FastQCFastQC Report
Wed 25 May 2016
SRR1781191_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781191_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80065
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCTA150.002217329870.0000119
GTTAATA150.002217329870.0000124
TTCGGTT150.002217329870.0000150
TGCACTG150.002217329870.000015
GGGTCTA200.006928550552.5000041
CCGTGTA200.006928550552.50000434
CATAGGC200.006928550552.50000423
TCGGTTG200.006928550552.50000451
TCTGGCG305.846504E-446.66666862
AGCTTTG350.001248784140.00000435
GTATTAG450.00428478731.1111131
GGACTGG500.007170989628.0000022
AGAAGTA500.007170989628.00000235
TGAAATT500.007170989628.00000223
TCCTAAA500.007170989628.0000022
CACCGCC500.007170989628.00000220
GTGTAGG800.0026457421.8750021
CAGAGAA800.0026457421.8750029
ACGAGGG1003.8897165E-421.00000270
ACAGAGA900.005234939519.4444458
Produced by FastQC (version 0.11.5)