##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781193_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	109065
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.75808921285472	34.0	34.0	34.0	31.0	34.0
2	32.92906065190483	34.0	34.0	34.0	31.0	34.0
3	33.024141566955485	34.0	34.0	34.0	31.0	34.0
4	33.17119149131252	34.0	34.0	34.0	32.0	34.0
5	33.11836977948929	34.0	34.0	34.0	31.0	34.0
6	36.31460138449548	38.0	37.0	38.0	34.0	38.0
7	36.18731031953422	38.0	37.0	38.0	33.0	38.0
8	35.87650483656535	38.0	36.0	38.0	31.0	38.0
9	35.95247788016321	38.0	36.0	38.0	32.0	38.0
10-11	35.82479713932059	38.0	36.0	38.0	31.0	38.0
12-13	35.75527437766469	38.0	36.0	38.0	31.0	38.0
14-15	35.78951084215835	38.0	36.0	38.0	31.0	38.0
16-17	35.70965479301334	38.0	36.0	38.0	31.0	38.0
18-19	35.720730756888095	38.0	36.0	38.0	31.0	38.0
20-21	35.63901343235685	38.0	36.0	38.0	31.0	38.0
22-23	35.719245404116805	38.0	36.0	38.0	31.0	38.0
24-25	35.69989455829093	38.0	36.0	38.0	31.0	38.0
26-27	35.41963049557603	38.0	36.0	38.0	31.0	38.0
28-29	35.395887773346175	38.0	36.0	38.0	31.0	38.0
30-31	35.42759822124421	38.0	36.0	38.0	31.0	38.0
32-33	35.366024847567964	38.0	36.0	38.0	31.0	38.0
34-35	35.36697382294962	38.0	36.0	38.0	31.0	38.0
36-37	35.307940219135375	38.0	36.0	38.0	30.5	38.0
38-39	35.22455874936964	38.0	36.0	38.0	29.0	38.0
40-41	35.26552972997754	38.0	36.0	38.0	30.0	38.0
42-43	35.28979049190849	38.0	36.0	38.0	30.5	38.0
44-45	35.22671801219457	38.0	36.0	38.0	29.0	38.0
46-47	35.263815156099575	38.0	36.0	38.0	29.5	38.0
48-49	35.255444001283635	38.0	36.0	38.0	30.0	38.0
50-51	35.127254389584195	37.5	35.0	38.0	27.0	38.0
52-53	35.16326502544354	37.5	35.0	38.0	27.0	38.0
54-55	35.18973547884289	38.0	35.0	38.0	27.5	38.0
56-57	35.14690780727089	37.0	35.0	38.0	27.0	38.0
58-59	35.11167652317425	37.0	35.0	38.0	27.0	38.0
60-61	35.11331774629808	37.0	35.0	38.0	27.0	38.0
62-63	35.08516480997571	37.0	35.0	38.0	27.0	38.0
64-65	35.00559299500298	37.0	35.0	38.0	27.0	38.0
66-67	34.99007472608078	37.0	35.0	38.0	27.0	38.0
68-69	34.887599138128635	37.0	35.0	38.0	27.0	38.0
70-71	34.823229266950904	37.0	35.0	38.0	27.0	38.0
72-73	34.78709943611608	37.0	35.0	38.0	27.0	38.0
74-75	34.782006143125656	37.0	35.0	38.0	27.0	38.0
76	33.711823224682526	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	3.0
21	10.0
22	31.0
23	79.0
24	179.0
25	298.0
26	556.0
27	1009.0
28	1603.0
29	2337.0
30	3334.0
31	4752.0
32	6394.0
33	8536.0
34	10871.0
35	13818.0
36	19634.0
37	35620.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.36102324302021	19.12712602576445	9.583276028056662	25.92857470315867
2	24.09113831201577	21.211204327694492	30.674368495851095	24.023288864438637
3	20.74451015449503	22.7946637326365	29.161509191766378	27.299316921102097
4	14.987392839132626	18.21115848347316	36.54976390226012	30.2516847751341
5	14.620639068445424	34.56562600284234	35.48892862054738	15.324806308164856
6	30.824737541832853	38.04245174895705	17.391463805987257	13.741346903222848
7	29.488836932104707	29.57043964608261	21.022326135790586	19.918397286022095
8	26.03309952780452	35.050657864576166	18.98867647733003	19.927566130289275
9	25.28125429789575	16.136249025810294	20.52079035437583	38.06170632191812
10-11	25.9304084720121	25.04103057809563	27.28235455920781	21.746206390684456
12-13	25.928116260945306	22.772658506395267	26.138999679090453	25.16022555356897
14-15	24.102599367349743	23.72805207903544	23.23155916196763	28.937789391647183
16-17	24.743960023838994	27.11227249805162	24.688946958235913	23.454820519873472
