##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781191_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	80065
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.165428089677135	34.0	34.0	34.0	27.0	34.0
2	32.4205333166802	34.0	34.0	34.0	31.0	34.0
3	32.388534315868355	34.0	34.0	34.0	31.0	34.0
4	32.51816648972709	34.0	34.0	34.0	31.0	34.0
5	32.52632236308	34.0	34.0	34.0	31.0	34.0
6	35.7049896958721	38.0	37.0	38.0	31.0	38.0
7	35.731455692250044	38.0	37.0	38.0	31.0	38.0
8	35.54067320302254	38.0	37.0	38.0	31.0	38.0
9	35.705651658027854	38.0	37.0	38.0	31.0	38.0
10-11	35.62120776868794	38.0	37.0	38.0	31.0	38.0
12-13	35.628089677137325	38.0	37.0	38.0	31.0	38.0
14-15	35.58936489102604	38.0	37.0	38.0	31.0	38.0
16-17	35.57475800911759	38.0	37.0	38.0	31.0	38.0
18-19	35.594723037532006	38.0	37.0	38.0	31.0	38.0
20-21	35.55129582214451	38.0	37.0	38.0	31.0	38.0
22-23	35.58771623056266	38.0	37.0	38.0	31.0	38.0
24-25	35.59527259101979	38.0	37.0	38.0	31.0	38.0
26-27	35.505582963841874	38.0	37.0	38.0	31.0	38.0
28-29	35.610016861300196	38.0	37.0	38.0	31.0	38.0
30-31	35.68261412602261	38.0	37.0	38.0	31.0	38.0
32-33	35.62634109785799	38.0	37.0	38.0	31.0	38.0
34-35	35.641641166552176	38.0	37.0	38.0	31.0	38.0
36-37	35.55858989570973	38.0	37.0	38.0	31.0	38.0
38-39	35.54725535502404	38.0	37.0	38.0	31.0	38.0
40-41	35.50832448635484	38.0	37.0	38.0	31.0	38.0
42-43	35.61837257228502	38.0	37.0	38.0	31.0	38.0
44-45	35.605570473989886	38.0	37.0	38.0	31.0	38.0
46-47	35.59620932991944	38.0	37.0	38.0	31.0	38.0
48-49	35.572528570536434	38.0	37.0	38.0	31.0	38.0
50-51	35.50688190844939	38.0	37.0	38.0	31.0	38.0
52-53	35.53636420408418	38.0	37.0	38.0	31.0	38.0
54-55	35.50878036595266	38.0	37.0	38.0	31.0	38.0
56-57	35.457322175732216	38.0	37.0	38.0	31.0	38.0
58-59	35.45148316992444	38.0	37.0	38.0	31.0	38.0
60-61	35.45892087678761	38.0	37.0	38.0	31.0	38.0
62-63	35.4186723287329	38.0	37.0	38.0	31.0	38.0
64-65	35.42507962280647	38.0	37.0	38.0	31.0	38.0
66-67	35.36750765003434	38.0	36.0	38.0	30.0	38.0
68-69	35.088034721788546	38.0	36.0	38.0	27.0	38.0
70-71	35.277312183850626	38.0	36.0	38.0	27.5	38.0
72-73	35.2773621432586	38.0	36.0	38.0	27.5	38.0
74-75	35.319677761818525	38.0	36.0	38.0	29.5	38.0
76	34.19517891712983	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	21.0
14	54.0
15	134.0
16	280.0
17	389.0
18	460.0
19	408.0
20	375.0
21	332.0
22	345.0
23	346.0
24	435.0
25	476.0
26	603.0
27	691.0
28	829.0
29	1045.0
30	1350.0
31	1651.0
32	2215.0
33	2991.0
34	4287.0
35	6783.0
36	12761.0
37	40802.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.156248048460625	18.423780678198966	9.417348404421407	26.002622868919
2	22.920127396490354	20.28976456628989	31.88034721788547	24.909760819334288
3	19.370511459439204	22.183226128770375	29.677137325922686	28.76912508586773
4	13.538999562855182	16.703928058452508	37.05114594392057	32.70592643477175
5	13.223006307375258	33.81502529195029	36.452882033347905	16.509086367326546
6	29.237494535689752	38.00162368075938	18.483731967776183	14.277149815774685
7	28.259539124461377	29.679635296321738	22.438019109473554	19.622806469743335
8	25.254480734403295	35.40123649534753	19.8326359832636	19.511646786985573
9	24.516330481483795	16.025729095110222	20.92799600324736	38.52994442015862
10-11	24.47573846249922	24.616249297445826	28.2239430462749	22.684069193780054
12-13	24.6468494348342	22.953225504277775	27.052394929120087	25.347530131767936
14-15	22.704677449572223	24.197839255604823	24.39955036532817	28.697932929494783
16-17	23.151814151002313	27.500780615749704	25.1907824892275	24.156622744020485
