##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781194_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	106369
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61307335783922	34.0	34.0	34.0	31.0	34.0
2	32.87100565014243	34.0	34.0	34.0	31.0	34.0
3	32.99437806127725	34.0	34.0	34.0	31.0	34.0
4	33.183436903609135	34.0	34.0	34.0	32.0	34.0
5	33.09728398311538	34.0	34.0	34.0	31.0	34.0
6	36.208886047626656	38.0	37.0	38.0	34.0	38.0
7	36.06495313484192	38.0	36.0	38.0	32.0	38.0
8	35.74976731942577	38.0	36.0	38.0	31.0	38.0
9	35.86739557577866	38.0	36.0	38.0	31.0	38.0
10-11	35.76118511972473	38.0	36.0	38.0	31.0	38.0
12-13	35.73419887373201	38.0	36.0	38.0	31.0	38.0
14-15	35.779498726132616	38.0	36.0	38.0	31.0	38.0
16-17	35.669137624683884	38.0	36.0	38.0	31.0	38.0
18-19	35.68639359211801	38.0	36.0	38.0	31.0	38.0
20-21	35.59635796143613	38.0	36.0	38.0	31.0	38.0
22-23	35.63990448344913	38.0	36.0	38.0	31.0	38.0
24-25	35.66475194840602	38.0	36.0	38.0	31.0	38.0
26-27	35.39121830608542	38.0	36.0	38.0	31.0	38.0
28-29	35.34385018191391	38.0	36.0	38.0	31.0	38.0
30-31	35.38146922505617	38.0	36.0	38.0	31.0	38.0
32-33	35.331826001936655	38.0	36.0	38.0	31.0	38.0
34-35	35.34047513843319	38.0	36.0	38.0	31.0	38.0
36-37	35.285614229709786	38.0	36.0	38.0	30.5	38.0
38-39	35.2783752785116	38.0	36.0	38.0	30.5	38.0
40-41	35.27000817907473	38.0	36.0	38.0	30.5	38.0
42-43	35.26279273096485	38.0	36.0	38.0	30.0	38.0
44-45	35.211490189810945	37.5	35.5	38.0	27.5	38.0
46-47	35.23499327811675	38.0	36.0	38.0	28.5	38.0
48-49	35.24469065235172	38.0	36.0	38.0	29.5	38.0
50-51	35.139312205623824	37.0	35.0	38.0	27.0	38.0
52-53	35.14260263798663	37.0	35.0	38.0	27.0	38.0
54-55	35.163139636548244	37.0	35.0	38.0	27.0	38.0
56-57	35.087088343408325	37.0	35.0	38.0	27.0	38.0
58-59	35.07426505842868	37.0	35.0	38.0	27.0	38.0
60-61	35.07915370079628	37.0	35.0	38.0	27.0	38.0
62-63	35.04781468284933	37.0	35.0	38.0	27.0	38.0
64-65	34.98456317160075	37.0	35.0	38.0	27.0	38.0
66-67	34.97770497043311	37.0	35.0	38.0	27.0	38.0
68-69	34.92146208011732	37.0	35.0	38.0	27.0	38.0
70-71	34.8929387321494	37.0	35.0	38.0	27.0	38.0
72-73	34.860828812906014	37.0	35.0	38.0	27.0	38.0
74-75	34.86668108189416	37.0	35.0	38.0	27.0	38.0
76	33.8805949101712	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	5.0
21	15.0
22	24.0
23	68.0
24	143.0
25	278.0
26	510.0
27	949.0
28	1440.0
29	2298.0
30	3377.0
31	4666.0
32	6370.0
33	8312.0
34	10792.0
35	14021.0
36	19981.0
37	33119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.49546390395698	21.056886874935365	10.583910725869378	25.863738495238277
2	21.637883217854824	21.289097387396705	32.70783780988822	24.365181584860252
3	19.767977512245107	23.910161795259896	28.483862779569236	27.83799791292576
4	14.273895589880512	17.427069916987094	36.86882456354765	31.430209929584745
5	14.006900506726582	35.8111855897865	35.58461581851856	14.597298084968365
6	31.229963617219305	38.161494420366836	17.087685321851293	13.52085664056257
7	28.98682886931343	30.10275550207297	21.87667459504179	19.03374103357181
8	25.40965882917015	36.34517575609435	18.879560774285743	19.365604640449753
9	24.742171121285335	15.9294531301413	21.316360969831436	38.01201477874193
10-11	24.909043048256542	25.714258853613366	27.5963861651421	21.780311932987996
12-13	24.786356927300247	23.57500775601914	27.52728708552304	24.111348231157574
14-15	22.386221549511607	24.852635636322614	24.17621675488159	28.58492605928419