18-19	24.594507862283958	26.035850181084676	25.554485857057717	23.81515609957365
20-21	23.32003850914592	26.421400082519597	25.60812359602072	24.650437812313758
22-23	23.943978361527527	26.53692752028607	25.827717416219688	23.691376701966718
24-25	24.51657268601293	26.584605510475406	25.76032641085591	23.138495392655756
26-27	23.80552881309311	27.00728923119241	26.04776967863201	23.139412277082474
28-29	23.793150873332415	26.515839178471555	26.668958877733463	23.022051070462567
30-31	24.45605831384954	26.61898867647733	26.07252555815339	22.852427451519734
32-33	23.259982579195892	26.588731490395634	25.87264475312887	24.278641177279603
34-35	24.871406959152797	26.4470728464677	26.04180992985834	22.639710264521156
36-37	24.431302434328153	26.100032090954933	25.583826158712696	23.884839316004218
38-39	23.30170082061156	26.436528675560446	26.86654747169119	23.3952230321368
40-41	23.558428460092607	25.293632237656443	27.461147022417826	23.686792279833128
42-43	24.065923990281025	25.26795947370834	27.144363452986752	23.521753083023885
44-45	22.848759913812863	26.4929170678036	27.35249621785174	23.305826800531793
46-47	24.013661577958096	24.84252509971118	26.885343602438912	24.258469719891806
48-49	23.634071425296842	24.689405400449274	27.366707926465867	24.309815247788016
50-51	22.918901572456793	24.54316233438775	27.919130793563475	24.618805299591987
52-53	22.323843579516804	24.500527208545364	29.062485673680833	24.113143538257003
54-55	21.518819052858387	25.113006005592997	28.70856828496768	24.659606656580937
56-57	22.04373538715445	24.80722504928254	27.91500481364324	25.234034749919772
58-59	21.472516389309128	24.881492687846695	28.787420345665428	24.858570577178746
60-61	21.84935588869023	25.45637922339889	27.513867876954112	25.180397010956767
62-63	20.468527942052905	26.197680282400405	27.6481914454683	25.685600330078394
64-65	20.804566084445057	26.530050887085682	26.876174758171732	25.789208270297532
66-67	20.919635079998166	26.21143354880117	26.477788474762754	26.391142896437902
68-69	20.893045431623346	26.737725209737313	25.955622793746848	26.413606564892493
70-71	21.048457341952048	26.442488424334115	25.80937972768533	26.699674506028515
72-73	21.408334479438867	25.416036308623298	25.6094989226608	27.566130289277034
74-75	21.314353825700273	25.711731536239856	25.403199926649243	27.570714711410627
76	21.76225186815202	25.350937514326322	24.90258102966121	27.984229587860447
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.5
14	2.0
15	4.0
16	7.5
17	8.0
18	14.0
19	31.0
20	46.5
21	51.0
22	68.0
23	129.0
24	216.5
25	260.0
26	316.0
27	422.0
28	541.0
29	610.0
30	742.5
31	1000.5
32	1453.0
33	1780.0
34	1984.5
35	2455.5
36	3052.0
37	3382.0
38	3722.5
39	4637.5
40	5559.0
41	6210.5
42	6515.0
43	6841.5
44	7354.0
45	7357.0
46	7174.0
47	7024.5
48	6422.5
49	5620.5
50	5271.0
51	4901.5
52	4039.0
53	3361.5
54	3177.0
55	2860.5
56	2314.0
57	1871.5
58	1659.0
59	1475.5
60	1226.5
61	1103.5
62	1046.0
63	1013.0
64	987.0
65	989.5
66	986.5
67	988.0
68	1000.5
69	983.0
70	973.5
71	994.0
72	977.0
73	890.0
74	827.0
75	834.0
76	763.5
77	616.0
78	492.0
79	445.0
80	383.0
81	265.5
82	186.5
83	163.0
84	132.0
85	87.5
86	57.0
87	40.0
88	32.5
89	17.0
90	7.0
91	3.5
92	2.0
93	3.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	109065.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.34671984596341
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.94277220364177	34.020996653371846
2	15.0297287253809	14.833356255444002
3	5.67075436640654	8.39499381103012