18-19	22.670954849185037	27.224754886654594	26.51033535252607	23.593954911634295
20-21	22.531068506838196	26.99431711734216	26.68519328045963	23.78942109536002
22-23	22.82957596952476	26.580903016299256	26.84256541559982	23.746955598576157
24-25	22.578529944420158	26.96434147255355	26.549678386311122	23.907450196715168
26-27	21.826640854305875	27.046150003122467	27.11297071129707	24.01423843127459
28-29	22.319990008118403	26.681446324861053	27.85861487541373	23.13994879160682
30-31	22.558546181227754	26.880659464185346	26.98494972834572	23.575844626241178
32-33	22.105164553800037	27.11297071129707	27.02054580653219	23.7613189283707
34-35	22.71092237556985	26.55904577530756	27.088615499906325	23.641416349216264
36-37	23.03503403484669	26.384187847374008	26.80696933741335	23.77380878036595
38-39	22.126397302191968	27.30219196902517	27.426465996377942	23.14494473240492
40-41	22.265034659339285	26.19496658964591	27.821145319427963	23.718853431586837
42-43	22.35371260850559	26.442265659151936	27.498907137950418	23.705114594392057
44-45	21.881596203084992	26.31924061699869	27.68063448448136	24.11852869543496
46-47	22.058327608817834	26.501592456129398	27.463935552363704	23.976144382689064
48-49	21.647411478173982	26.19496658964591	28.07094235933304	24.08667957284706
50-51	21.605570473989882	26.51907824892275	27.69936926247424	24.175982014613126
52-53	21.580590769999375	26.327983513395363	28.13339161930931	23.958034097295947
54-55	21.36139386748267	26.590894897895463	28.131518141510025	23.916193093111847
56-57	21.346406045088365	26.432898270155498	27.916692687191656	24.304002997564478
58-59	21.340161119090737	26.43726971835384	28.016611503153687	24.205957659401736
60-61	21.06663336039468	26.096921251483167	28.08905264472616	24.74739274339599
62-63	20.620745644164117	26.254293386623367	28.24829825766565	24.876662711546867
64-65	21.372010241678634	26.13813776306751	28.013489040154877	24.47636295509898
66-67	21.047274089802038	25.955161431337036	27.986635858365077	25.010928620495847
68-69	20.930493973646414	26.073815025291953	27.778679822644104	25.217011178417536
70-71	21.619933803784424	26.05320676949978	27.50452757134828	24.822331855367512
72-73	21.805408105913944	25.89645912695935	27.097982888902767	25.200149878223943
74-75	21.90033098107787	25.71660525822769	27.040529569724598	25.342534190969836
76	22.477986635858365	25.286954349590957	26.991819146943108	25.24323986760757
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	0.5
8	0.0
9	0.5
10	1.0
11	1.5
12	2.0
13	2.0
14	2.5
15	7.0
16	17.0
17	23.0
18	40.5
19	67.5
20	107.5
21	138.0
22	146.0
23	206.5
24	319.5
25	380.0
26	432.0
27	584.0
28	786.0
29	888.0
30	983.0
31	1156.5
32	1495.5
33	1756.0
34	1865.5
35	2235.0
36	2735.5
37	2976.0
38	3129.0
39	3599.5
40	4126.5
41	4545.5
42	4755.0
43	4735.0
44	4713.5
45	4696.5
46	4681.0
47	4564.0
48	4191.5
49	3745.5
50	3555.0
51	3299.5
52	2769.5
53	2332.5
54	2170.0
55	1996.0
56	1733.5
57	1458.0
58	1271.0
59	1145.5
60	943.5
61	809.5
62	752.0
63	722.5
64	671.5
65	649.5
66	642.5
67	636.0
68	674.5
69	682.5
70	641.5
71	631.0
72	599.0
73	574.5
74	537.5
75	493.0
76	448.5
77	399.5
78	327.5
79	260.0
80	232.0
81	169.0
82	131.5
83	129.0
84	99.0
85	55.5
86	35.0
87	28.0
88	22.0
89	11.0
90	4.5
91	1.5
92	0.0
93	0.0
94	1.0
95	1.0
96	0.0
97	0.0
98	0.5
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	80065.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.81352650971086
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.1326705865713	45.824017985386874
2	14.814814814814813	18.315118965840256
3	4.77056434503243	8.846562168238306
4	2.004404841284274	4.95597327171673
