FastQCFastQC Report
Wed 25 May 2016
SRR1781192_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781192_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153497
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAG200.00694062152.58
TATGTCG200.00694062152.55
GTATAGG701.909575E-840.0000041
ACTAGCC400.002412928535.032
GGGGCCC400.002412928535.011
TATAGGA552.981468E-431.818182
AGGGTTA450.00429694831.1111135
GGTAGGG450.00429694831.11111336
GTACATG500.00719121927.9999981
GCATTAC500.00719121927.99999843
GTGTACA500.00719121927.9999981
GCCTACA657.927778E-426.9230772
CTACACT700.001221715925.0000024
GTCCCAA750.001825835923.33333451
TATACTG750.001825835923.3333345
CCCATAC800.002656731321.8753
GCTTCTA1154.0896783E-521.304351
ACTGCAT850.003776036420.5882348
GGTGTAG850.003776036420.5882341
TATTGAC850.003776036420.58823428
Produced by FastQC (version 0.11.5)