FastQCFastQC Report
Wed 25 May 2016
SRR1781192_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781192_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145063
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT5680.3915540144626817No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTA150.002220976470.021
CGTTTAT150.002220976470.022
CGTCTTC1100.054.09090848
TATGCCG1250.053.20000543
ATACGAA200.006939858752.537
CCGTCTT1200.049.58333247
TCTCGTA1200.049.58333238
CTCGTAT1300.048.4615439
CGTATGC1350.046.66666841
TCGTATG1350.046.66666840
GAGCGTT305.862335E-446.6666649
ATGCCGT1450.043.44827744
TGCCGTC1450.043.44827745
ATCTCGT1400.042.537
GTATCTC1400.042.535
CGACTTA1500.042.028
GCCGTCT1500.042.046
GCGTTAT350.00125214540.011
TCAATCG350.00125214540.052
TATCTCG1500.039.66666836
Produced by FastQC (version 0.11.5)