FastQCFastQC Report
Wed 25 May 2016
SRR1781190_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781190_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40563
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAAGT200.006904010752.50000428
GGGCCCA200.006904010752.5000045
GCTAAAG200.006904010752.50000427
AGCAGCA200.006904010752.5000047
CTCAGGA200.006904010752.50000417
CCCACTG350.001241499440.09
TTCGCAG350.001241499440.036
CACTGCA500.00712991327.99999815
ATCCTAT700.001206261325.06
CTCAGAA700.001206261325.020
CGACGAG800.002623458921.87500270
CTGACGC2751.8946196E-411.45454570
TCTGACG3054.94262E-410.32786869
ATCTGAC3600.00225478468.74999968
CATCTGA3700.00289091898.51351467
ACATCTG3750.00326438648.40000166
CACATCT3900.0046510348.07692365
ACACATC4100.00728846437.68292764
TACACAT4100.00728846437.68292763
Produced by FastQC (version 0.11.5)