##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781192_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	153497
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25324273438569	34.0	34.0	34.0	27.0	34.0
2	32.396131520485746	34.0	34.0	34.0	31.0	34.0
3	32.42374118060939	34.0	34.0	34.0	31.0	34.0
4	32.48574890714477	34.0	34.0	34.0	31.0	34.0
5	32.56273412509691	34.0	34.0	34.0	31.0	34.0
6	35.800628025303425	38.0	37.0	38.0	31.0	38.0
7	35.71718014032847	38.0	37.0	38.0	31.0	38.0
8	35.54302038476322	38.0	37.0	38.0	31.0	38.0
9	35.80824380932526	38.0	37.0	38.0	31.0	38.0
10-11	35.70867183071982	38.0	37.0	38.0	31.0	38.0
12-13	35.64605822915106	38.0	37.0	38.0	31.0	38.0
14-15	35.60824315784673	38.0	37.0	38.0	31.0	38.0
16-17	35.59830159547092	38.0	37.0	38.0	31.0	38.0
18-19	35.6255105962983	38.0	37.0	38.0	31.0	38.0
20-21	35.57931751109142	38.0	37.0	38.0	31.0	38.0
22-23	35.612217176882936	38.0	37.0	38.0	31.0	38.0
24-25	35.62846505143423	38.0	37.0	38.0	31.0	38.0
26-27	35.57441187775657	38.0	37.0	38.0	31.0	38.0
28-29	35.667097728294365	38.0	37.0	38.0	31.0	38.0
30-31	35.725541867267765	38.0	37.0	38.0	31.0	38.0
32-33	35.683000319224476	38.0	37.0	38.0	31.0	38.0
34-35	35.71465566102269	38.0	37.0	38.0	31.0	38.0
36-37	35.63370293881965	38.0	37.0	38.0	31.0	38.0
38-39	35.624451943686196	38.0	37.0	38.0	31.0	38.0
40-41	35.60481963816882	38.0	37.0	38.0	31.0	38.0
42-43	35.68594500218245	38.0	37.0	38.0	31.0	38.0
44-45	35.67180791807006	38.0	37.0	38.0	31.0	38.0
46-47	35.652318937829406	38.0	37.0	38.0	31.0	38.0
48-49	35.62099585008176	38.0	37.0	38.0	31.0	38.0
50-51	35.54245685583432	38.0	37.0	38.0	31.0	38.0
52-53	35.60074138256774	38.0	37.0	38.0	31.0	38.0
54-55	35.54720613432184	38.0	37.0	38.0	31.0	38.0
56-57	35.51349863515247	38.0	37.0	38.0	31.0	38.0
58-59	35.51374293960143	38.0	37.0	38.0	31.0	38.0
60-61	35.51646286246637	38.0	37.0	38.0	31.0	38.0
62-63	35.47818849879802	38.0	37.0	38.0	31.0	38.0
64-65	35.49077832140042	38.0	37.0	38.0	31.0	38.0
66-67	35.39482530603204	38.0	36.0	38.0	31.0	38.0
68-69	35.13839358423943	38.0	36.0	38.0	27.0	38.0
70-71	35.333055369160306	38.0	36.0	38.0	29.5	38.0
72-73	35.344218453780854	38.0	36.0	38.0	30.0	38.0
74-75	35.35311439311518	38.0	36.0	38.0	30.5	38.0
76	34.2195091760751	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	19.0
14	43.0
15	162.0
16	275.0
17	427.0
18	536.0
19	578.0
20	579.0
21	581.0
22	631.0
23	760.0
24	855.0
25	1141.0
26	1246.0
27	1459.0
28	1778.0
29	2238.0
30	2848.0
31	3494.0
32	4592.0
33	6302.0
34	8740.0
35	13439.0
36	25578.0
37	75196.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.0940604702372	19.24011544199561	9.437969471716059	25.227854616051125
2	22.50532583698704	20.683791865639066	32.21235594180994	24.59852635556395
3	18.96519150211405	22.0955458412868	30.74913516225073	28.19012749434842
4	13.146836745995035	17.00554408229477	37.328416842022975	32.519202329687225
5	13.211333120517013	33.71531691173117	36.705603366841046	16.367746600910767
6	28.855287074014473	37.98054685107852	18.9821299439077	14.182036130999304
7	28.375147397017532	29.994723023902747	22.499462530212316	19.130667048867405
8	24.74120015374893	35.46388528766035	20.361961471559706	19.432953087031017
9	24.0167560278051	17.14300605223555	21.89293601829352	36.94730190166583
10-11	24.26659804426145	25.20733304233959	28.270910832133527	22.25515808126543
12-13	24.34021511821078	23.43140256812837	27.969276272500437	24.259106041160415
14-15	22.38187065545255	24.67572656143117	25.004397480081042	27.93800530303524
16-17	22.25841547391806	28.16243965680111	26.14057603731669	23.438568831964144
18-19	21.732346560519098	27.747773572121933	27.574480283002273	22.9453995843567
