##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781193_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	116698
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.285429056196335	34.0	34.0	34.0	31.0	34.0
2	32.26009871634475	34.0	34.0	34.0	27.0	34.0
3	32.24733928602033	34.0	34.0	34.0	27.0	34.0
4	32.36921798145641	34.0	34.0	34.0	31.0	34.0
5	32.356715624946446	34.0	34.0	34.0	31.0	34.0
6	35.45214142487446	38.0	36.0	38.0	31.0	38.0
7	35.32108519426211	38.0	36.0	38.0	28.0	38.0
8	35.23561672008089	38.0	36.0	38.0	27.0	38.0
9	35.53736996349552	38.0	37.0	38.0	31.0	38.0
10-11	35.42872200037704	38.0	36.0	38.0	31.0	38.0
12-13	35.377962775711666	38.0	36.0	38.0	31.0	38.0
14-15	35.315836603883525	38.0	36.0	38.0	29.5	38.0
16-17	35.284503590464276	38.0	36.0	38.0	29.0	38.0
18-19	35.315382440144646	38.0	36.0	38.0	29.5	38.0
20-21	35.27912646317846	38.0	36.0	38.0	29.0	38.0
22-23	35.31809885345079	38.0	36.0	38.0	29.0	38.0
24-25	35.300356475689384	38.0	36.0	38.0	29.0	38.0
26-27	35.22904848412141	38.0	36.0	38.0	27.0	38.0
28-29	35.30951258804778	38.0	36.0	38.0	29.5	38.0
30-31	35.382320176866784	38.0	36.0	38.0	31.0	38.0
32-33	35.3424223208624	38.0	36.0	38.0	29.0	38.0
34-35	35.37032339885859	38.0	36.0	38.0	31.0	38.0
36-37	35.2528063891412	38.0	36.0	38.0	28.5	38.0
38-39	35.25841488286003	38.0	36.0	38.0	27.0	38.0
40-41	35.25276782806903	38.0	36.0	38.0	28.0	38.0
42-43	35.328990214056795	38.0	36.0	38.0	30.5	38.0
44-45	35.338651904916965	38.0	36.0	38.0	30.0	38.0
46-47	35.31333013419253	38.0	36.0	38.0	29.0	38.0
48-49	35.27002605014653	38.0	36.0	38.0	28.0	38.0
50-51	35.16858472296012	38.0	36.0	38.0	27.0	38.0
52-53	35.23768616428731	38.0	36.0	38.0	27.0	38.0
54-55	35.205290579101614	38.0	36.0	38.0	27.0	38.0
56-57	35.163289002382214	38.0	36.0	38.0	27.0	38.0
58-59	35.17660542597131	38.0	36.0	38.0	27.0	38.0
60-61	35.15531114500677	38.0	36.0	38.0	27.0	38.0
62-63	35.11624020977223	38.0	36.0	38.0	27.0	38.0
64-65	35.11522905276868	38.0	36.0	38.0	27.0	38.0
66-67	35.017943752249394	38.0	36.0	38.0	27.0	38.0
68-69	34.74143515741486	38.0	35.5	38.0	26.0	38.0
70-71	34.90408147526093	38.0	36.0	38.0	27.0	38.0
72-73	34.88103909235805	38.0	36.0	38.0	27.0	38.0
74-75	34.89685341651099	38.0	36.0	38.0	27.0	38.0
76	33.56596514079076	37.0	34.0	38.0	23.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	10.0
14	50.0
15	140.0
16	280.0
17	473.0
18	626.0
19	609.0
20	536.0
21	581.0
22	556.0
23	625.0
24	705.0
25	795.0
26	1050.0
27	1270.0
28	1587.0
29	1851.0
30	2364.0
31	3008.0
32	4043.0
33	5393.0
34	7615.0
35	11351.0
36	20312.0
37	50866.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.78225847915132	20.674733071689317	9.175821350837204	25.367187098322162
2	22.46310990762481	19.74241203790982	29.905396836278257	27.889081218187115
3	18.615571817854633	21.942106976983325	28.457214348146497	30.985106857015545
4	13.938542220089461	17.115974566830623	35.74954155169755	33.19594166138237
5	13.934257656515106	33.86004901540729	35.33136814684056	16.87432518123704
6	29.777716841762498	37.07175787074329	18.131416133952598	15.01910915354162
7	28.243843082143655	29.128177003890382	22.161476631990265	20.466503281975697
8	24.96700886047747	33.97915988277434	19.90436854102041	21.149462715727775
9	24.075819637011776	15.98313595777134	21.050060840802757	38.89098356441413
10-11	25.157671939536243	24.329037344256115	27.503042040137792	23.010248676069857
12-13	25.073694493478893	22.729181305592213	26.12726867641262	26.06985552451627
14-15	23.100652967488735	24.032545544910796	23.815318171691032	29.051483315909444