5	1.1739508193408903	3.628302004621245
6	0.7900426340142652	2.9301192780865546
7	0.5677800004041139	2.456753887466433
8	0.4121961568770079	2.0383438456254295
9	0.29096199308965265	1.6186848185848997
>10	1.0405932391747994	9.32242552925748
>50	0.0020205693964559213	0.06369824517579467
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.002497970399050771	0.0
2	0.0	0.0	0.0	0.002497970399050771	0.0
3	0.0	0.0	0.0	0.002497970399050771	0.0
4	0.0	0.0	0.0	0.0037469555985761567	0.0
5	0.0	0.0	0.0	0.0037469555985761567	0.0
6	0.0	0.0	0.0	0.0037469555985761567	0.0
7	0.0	0.0	0.0	0.0037469555985761567	0.0
8	0.0	0.0	0.0	0.0037469555985761567	0.0
9	0.0	0.0	0.0	0.0037469555985761567	0.0
10	0.0	0.0	0.0	0.0037469555985761567	0.0
11	0.0	0.0	0.0	0.0037469555985761567	0.0
12	0.0	0.0	0.0	0.0037469555985761567	0.0
13	0.0	0.0	0.0	0.0037469555985761567	0.0
14	0.0	0.0	0.0	0.0037469555985761567	0.0
15	0.0	0.0	0.0	0.0037469555985761567	0.0
16	0.0	0.0	0.0	0.0037469555985761567	0.0
17	0.0	0.0	0.0	0.0037469555985761567	0.0
18	0.0	0.0	0.0	0.004995940798101542	0.0
19	0.0	0.0	0.0	0.004995940798101542	0.0
20	0.0	0.0	0.0	0.004995940798101542	0.0
21	0.0	0.0	0.0	0.006244925997626928	0.0
22	0.0	0.0	0.0	0.007493911197152313	0.0
23	0.0	0.0	0.0	0.007493911197152313	0.0
24	0.0	0.0	0.0	0.011240866795728471	0.0
25	0.0	0.0	0.0	0.012489851995253856	0.0
26	0.0	0.0	0.0	0.012489851995253856	0.0
27	0.0	0.0	0.0	0.0174857927933554	0.0
28	0.0	0.0	0.0	0.022481733591456943	0.0
29	0.0	0.0	0.0	0.026228689190033096	0.0
30	0.0	0.0	0.0	0.03746955598576157	0.0
31	0.0	0.0	0.0	0.04621245238243927	0.0
32	0.0	0.0	0.0	0.06369824517579467	0.0
33	0.0	0.0	0.0	0.08243302316867546	0.0
34	0.0	0.0	0.0	0.10116780116155624	0.0
35	0.0	0.0	0.0	0.14862923874352088	0.0
36	0.0	0.0	0.0	0.2185724099169425	0.0
37	0.0	0.0	0.0	0.3297320926747018	0.0
38	0.0	0.0	0.0	0.4758633610191719	0.0
39	0.0	0.0	0.0	0.6432273777555736	0.0
40	0.0	0.0	0.0	0.8180853056891276	0.0
41	0.0	0.0	0.0	1.0141759820146132	0.0
42	0.0	0.0	0.0	1.2252544807344032	0.0
43	0.0	0.0	0.0	1.486292387435209	0.0
44	0.0	0.0	0.0	1.8360082433023168	0.0
45	0.0	0.0	0.0	2.199462936364204	0.0
46	0.0	0.0	0.0	2.5953912446137513	0.0
47	0.0	0.0	0.0	3.0325360644476365	0.0
48	0.0	0.0	0.0	3.468431899081996	0.0
49	0.0	0.0	0.0	3.85436832573534	0.0
50	0.0	0.0	0.0	4.259039530381565	0.0
51	0.0	0.0	0.0	4.662461749828265	0.0
52	0.0	0.0	0.0	5.0896146880659465	0.0
53	0.0	0.0	0.0	5.469306188721664	0.0
54	0.0	0.0	0.0	5.858989570973584	0.0
55	0.0	0.0	0.0	6.298632361206519	0.0
56	0.0	0.0	0.0	6.771997751826641	0.0
57	0.0	0.0	0.0	7.256604009242491	0.0
58	0.0	0.0	0.0	7.716230562667833	0.0
59	0.0	0.0	0.0	8.139636545306939	0.0
60	0.0	0.0	0.0	8.594267157934178	0.0
61	0.0	0.0	0.0	9.00518328857803	0.0
62	0.0	0.0	0.0	9.458564916005745	0.0
63	0.0	0.0	0.0	9.889464809842003	0.0
64	0.0	0.0	0.0	10.330356585274464	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCCTA	15	0.0022173298	70.00001	19
GTTAATA	15	0.0022173298	70.00001	24
TTCGGTT	15	0.0022173298	70.00001	50
TGCACTG	15	0.0022173298	70.00001	5
GGGTCTA	20	0.0069285505	52.500004	1
CCGTGTA	20	0.0069285505	52.500004	34
CATAGGC	20	0.0069285505	52.500004	23
TCGGTTG	20	0.0069285505	52.500004	51
TCTGGCG	30	5.846504E-4	46.666668	62
AGCTTTG	35	0.0012487841	40.000004	35
GTATTAG	45	0.004284787	31.111113	1
GGACTGG	50	0.0071709896	28.000002	2
AGAAGTA	50	0.0071709896	28.000002	35
TGAAATT	50	0.0071709896	28.000002	23
TCCTAAA	50	0.0071709896	28.000002	2
CACCGCC	50	0.0071709896	28.000002	20
GTGTAGG	80	0.00264574	21.875002	1
CAGAGAA	80	0.00264574	21.875002	9
ACGAGGG	100	3.8897165E-4	21.000002	70
ACAGAGA	90	0.0052349395	19.444445	8
>>END_MODULE