
30	2317.0
31	2870.0
32	3636.0
33	4811.0
34	6846.0
35	10236.0
36	18664.0
37	53035.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.457780288054224	19.339522733691048	9.886861056198812	26.315835922055918
2	21.867939847500708	20.00670714487433	33.45894521321661	24.66640779440836
3	19.252153346512284	22.14328579497317	29.650875458909915	28.953685399604634
4	13.108938153064106	16.151863880259814	37.499117480937585	33.240080485738495
5	13.117763343688225	34.536500988421345	36.55040948884496	15.79532617904547
6	30.202449872917253	37.61649251623835	18.1340016944366	14.047055916407794
7	29.123129059587686	29.49290454673821	22.445989833380402	18.937976560293702
8	25.398898616210108	34.78272380683423	20.317353854843265	19.5010237221124
9	24.51461451567354	16.370728607737927	21.77262778876024	37.3420290878283
10-11	24.79128424173962	25.051627365151084	27.922020615645298	22.235067777463993
12-13	24.711416266591357	23.183775769556622	27.799791725501272	24.30501623835075
14-15	22.618963569613104	24.548150240045185	24.7745163795538	28.058369810787916
16-17	22.746928833662807	27.888926150804856	25.89487432928551	23.469270686246823
18-19	22.11681022310082	27.549156311776336	27.238068342276193	23.095965122846653
20-21	22.40539395650946	27.498852725218864	27.17540948884496	22.920343829426713
22-23	22.439812199943518	26.80033888731997	27.741104207850892	23.018744704885624
24-25	22.2730160971477	27.314847500706012	27.094217735103076	23.31791866704321
26-27	21.705997599548148	27.429133719288334	27.761843405817565	23.10302527534595
28-29	22.14681587122282	27.10039536853996	28.30238633154476	22.45040242869246
30-31	22.320230866986726	27.388979101948603	27.524445778028806	22.766344253035868
32-33	21.824696413442528	27.459139367410334	27.46752329850325	23.248640920643886
34-35	22.558069754306693	26.913742586839877	27.82406099971759	22.704126659135838
36-37	22.43451708556905	27.02229243151652	27.25571872352443	23.28747175939
38-39	21.72938435470206	27.697860773792716	27.778611268003388	22.794143603501833
40-41	22.11681022310082	27.040384072295964	28.108232137814177	22.73457356678904
42-43	21.78630683422762	27.14231502400452	27.983355690482913	23.08802245128495
44-45	21.705997599548148	26.924774075120023	28.054839734538266	23.314388590793563
46-47	21.792043208133297	26.49807610844394	28.395050833097997	23.314829850324767
48-49	21.346812341146567	26.64192671561706	28.83366280711663	23.17759813611974
50-51	21.342841005365713	26.732826179045468	28.395050833097997	23.529281982490822
52-53	21.376817989268567	26.50469500141203	28.768356396498163	23.35013061282124
54-55	20.963357808528666	26.885060717311493	28.671279299632875	23.480302174526972
56-57	20.785971476983903	26.876235526687374	28.74320460321943	23.59458839310929
58-59	20.977919373058455	26.66531347077097	28.57111338604914	23.785653770121435
60-61	21.020721547585428	26.583680457497884	28.369016520756844	24.02658147415984
62-63	20.383101524992938	26.46851171985315	29.050762496469922	24.09762425868399
64-65	20.872458345100252	26.692230302174526	28.43608796950014	23.999223383225075
66-67	20.965564106184694	26.532053092346796	28.06631248234962	24.436070319118894
68-69	20.86539819260096	26.573972747811354	27.824502259248803	24.736126800338887
70-71	21.524639932222538	26.401881530641063	27.882307257836768	24.191171279299635
72-73	21.366669020050832	26.343635272521887	27.812146992375038	24.477548715052244
74-75	21.852937023439704	26.14727478113527	27.400893109291165	24.598895086133858
76	22.045326179045468	25.8410406664784	27.2760166619599	24.837616492516236
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	1.0
10	1.0
11	1.5
12	2.0
13	2.0
14	3.5
15	18.5
16	32.0
17	32.0
18	56.0
19	97.5
20	156.0
21	197.0
22	252.0
23	341.0
24	473.0
25	571.0
26	672.0
27	867.5
28	1120.0
29	1278.0
30	1465.5
31	1818.0
32	2227.5
33	2472.0
34	2765.5
35	3325.5
36	3923.0
37	4254.0
38	4627.5
39	5438.5
40	6115.5
41	6633.5
42	6912.0
43	6841.0
44	6849.5
45	6786.0
46	6643.0
47	6496.0
48	5920.5
49	5326.5
50	5161.0
51	4791.5
52	4023.0
53	3387.0
54	3150.0
55	2934.0
56	2463.0
57	2033.5
58	1859.0
59	1655.0
60	1318.0
61	1140.5
62	1096.0
63	1008.5
