FastQCFastQC Report
Wed 25 May 2016
SRR1781193_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781193_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109065
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT10670.978315683308119No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGTT150.002219493570.032
TGTAGGT150.002219493570.017
ATCGCAA150.002219493570.030
CAAAGGC150.002219493570.014
GTGTTCA200.0069352652.511
GCCTTAC200.0069352652.569
CTCGTAT1200.052.539
GTGGATC200.0069352652.520
CCCAACG200.0069352652.521
CTCGCTA200.0069352652.557
TCGTATG1200.052.540
CTCGCCT200.0069352652.566
GAGTTGA200.0069352652.530
CGCAAAG200.0069352652.512
TGACTCG200.0069352652.528
TCTCGTA1050.049.99999638
CGTATGC1200.049.58333241
TATGCCG1200.049.58333243
CCGTCTT1150.048.69565647
ATCTCGT1100.047.72727637
Produced by FastQC (version 0.11.5)