20-21	22.041147383987962	27.924649993159473	27.238317361251358	22.795885261601203
22-23	21.873717401643027	27.309002781813323	27.773832713342934	23.043447103200716
24-25	21.972742138282833	27.58979002846961	27.337993576421688	23.099474256825868
26-27	21.36588988709877	27.853638833332244	27.83376873815123	22.94670254141775
28-29	21.80922102712105	27.45232805852883	28.428568636520584	22.309882277829534
30-31	22.080887574349987	27.73669843710301	27.674156498172604	22.508257490374405
32-33	21.51605568838479	27.782953412770283	27.72855495547144	22.972435943373487
34-35	22.041473123253223	26.990104041121327	28.149084346925346	22.819338488700105
36-37	22.010202153788022	27.29206434001967	27.665361538010515	23.03237196818179
38-39	21.53006247679108	27.823996560193358	28.15853078561796	22.487410177397603
40-41	21.64700287302032	27.222030397988235	28.41000149840062	22.720965230590824
42-43	21.555795878746817	27.420731349798366	27.881978149410084	23.141494622044732
44-45	21.446673224883874	27.251998410392385	28.048105174693966	23.25322319002977
46-47	21.51475273132374	26.975120034919247	28.36504947979439	23.145077753962617
48-49	20.87597803214395	27.23571144712926	28.808380619816674	23.079929900910116
50-51	21.144712925985523	27.040919366502276	28.73183189247998	23.082535815032216
52-53	21.106275692684548	27.00378509026235	28.808380619816674	23.08155859723643
54-55	20.665550466784367	27.177078379382007	28.81554688365245	23.341824270181178
56-57	20.587373043121364	27.176101161586217	28.822061668957698	23.414464126334718
58-59	20.5375349355362	27.03342736340124	28.787859046105137	23.641178654957425
60-61	20.624833058626553	26.956227157534023	28.889163957601777	23.529775826237646
62-63	20.3098431891177	26.9868466484687	28.957243464041643	23.746066698371955
64-65	20.701707525228503	27.00378509026235	28.57971165560239	23.714795728906754
66-67	20.603008527853962	26.905737571418335	28.251366476217775	24.239887424509927
68-69	20.255770471084126	26.99629308716131	28.38687401056698	24.36106243118758
70-71	21.235594180993765	26.74221646025655	28.019114380085604	24.003074978664078
72-73	21.16881763161495	26.48944279041284	27.78653654468817	24.555203033284037
74-75	21.376313543587173	26.38976657524251	27.625295608383226	24.60862427278709
76	21.98153709844492	26.133409773480913	27.317797741975415	24.56725538609875
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	2.0
12	4.0
13	4.5
14	8.5
15	18.0
16	43.0
17	62.0
18	83.0
19	139.0
20	260.5
21	347.0
22	408.5
23	601.5
24	860.5
25	988.0
26	1122.5
27	1398.5
28	1761.5
29	1983.0
30	2300.5
31	2783.5
32	3392.5
33	3836.0
34	4095.5
35	4753.0
36	5648.0
37	6145.0
38	6606.5
39	7521.5
40	8305.0
41	8787.5
42	8940.0
43	9031.0
44	9091.0
45	8925.0
46	8790.0
47	8460.0
48	7570.0
49	6848.5
50	6687.0
51	6252.5
52	5225.0
53	4334.5
54	4037.0
55	3787.0
56	3271.5
57	2727.5
58	2449.0
59	2206.5
60	1786.5
61	1492.5
62	1376.0
63	1293.5
64	1150.5
65	1042.0
66	996.0
67	998.0
68	947.0
69	848.5
70	816.0
71	831.0
72	776.0
73	714.5
74	645.0
75	582.0
76	535.0
77	462.0
78	394.5
79	353.0
80	310.5
81	223.5
82	151.0
83	123.0
84	96.0
85	68.5
86	50.5
87	33.0
88	24.0
89	15.0
90	10.5
91	4.5
92	3.0
93	4.0
94	2.5
95	0.0
96	0.0
97	0.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	153497.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.1836452829697
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.00984688353869	48.65762848785318
2	13.780481517760478	17.41402112093396
3	3.8500799092643194	7.297862498941347
4	1.5486930968706503	3.914083011394359
5	0.8692065783368561	2.745982006162987
6	0.6062793215445688	2.2984162556922936
7	0.44439861834304273	1.9655107265940048
