FastQCFastQC Report
Wed 25 May 2016
SRR1781194_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781194_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106369
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT5450.5123673250665137No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTTCG150.00221934270.059
GATCTCG700.055.036
CTCGTAT900.054.44444739
GCGAAGT200.0069347952.532
GGTTATC200.0069347952.57
CGTATGC1000.052.49999641
CGTCTTC1000.052.49999648
TATGCCG1000.052.49999643
TCGTATG1000.052.49999640
TCTCGTA750.051.33333638
ATGCCGT1000.048.99999644
ATCTCGT800.048.12537
CCGTCTT1100.047.72727647
GTATGCC1150.045.65217642
TGCCGTC1100.044.54545645
GCCGTCT1200.040.83333246
AGATCTC953.6379788E-1240.52631835
TACAGAT1100.038.1818232
CTTGAAA1200.037.91666457
TCTTCTG1350.036.29629550