16-17	23.20553920785191	28.218747943479773	25.532344950126447	23.04336789854187
18-19	22.85064257443428	27.64292227998759	26.80198178040595	22.704453365172185
20-21	22.192556101871787	27.547969803232146	26.730062330190187	23.52941176470588
22-23	22.557324032377853	27.522586467861878	27.144186746138445	22.77590275362183
24-25	22.986490424841826	27.688988333066966	26.583403059161974	22.741118182929238
26-27	22.230631104927188	27.734114262614106	27.19213304628228	22.843121586176423
28-29	22.237682031418927	27.815434948152184	27.73740469497692	22.209478325451965
30-31	22.530530511709237	28.000169222235805	27.210465455160808	22.25883481089415
32-33	21.979618121821208	28.125675713788787	27.448786770581656	22.445919393808346
34-35	22.934313568802942	27.354304355592323	27.204354652201303	22.507027423403436
36-37	23.198958343126286	27.41306207635683	26.754035480262107	22.633944100254773
38-39	21.937782624636878	27.91508804256879	27.741635250871965	22.405494081922363
40-41	22.301140369844596	26.91855709840273	28.052346078274688	22.727956453477987
42-43	22.452500258533973	27.09059970480121	27.78770130395134	22.66919873271348
44-45	21.70416192687719	27.321870093730315	28.030253175267227	22.94371480412526
46-47	22.502326805742275	26.695747821263716	27.702150062518214	23.099775310475795
48-49	21.8945369421542	26.753565418495988	28.28079609660709	23.071101542742717
50-51	21.599338153033308	26.65767281820831	28.606078838759412	23.136910189998964
52-53	21.479002340907595	26.362944090853542	29.283907905498786	22.874145662740084
54-55	21.030563416032866	27.099060816591305	28.421814626441915	23.44856114093392
56-57	21.198845528302417	26.574471885605767	28.562833156276735	23.66384942981508
58-59	20.814335003619476	26.5899839238876	29.2171591347103	23.378521937782626
60-61	21.066758172023803	27.087779334204516	28.176442384529327	23.669020109242357
62-63	20.07257753668832	27.235378728764957	28.560482847446156	24.131560887100566
64-65	20.57695381173086	27.574293262134645	27.725183089057904	24.12356983707659
66-67	20.47213003788698	27.432334608767594	27.493912700128796	24.60162265321663
68-69	20.296796999125686	27.931540204382856	27.217986443418663	24.55367635307279
70-71	20.855230377271575	27.45959819120232	27.130554954921077	24.554616476605027
72-73	20.542169241038273	27.037012663463976	27.1780311932988	25.24278690219895
74-75	20.525717079224208	27.54985005029661	26.851808327614247	25.07262454286493
76	20.99295847474358	27.33409169964933	26.67600522708684	24.996944598520248
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	1.5
14	6.0
15	9.5
16	24.5
17	40.0
18	59.0
19	99.5
20	149.0
21	177.0
22	226.0
23	326.5
24	455.0
25	532.0
26	647.0
27	883.5
28	1171.0
29	1337.0
30	1467.5
31	1734.5
32	2126.5
33	2382.0
34	2579.0
35	3057.0
36	3690.5
37	4043.0
38	4347.0
39	5123.0
40	5783.5
41	6146.5
42	6321.0
43	6455.0
44	6498.5
45	6400.5
46	6393.0
47	6171.0
48	5627.5
49	5120.5
50	4935.0
51	4495.5
52	3633.0
53	3063.0
54	2916.0
55	2747.5
56	2305.5
57	1885.5
58	1739.0
59	1508.0
60	1191.5
61	1045.5
62	985.0
63	879.5
64	761.5
65	702.0
66	642.5
67	630.0
68	650.5
69	635.0
70	572.5
71	546.0
72	547.0
73	508.5
74	456.5
75	444.0
76	407.5
77	334.5
78	266.0
79	234.0
80	218.5
81	183.5
82	128.0
83	92.0
84	83.0
85	58.5
86	32.0
87	21.0
88	15.5
89	8.0
90	6.5
91	5.5
92	4.0
93	3.5
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	106369.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.38319435173782