8	0.36294272310151054	1.8345635419584747
9	0.2691137804815178	1.5303230682032873
>10	1.251739959787596	12.04844394352984
>50	0.007217610970768675	0.29316533873626194
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	6.514785305250265E-4	0.0
6	0.0	0.0	0.0	6.514785305250265E-4	0.0
7	0.0	0.0	0.0	6.514785305250265E-4	0.0
8	0.0	0.0	0.0	6.514785305250265E-4	0.0
9	0.0	0.0	0.0	0.0019544355915750797	0.0
10	0.0	0.0	0.0	0.0019544355915750797	0.0
11	0.0	0.0	0.0	0.0019544355915750797	0.0
12	0.0	0.0	0.0	0.0019544355915750797	0.0
13	0.0	0.0	0.0	0.0019544355915750797	0.0
14	0.0	0.0	0.0	0.0019544355915750797	0.0
15	0.0	0.0	0.0	0.002605914122100106	0.0
16	0.0	0.0	0.0	0.002605914122100106	0.0
17	0.0	0.0	0.0	0.0032573926526251328	0.0
18	0.0	0.0	0.0	0.0032573926526251328	0.0
19	0.0	0.0	0.0	0.0032573926526251328	0.0
20	0.0	0.0	0.0	0.0032573926526251328	0.0
21	0.0	0.0	0.0	0.0032573926526251328	0.0
22	0.0	0.0	0.0	0.004560349713675186	0.0
23	0.0	0.0	0.0	0.005211828244200212	0.0
24	0.0	0.0	0.0	0.005863306774725239	0.0
25	0.0	0.0	0.0	0.0065147853052502655	0.0
26	0.0	0.0	0.0	0.0065147853052502655	0.0
27	0.0	0.0	0.0	0.009772177957875399	0.0
28	0.0	0.0	0.0	0.012378092079975504	0.0
29	0.0	0.0	0.0	0.02280174856837593	0.0
30	0.0	0.0	0.0	0.027362098282051116	0.0
31	0.0	0.0	0.0	0.03387688358730138	0.0
32	0.0	0.0	0.0	0.04560349713675186	0.0
33	0.0	0.0	0.0	0.06254193893040255	0.0
34	0.0	0.0	0.0	0.08860108015140361	0.0
35	0.0	0.0	0.0	0.13420457728815546	0.0
36	0.0	0.0	0.0	0.19674651621855802	0.0
37	0.0	0.0	0.0	0.3029375166941373	0.0
38	0.0	0.0	0.0	0.41564330247496695	0.0
39	0.0	0.0	0.0	0.5739525853925483	0.0
40	0.0	0.0	0.0	0.7452914389206303	0.0
41	0.0	0.0	0.0	0.9635367466465142	0.0
42	0.0	0.0	0.0	1.225431115917575	0.0
43	0.0	0.0	0.0	1.5185964546538369	0.0
44	0.0	0.0	0.0	1.891893652644677	6.514785305250265E-4
45	0.0	0.0	0.0	2.308839912180694	6.514785305250265E-4
46	0.0	0.0	0.0	2.7388157423272115	6.514785305250265E-4
47	0.0	0.0	0.0	3.215046548141006	6.514785305250265E-4
48	0.0	0.0	0.0	3.639810550043323	6.514785305250265E-4
49	0.0	0.0	0.0	4.055453852518291	6.514785305250265E-4
50	0.0	0.0	0.0	4.468491240871157	6.514785305250265E-4
51	0.0	0.0	0.0	4.9043303777924	6.514785305250265E-4
52	0.0	0.0	0.0	5.319973680267367	6.514785305250265E-4
53	0.0	0.0	0.0	5.744737682169684	6.514785305250265E-4
54	0.0	0.0	0.0	6.206635960311928	6.514785305250265E-4
55	0.0	0.0	0.0	6.688730072900448	6.514785305250265E-4
56	0.0	0.0	0.0	7.164960878714242	6.514785305250265E-4
57	0.0	0.0	0.0	7.62099585008176	6.514785305250265E-4
58	0.0	0.0	0.0	8.077682299979804	6.514785305250265E-4
59	0.0	0.0	0.0	8.514172915431573	6.514785305250265E-4
60	0.0	0.0	0.0	8.952617966474914	6.514785305250265E-4
61	0.0	0.0	0.0	9.397577802823507	6.514785305250265E-4
62	0.0	0.0	0.0	9.836674332397376	6.514785305250265E-4
63	0.0	0.0	0.0	10.28814895405122	6.514785305250265E-4
64	0.0	0.0	0.0	10.703792256526187	6.514785305250265E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCAG	20	0.006940621	52.5	8
TATGTCG	20	0.006940621	52.5	5
GTATAGG	70	1.909575E-8	40.000004	1
ACTAGCC	40	0.0024129285	35.0	32
GGGGCCC	40	0.0024129285	35.0	11
TATAGGA	55	2.981468E-4	31.81818	2
AGGGTTA	45	0.004296948	31.111113	5
GGTAGGG	45	0.004296948	31.111113	36
GTACATG	50	0.007191219	27.999998	1
GCATTAC	50	0.007191219	27.999998	43
GTGTACA	50	0.007191219	27.999998	1
GCCTACA	65	7.927778E-4	26.923077	2
CTACACT	70	0.0012217159	25.000002	4
GTCCCAA	75	0.0018258359	23.333334	51
TATACTG	75	0.0018258359	23.333334	5
CCCATAC	80	0.0026567313	21.875	3
GCTTCTA	115	4.0896783E-5	21.30435	1
ACTGCAT	85	0.0037760364	20.588234	8
GGTGTAG	85	0.0037760364	20.588234	1
TATTGAC	85	0.0037760364	20.588234	28
>>END_MODULE