FastQCFastQC Report
Wed 25 May 2016
SRR1781193_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781193_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116698
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGG150.002219882370.0000113
CCGTATG150.002219882370.0000112
ACCGTAT308.333178E-658.33333611
CTTGAGT252.3821107E-456.00000412
AAGCGCG200.006936466352.57
ATGTTAC200.006936466352.524
TAGCTCG200.006936466352.510
ATAGTGC305.857584E-446.6666686
CTGAGAC350.001251136940.028
GCTTGAG350.001251136940.011
ACTAAAT350.001251136940.01
TAGTGCT350.001251136940.07
CTATGGC400.002410562735.049
TACGAAT400.002410562735.038
TACTTTT552.9771109E-431.81818425
CTTGCCA552.9771109E-431.81818470
GAGTGGA450.004292762431.11110915
GGTAAAC450.004292762431.11110935
GTGGGGC450.004292762431.11110939
TCTAGTA450.004292762431.1111093
Produced by FastQC (version 0.11.5)