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.49127341232153	44.980210399646516
2	14.68028460664186	17.728849570833606
3	4.4325771847607776	8.029595088794668
4	1.9150228090115056	4.6254077785821055
5	1.1038627411293962	3.3327379217629196
6	0.7815784147347772	2.8316520790831916
7	0.5153435364087873	2.1782662241818573
8	0.40168771115851093	1.9404149705271272
9	0.3580936959940214	1.94605571172052
>10	1.314048171386757	11.712999088080174
>50	0.004670787339052453	0.1814438417208021
>100	0.0	0.0
>500	0.0015569291130174846	0.5123673250665137
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT	545	0.5123673250665137	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	9.401235322321353E-4	0.0
11	0.0	0.0	0.0	9.401235322321353E-4	0.0
12	0.0	0.0	0.0	9.401235322321353E-4	0.0
13	0.0	0.0	0.0	9.401235322321353E-4	0.0
14	0.0	0.0	0.0	9.401235322321353E-4	0.0
15	0.0	0.0	0.0	9.401235322321353E-4	0.0
16	0.0	0.0	0.0	9.401235322321353E-4	0.0
17	0.0	0.0	0.0	9.401235322321353E-4	0.0
18	0.0	0.0	0.0	9.401235322321353E-4	0.0
19	0.0	0.0	0.0	9.401235322321353E-4	0.0
20	0.0	0.0	0.0	9.401235322321353E-4	0.0
21	0.0	0.0	0.0	0.002820370596696406	0.0
22	0.0	0.0	0.0	0.002820370596696406	0.0
23	0.0	0.0	0.0	0.003760494128928541	0.0
24	0.0	0.0	0.0	0.003760494128928541	0.0
25	0.0	0.0	0.0	0.003760494128928541	0.0
26	0.0	0.0	0.0	0.005640741193392812	0.0
27	0.0	0.0	0.0	0.008461111790089218	0.0
28	0.0	0.0	0.0	0.01222160591901776	0.0
29	0.0	0.0	0.0	0.016922223580178437	0.0
30	0.0	0.0	0.0	0.022562964773571247	0.0
31	0.0	0.0	0.0	0.03478457069258901	0.0
32	0.0	0.0	0.0	0.04418580601491036	0.0
33	0.0	0.0	0.0	0.0639284001917852	0.0
34	0.0	0.0	0.0	0.09777284735214208	0.0
35	0.0	0.0	0.0	0.13725803570589176	0.0
36	0.0	0.0	0.0	0.21152779475223044	0.0
37	0.0	0.0	0.0	0.333743853942408	0.0
38	0.0	0.0	0.0	0.45972040726151414	0.0
39	0.0	0.0	0.0	0.6543259784335662	0.0
40	0.0	0.0	0.0	0.8714945143791895	0.0
41	0.0	0.0	0.0	1.1243877445496337	0.0
42	0.0	0.0	0.0	1.4007840630258817	0.0
43	0.0	0.0	0.0	1.7147853227914147	0.0
44	0.0	0.0	0.0	2.114337823990072	0.0
45	0.0	0.0	0.0	2.543974278220158	0.0
46	0.0	0.0	0.0	3.036599009109797	0.0
47	0.0	0.0	0.0	3.514181763483722	0.0
48	0.0	0.0	0.0	4.004926247308896	0.0
49	0.0	0.0	0.0	4.459005913377018	0.0
50	0.0	0.0	0.0	4.887702244074871	0.0
51	0.0	0.0	0.0	5.3361411689496	0.0
52	0.0	0.0	0.0	5.774238734969775	0.0
53	0.0	0.0	0.0	6.22079741278004	0.0
54	0.0	0.0	0.0	6.703080784815125	0.0
55	0.0	0.0	0.0	7.267154904154406	0.0
56	0.0	0.0	0.0	7.741917287931634	0.0
57	0.0	0.0	0.0	8.265566095384933	0.0
58	0.0	0.0	0.0	8.77887354398368	0.0
59	0.0	0.0	0.0	9.291240869050194	0.0
60	0.0	0.0	0.0	9.766943376359654	0.0
61	0.0	0.0	0.0	10.207861312976526	0.0
62	0.0	0.0	0.0	10.68544406735045	0.0
63	0.0	0.0	0.0	11.144224351079732	0.0
64	0.0	0.0	0.0	11.634968834904907	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACTTCG	15	0.002219342	70.0	59
GATCTCG	70	0.0	55.0	36
CTCGTAT	90	0.0	54.444447	39
GCGAAGT	20	0.00693479	52.5	32
GGTTATC	20	0.00693479	52.5	7
CGTATGC	100	0.0	52.499996	41
CGTCTTC	100	0.0	52.499996	48
TATGCCG	100	0.0	52.499996	43
TCGTATG	100	0.0	52.499996	40
TCTCGTA	75	0.0	51.333336	38
ATGCCGT	100	0.0	48.999996	44
ATCTCGT	80	0.0	48.125	37
CCGTCTT	110	0.0	47.727276	47
GTATGCC	115	0.0	45.652176	42
TGCCGTC	110	0.0	44.545456	45
GCCGTCT	120	0.0	40.833332	46
AGATCTC	95	3.6379788E-12	40.526318	35
TACAGAT	110	0.0	38.18182	32
CTTGAAA	120	0.0	37.916664	57
TCTTCTG	135	0.0	36.296295	50
>>END_MODULE