FastQCFastQC Report
Wed 25 May 2016
SRR1781191_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781191_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76094
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT7190.9448839593134808No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG150.002216902370.01
TATCAGG252.3768176E-456.0000042
GCTATAC200.006927225752.53
TACATGA200.006927225752.549
CAGTGTA200.006927225752.556
GTTGGCG200.006927225752.557
TATGCCG1450.050.68965543
AATCTCG1450.050.68965536
CTCGTAT1500.049.039
TCTCGTA1500.049.038
TGCCGTC1500.049.045
CGTATGC1450.048.2758641
GTATGCC1450.048.2758642
TCGTATG1450.048.2758640
CGTCTTC1500.046.66666848
CCGTCTT1500.046.66666847
ATCTCGT1600.045.937537
GTCTTCT1550.045.16129349
GCCGTCT1550.045.16129346
ATGCCGT1650.044.54545644
Produced by FastQC (version 0.11.5)