16-17	23.571526504310274	26.594714562374676	25.056984695538915	24.776774237776138
18-19	22.796448953709575	26.441327186412796	26.167543574011553	24.594680285866083
20-21	22.74803338531937	26.350065982279048	26.16540129222437	24.73649934017721
22-23	23.315309602563882	25.97173901866356	26.43575725376613	24.277194125006428
24-25	22.822584791513137	26.545442081269606	26.18553874102384	24.44643438619342
26-27	22.154192873913864	26.561723422852147	26.674407444857668	24.60967625837632
28-29	22.779310699412157	26.24937873828172	27.192411180997105	23.77889938130902
30-31	23.014104783286772	26.390769336235408	26.32907162076471	24.266054259713105
32-33	22.543659702822673	26.775523145212425	26.518020874393734	24.162796277571168
34-35	23.203482493273235	25.695384668117704	26.920341394025606	24.180791444583456
36-37	23.233902894651152	25.6469690997275	26.411763697749745	24.7073643078716
38-39	22.586505338566212	26.3809148400144	27.038166892320348	23.99441292909904
40-41	22.691477146137895	25.632401583574698	27.317948893725685	24.358172376561722
42-43	22.399698366724365	25.87705016367033	26.842362336972357	24.880889132632948
44-45	22.083069118579584	25.92118116848618	27.02659857066959	24.969151142264646
46-47	22.518809234091417	25.242077841951016	27.18555587927814	25.05355704467943
48-49	22.230029649179933	25.186806971841847	27.685564448405287	24.897598930572933
50-51	21.867127114432126	25.26564294160997	27.55659908481722	25.310630859140687
52-53	21.844418927488046	24.95586899518415	28.04803852679566	25.151673550532145
54-55	21.169171708169806	25.45801984609848	28.07246053916948	25.300347906562237
56-57	20.823835884076846	25.538569641296338	28.087028055322282	25.55056641930453
58-59	21.05048929716019	25.54371111758556	27.91393168691837	25.491867898335872
60-61	20.87610755968397	25.298634081132498	27.810245248418997	26.01501311076454
62-63	20.36881523248042	24.993144698281032	28.445217570138304	26.192822499100245
64-65	21.21201734391335	25.052271675607123	27.677852233971446	26.057858746508078
66-67	21.083051980325283	24.893742823356014	27.402354796140465	26.620850400178238
68-69	21.086051174827332	24.802481619222267	27.09129548064234	27.02017172530806
70-71	21.928396373545393	24.769490479699737	26.785377641433445	26.516735505321424
72-73	22.540232051963187	23.96356407136369	26.22024370597611	27.27596017069701
74-75	22.580078493204685	24.24763063634338	25.948173919004606	27.224116951447325
76	23.180345849971722	23.66535844658863	26.016726936194278	27.13756876724537
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	3.0
15	6.0
16	10.5
17	15.0
18	22.5
19	35.0
20	40.5
21	41.0
22	59.5
23	117.5
24	216.5
25	276.0
26	342.5
27	451.0
28	587.5
29	682.0
30	822.5
31	1056.0
32	1476.0
33	1803.0
34	2070.0
35	2624.0
36	3370.5
37	3830.0
38	4194.0
39	5100.0
40	6119.0
41	6824.0
42	7052.0
43	7241.5
44	7507.5
45	7439.0
46	7294.0
47	7268.5
48	6732.5
49	5939.5
50	5657.0
51	5339.5
52	4438.5
53	3554.5
54	3254.0
55	3022.0
56	2568.5
57	2089.5
58	1832.0
59	1632.0
60	1358.0
61	1213.0
62	1142.0
63	1135.5
64	1082.5
65	1076.0
66	1125.0
67	1134.0
68	1140.0
69	1114.0
70	1065.5
71	1049.0
72	1046.5
73	973.5
74	862.0
75	821.0
76	770.0
77	651.0
78	509.5
79	436.0
80	387.5
81	291.5
82	208.5
83	173.0
84	141.5
85	89.5
86	55.0
87	41.0
88	33.0
89	17.5
90	8.0
91	7.5
92	9.0
93	6.5
94	3.0
95	1.0
96	0.0
97	1.5
98	2.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	116698.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.508149239918424
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.97994638797462	35.8506572520523