4	2.5789669267930138	5.0905423371384035
5	1.7521367521367521	4.323110071975427
6	1.2337421033073206	3.6528675560445603
7	0.9104422147900408	3.144913583642782
8	0.7227796358231141	2.853344335946454
9	0.6354515050167224	2.8221702654380416
>10	2.5046451133407652	19.320588639801954
>50	0.016722408026755852	0.5648008068582955
>100	0.0	0.0
>500	0.0	0.0
>1k	0.001858045336306206	0.978315683308119
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT	1067	0.978315683308119	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	9.168844267180122E-4	0.0
5	0.0	0.0	0.0	9.168844267180122E-4	0.0
6	0.0	0.0	0.0	9.168844267180122E-4	0.0
7	0.0	0.0	0.0	0.0018337688534360245	0.0
8	0.0	0.0	0.0	0.0018337688534360245	0.0
9	0.0	0.0	0.0	0.0018337688534360245	0.0
10	0.0	0.0	0.0	0.0018337688534360245	0.0
11	0.0	0.0	0.0	0.0018337688534360245	0.0
12	0.0	0.0	0.0	0.0018337688534360245	0.0
13	0.0	0.0	0.0	0.0018337688534360245	0.0
14	0.0	0.0	0.0	0.0018337688534360245	0.0
15	0.0	0.0	0.0	0.0018337688534360245	0.0
16	0.0	0.0	0.0	0.0018337688534360245	0.0
17	0.0	0.0	0.0	0.0027506532801540367	0.0
18	0.0	0.0	0.0	0.0027506532801540367	0.0
19	0.0	0.0	0.0	0.003667537706872049	0.0
20	0.0	0.0	0.0	0.004584422133590061	0.0
21	0.0	0.0	0.0	0.00825195984046211	0.0
22	0.0	0.0	0.0	0.00825195984046211	0.0
23	0.0	0.0	0.0	0.00825195984046211	0.0
24	0.0	0.0	0.0	0.00825195984046211	0.0
25	0.0	0.0	0.0	0.00825195984046211	0.0
26	0.0	0.0	0.0	0.00825195984046211	0.0
27	0.0	0.0	0.0	0.012836381974052171	0.0
28	0.0	0.0	0.0	0.026589648374822354	0.0
29	0.0	0.0	0.0	0.03667537706872049	0.0
30	0.0	0.0	0.0	0.051345527896208686	0.0
31	0.0	0.0	0.0	0.06234814101682483	0.0
32	0.0	0.0	0.0	0.08527025168477513	0.0
33	0.0	0.0	0.0	0.10452482464585339	0.0
34	0.0	0.0	0.0	0.149452161555036	0.0
35	0.0	0.0	0.0	0.2044652271581167	0.0
36	0.0	0.0	0.0	0.33557970017879246	0.0
37	0.0	0.0	0.0	0.5042864346949067	0.0
38	0.0	0.0	0.0	0.7307568880942558	0.0
39	0.0	0.0	0.0	0.9865676431485811	0.0
40	0.0	0.0	0.0	1.2726355842846009	0.0
41	0.0	0.0	0.0	1.6036308623298032	0.0
42	0.0	0.0	0.0	1.9768028240040343	0.0
43	0.0	0.0	0.0	2.5012607160867373	0.0
44	0.0	0.0	0.0	3.0596433319580068	0.0
45	0.0	0.0	0.0	3.753724842983542	0.0
46	0.0	0.0	0.0	4.560583138495392	0.0
47	0.0	0.0	0.0	5.346353092192729	0.0
48	0.0	0.0	0.0	6.069774904873241	0.0
49	0.0	0.0	0.0	6.848209783156833	0.0
50	0.0	0.0	0.0	7.5285380277815985	0.0
51	0.0	0.0	0.0	8.240957227341493	0.0
52	0.0	0.0	0.0	8.932288085086874	0.0
53	0.0	0.0	0.0	9.635538440379591	0.0
54	0.0	0.0	0.0	10.356209599779948	0.0
55	0.0	0.0	0.0	11.141062669050566	0.0
56	0.0	0.0	0.0	11.90941181864026	0.0
57	0.0	0.0	0.0	12.715353229725393	0.0
58	0.0	0.0	0.0	13.470865997341035	0.0
59	0.0	0.0	0.0	14.205290423142163	0.0
60	0.0	0.0	0.0	14.973639572731857	0.0
61	0.0	0.0	0.0	15.694310732132214	0.0
62	0.0	0.0	0.0	16.471828725989088	0.0
63	0.0	0.0	0.0	17.120066015678724	0.0
64	0.0	0.0	0.0	17.8315683308119	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATCGTT	15	0.0022194935	70.0	32
TGTAGGT	15	0.0022194935	70.0	17
ATCGCAA	15	0.0022194935	70.0	30
CAAAGGC	15	0.0022194935	70.0	14
GTGTTCA	20	0.00693526	52.5	11
GCCTTAC	20	0.00693526	52.5	69
CTCGTAT	120	0.0	52.5	39
GTGGATC	20	0.00693526	52.5	20
CCCAACG	20	0.00693526	52.5	21
CTCGCTA	20	0.00693526	52.5	57
TCGTATG	120	0.0	52.5	40
CTCGCCT	20	0.00693526	52.5	66
GAGTTGA	20	0.00693526	52.5	30
CGCAAAG	20	0.00693526	52.5	12
TGACTCG	20	0.00693526	52.5	28
TCTCGTA	105	0.0	49.999996	38
CGTATGC	120	0.0	49.583332	41
TATGCCG	120	0.0	49.583332	43
CCGTCTT	115	0.0	48.695656	47
ATCTCGT	110	0.0	47.727276	37
>>END_MODULE