64	866.0
65	776.0
66	766.0
67	791.0
68	759.0
69	703.0
70	654.5
71	630.0
72	580.0
73	506.5
74	473.0
75	463.0
76	404.0
77	336.0
78	292.5
79	258.0
80	233.5
81	177.0
82	121.5
83	98.0
84	88.5
85	57.0
86	36.5
87	38.0
88	28.5
89	18.5
90	13.0
91	5.5
92	3.0
93	2.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	113312.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.4710886755154
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.81502021278148	45.241457215475855
2	14.432071919557506	17.454462016379555
3	4.359247529954321	7.908253318271674
4	1.9074444330935043	4.613809658288619
5	1.0974737671662702	3.318271674668173
6	0.7428379620846164	2.6952132166054787
7	0.553115103398958	2.341323072578368
8	0.3925803768187855	1.899181022310082
9	0.3400417390289108	1.8506424738774359
>10	1.352869923089272	12.424985879695
>50	0.0072970330263714776	0.25240045184975995
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	8.825190624117481E-4	0.0
3	0.0	0.0	0.0	8.825190624117481E-4	0.0
4	0.0	0.0	0.0	8.825190624117481E-4	0.0
5	0.0	0.0	0.0	8.825190624117481E-4	0.0
6	0.0	0.0	0.0	8.825190624117481E-4	0.0
7	0.0	0.0	0.0	8.825190624117481E-4	0.0
8	0.0	0.0	0.0	8.825190624117481E-4	0.0
9	0.0	0.0	0.0	8.825190624117481E-4	0.0
10	0.0	0.0	0.0	0.0017650381248234962	0.0
11	0.0	0.0	0.0	0.0017650381248234962	0.0
12	0.0	0.0	0.0	0.0017650381248234962	0.0
13	0.0	0.0	0.0	0.0017650381248234962	0.0
14	0.0	0.0	0.0	0.0017650381248234962	0.0
15	0.0	0.0	0.0	0.0017650381248234962	0.0
16	0.0	0.0	0.0	0.0017650381248234962	0.0
17	0.0	0.0	0.0	0.0017650381248234962	0.0
18	0.0	0.0	0.0	0.0017650381248234962	0.0
19	0.0	0.0	0.0	0.0017650381248234962	0.0
20	0.0	0.0	0.0	0.0017650381248234962	0.0
21	0.0	0.0	0.0	0.0035300762496469924	0.0
22	0.0	0.0	0.0	0.0035300762496469924	0.0
23	0.0	0.0	0.0	0.0035300762496469924	0.0
24	0.0	0.0	0.0	0.0035300762496469924	0.0
25	0.0	0.0	0.0	0.0035300762496469924	0.0
26	0.0	0.0	0.0	0.0052951143744704885	0.0
27	0.0	0.0	0.0	0.00970770968652923	0.0
28	0.0	0.0	0.0	0.013237785936176222	0.0
29	0.0	0.0	0.0	0.01941541937305846	0.0
30	0.0	0.0	0.0	0.02471053374752895	0.0
31	0.0	0.0	0.0	0.03530076249646993	0.0
32	0.0	0.0	0.0	0.04500847218299915	0.0
33	0.0	0.0	0.0	0.06618892968088111	0.0
34	0.0	0.0	0.0	0.09619457780288054	0.0
35	0.0	0.0	0.0	0.13502541654899747	0.0
36	0.0	0.0	0.0	0.20297938435470206	0.0
37	0.0	0.0	0.0	0.3256495340299351	0.0
38	0.0	0.0	0.0	0.45096724089240325	0.0
39	0.0	0.0	0.0	0.6433563964981643	0.0
40	0.0	0.0	0.0	0.8604560858514544	0.0
41	0.0	0.0	0.0	1.1190341711380967	0.0
42	0.0	0.0	0.0	1.3987927139226208	0.0
43	0.0	0.0	0.0	1.71649957639085	0.0
44	0.0	0.0	0.0	2.1242233832250776	0.0
45	0.0	0.0	0.0	2.5654829144309517	0.0
46	0.0	0.0	0.0	3.0632236656311775	0.0
47	0.0	0.0	0.0	3.5397839593335214	0.0
48	0.0	0.0	0.0	4.024286924597571	0.0
49	0.0	0.0	0.0	4.480549279864445	0.0
50	0.0	0.0	0.0	4.905040948884496	0.0
51	0.0	0.0	0.0	5.356008189776899	0.0
52	0.0	0.0	0.0	5.8105055069189495	0.0
53	0.0	0.0	0.0	6.257942671561706	0.0
54	0.0	0.0	0.0	6.744210674950579	0.0
55	0.0	0.0	0.0	7.316965546455804	0.0
56	0.0	0.0	0.0	7.807646145156736	0.0
57	0.0	0.0	0.0	8.300974301044903	0.0
58	0.0	0.0	0.0	8.827838181304717	0.0
59	0.0	0.0	0.0	9.321166337192883	0.0
60	0.0	0.0	0.0	9.795961592770404	0.0
61	0.0	0.0	0.0	10.246046314600395	0.0
62	0.0	0.0	0.0	10.71731149392827	0.0
63	0.0	0.0	0.0	11.196519344817847	0.0
64	0.0	0.0	0.0	11.693377576955662	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTGCGC	20	0.006935955	52.5	66
AATGCGT	20	0.006935955	52.5	6
AAATGCG	20	0.006935955	52.5	5
CGTAGGG	35	0.0012509844	40.000004	10
GCGATGT	35	0.0012509844	40.000004	19
GCCTACG	35	0.0012509844	40.000004	1
GCGCGAT	40	0.0024102717	35.0	17
AACACGA	40	0.0024102717	35.0	26
TCTATAC	55	2.9765745E-4	31.81818	3
TTACTCC	45	0.004292246	31.111113	4
CGCGATG	45	0.004292246	31.111113	18
TATTCAC	45	0.004292246	31.111113	20
CGATGTA	45	0.004292246	31.111113	20
ACAAGCC	45	0.004292246	31.111113	4
AACAGAT	60	4.9563247E-4	29.166666	31
ACACCTC	85	3.9177357E-6	28.82353	6
CTATACT	85	3.9177357E-6	28.82353	4
CCTACCA	50	0.0071833953	28.000002	27
GGAGTTT	50	0.0071833953	28.000002	68
TAGATGT	50	0.0071833953	28.000002	4
>>END_MODULE