2	13.769468290862202	13.90940718778385
3	5.227172474636083	7.920444223551389
4	2.507549794713447	5.066067970316544
5	1.6355060907332635	4.130319285677561
6	1.1825184079264361	3.5836089735899503
7	0.9042787825319806	3.197141339183191
8	0.6311289063825456	2.550172239455689
9	0.4954022598486648	2.2519666146806285
>10	2.6551525228190425	21.158031842876486
>50	0.011876081571714566	0.382183070832405
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	8.569127148708633E-4	0.0
5	0.0	0.0	0.0	8.569127148708633E-4	0.0
6	0.0	0.0	0.0	8.569127148708633E-4	0.0
7	0.0	0.0	0.0	0.0017138254297417266	0.0
8	0.0	0.0	0.0	0.0017138254297417266	0.0
9	0.0	0.0	0.0	0.0017138254297417266	0.0
10	0.0	0.0	0.0	0.0017138254297417266	0.0
11	0.0	0.0	0.0	0.0017138254297417266	0.0
12	0.0	0.0	0.0	0.0017138254297417266	0.0
13	0.0	0.0	0.0	0.0017138254297417266	0.0
14	0.0	0.0	0.0	0.0017138254297417266	0.0
15	0.0	0.0	0.0	0.0017138254297417266	0.0
16	0.0	0.0	0.0	0.0017138254297417266	0.0
17	0.0	0.0	0.0	0.0017138254297417266	0.0
18	0.0	0.0	0.0	0.0017138254297417266	0.0
19	0.0	0.0	0.0	0.0025707381446125896	0.0
20	0.0	0.0	0.0	0.003427650859483453	0.0
21	0.0	0.0	0.0	0.005141476289225179	0.0
22	0.0	0.0	0.0	0.007712214433837769	0.0
23	0.0	0.0	0.0	0.007712214433837769	0.0
24	0.0	0.0	0.0	0.008569127148708633	0.0
25	0.0	0.0	0.0	0.008569127148708633	0.0
26	0.0	0.0	0.0	0.008569127148708633	0.0
27	0.0	0.0	0.0	0.011996778008192086	0.0
28	0.0	0.0	0.0	0.02399355601638417	0.0
29	0.0	0.0	0.0	0.03427650859483453	0.0
30	0.0	0.0	0.0	0.04884402474763921	0.0
31	0.0	0.0	0.0	0.06084080275583129	0.0
32	0.0	0.0	0.0	0.08054979519786115	0.0
33	0.0	0.0	0.0	0.10111570035476186	0.0
34	0.0	0.0	0.0	0.14139059795369244	0.0
35	0.0	0.0	0.0	0.19537609899055683	0.0
36	0.0	0.0	0.0	0.3230560935063154	0.0
37	0.0	0.0	0.0	0.4970093746251007	0.0
38	0.0	0.0	0.0	0.7249481567807503	0.0
39	0.0	0.0	0.0	0.983735796671751	0.0
40	0.0	0.0	0.0	1.2690877307237485	0.0
41	0.0	0.0	0.0	1.5989991259490308	0.0
42	0.0	0.0	0.0	1.9811821967814358	0.0
43	0.0	0.0	0.0	2.494472912989083	0.0
44	0.0	0.0	0.0	3.040326312361823	0.0
45	0.0	0.0	0.0	3.7361394368369636	0.0
46	0.0	0.0	0.0	4.5390666506709625	0.0
47	0.0	0.0	0.0	5.32057104663319	0.0
48	0.0	0.0	0.0	6.052374505132907	0.0
49	0.0	0.0	0.0	6.83987729009923	0.0
50	0.0	0.0	0.0	7.5245505492810505	0.0
51	0.0	0.0	0.0	8.225505150045416	0.0
52	0.0	0.0	0.0	8.901609282078528	0.0
53	0.0	0.0	0.0	9.603420795557765	0.0
54	0.0	0.0	0.0	10.34293646849132	0.0
55	0.0	0.0	0.0	11.133866904317125	0.0
56	0.0	0.0	0.0	11.909372911275257	0.0
57	0.0	0.0	0.0	12.69516187081184	0.0
58	0.0	0.0	0.0	13.454386536187425	0.0
59	0.0	0.0	0.0	14.151056573377435	0.0
60	0.0	0.0	0.0	14.927419493050438	0.0
61	0.0	0.0	0.0	15.652367649831188	0.0
62	0.0	0.0	0.0	16.423589093214964	0.0
63	0.0	0.0	0.0	17.090267185384498	0.0
64	0.0	0.0	0.0	17.791221786148864	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGG	15	0.0022198823	70.00001	13
CCGTATG	15	0.0022198823	70.00001	12
ACCGTAT	30	8.333178E-6	58.333336	11
CTTGAGT	25	2.3821107E-4	56.000004	12
AAGCGCG	20	0.0069364663	52.5	7
ATGTTAC	20	0.0069364663	52.5	24
TAGCTCG	20	0.0069364663	52.5	10
ATAGTGC	30	5.857584E-4	46.666668	6
CTGAGAC	35	0.0012511369	40.0	28
GCTTGAG	35	0.0012511369	40.0	11
ACTAAAT	35	0.0012511369	40.0	1
TAGTGCT	35	0.0012511369	40.0	7
CTATGGC	40	0.0024105627	35.0	49
TACGAAT	40	0.0024105627	35.0	38
TACTTTT	55	2.9771109E-4	31.818184	25
CTTGCCA	55	2.9771109E-4	31.818184	70
GAGTGGA	45	0.0042927624	31.111109	15
GGTAAAC	45	0.0042927624	31.111109	35
GTGGGGC	45	0.0042927624	31.111109	39
TCTAGTA	45	0.0042927624	31.111109	3
>>END_MODULE