##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781190_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	40563
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37216182235042	34.0	34.0	34.0	31.0	34.0
2	32.55708404210734	34.0	34.0	34.0	31.0	34.0
3	32.55922885388161	34.0	34.0	34.0	31.0	34.0
4	32.605305327515225	34.0	34.0	34.0	31.0	34.0
5	32.60471365530163	34.0	34.0	34.0	31.0	34.0
6	35.89495352907822	38.0	37.0	38.0	32.0	38.0
7	35.705174666568055	38.0	37.0	38.0	31.0	38.0
8	35.65276236964721	38.0	37.0	38.0	31.0	38.0
9	35.88307077878855	38.0	37.0	38.0	32.0	38.0
10-11	35.80426743584054	38.0	37.0	38.0	31.5	38.0
12-13	35.7942952937406	38.0	37.0	38.0	31.0	38.0
14-15	35.76530335527451	38.0	37.0	38.0	31.0	38.0
16-17	35.758757981411634	38.0	37.0	38.0	31.5	38.0
18-19	35.787330818726424	38.0	37.0	38.0	31.5	38.0
20-21	35.773673051795974	38.0	37.0	38.0	31.0	38.0
22-23	35.78152503513054	38.0	37.0	38.0	31.5	38.0
24-25	35.79992850627419	38.0	37.0	38.0	31.5	38.0
26-27	35.748477676700446	38.0	37.0	38.0	31.5	38.0
28-29	35.85894780958016	38.0	37.0	38.0	32.0	38.0
30-31	35.89468234598033	38.0	37.0	38.0	32.5	38.0
32-33	35.837955772502035	38.0	37.0	38.0	32.0	38.0
34-35	35.855089613687355	38.0	37.0	38.0	32.0	38.0
36-37	35.785937923723594	38.0	37.0	38.0	31.5	38.0
38-39	35.76906293913172	38.0	37.0	38.0	31.0	38.0
40-41	35.79223676749747	38.0	37.0	38.0	31.5	38.0
42-43	35.858725932500064	38.0	37.0	38.0	32.0	38.0
44-45	35.86068584670759	38.0	37.0	38.0	32.0	38.0
46-47	35.81643369573256	38.0	37.0	38.0	32.0	38.0
48-49	35.81009787244533	38.0	37.0	38.0	32.0	38.0
50-51	35.74216650642211	38.0	37.0	38.0	31.0	38.0
52-53	35.77077632325025	38.0	37.0	38.0	31.5	38.0
54-55	35.73936838991199	38.0	37.0	38.0	31.0	38.0
56-57	35.69890047580307	38.0	37.0	38.0	31.0	38.0
58-59	35.70311614032492	38.0	37.0	38.0	31.0	38.0
60-61	35.69429036313882	38.0	37.0	38.0	31.0	38.0
62-63	35.661588146833324	38.0	37.0	38.0	31.0	38.0
64-65	35.68564948351947	38.0	37.0	38.0	31.0	38.0
66-67	35.591018908857826	38.0	37.0	38.0	31.0	38.0
68-69	35.27679165742178	38.0	36.5	38.0	29.0	38.0
70-71	35.49944530729975	38.0	37.0	38.0	31.0	38.0
72-73	35.52185489238961	38.0	37.0	38.0	31.0	38.0
74-75	35.54544782190666	38.0	37.0	38.0	31.0	38.0
76	34.4205063728028	37.0	34.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	15.0
14	37.0
15	66.0
16	112.0
17	178.0
18	177.0
19	170.0
20	156.0
21	149.0
22	179.0
23	148.0
24	214.0
25	212.0
26	295.0
27	326.0
28	418.0
29	464.0
30	609.0
31	773.0
32	972.0
33	1353.0
34	1944.0
35	3192.0
36	6394.0
37	22009.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.94800680423045	19.57695436728053	10.945935951482879	26.52910287700614
2	22.40465448808027	20.148904173754406	32.35707418090378	25.089367157261545
3	18.709168454009813	21.941177920765227	28.851416315361288	30.49823730986367
4	13.344673717427211	16.125533121317456	36.43221655203018	34.09757660922516
5	12.965017380371274	33.89788723713729	36.77982397751646	16.357271404974977
6	29.608263688583193	37.26548825284126	18.100239134186328	15.026008924389222
7	28.846485713581345	28.82429800557158	22.633927470847816	19.695288809999262
8	25.096763059931465	34.679387619258925	19.853068066957572	20.370781253852034
9	23.984912358553363	15.812439908290807	21.48509725612011	38.71755047703572
10-11	24.274585213125263	25.099228360821442	27.84680620269704	22.77938022335626
12-13	24.389221704509033	23.059191874368267	27.309370608682787	25.242215812439913
14-15	22.27769149224663	24.28691171757513	24.819416709809435	28.615980080368807
16-17	22.488474718339376	27.401819392056805	25.93743066341247	24.172275226191356
18-19	22.028696102359294	27.3549786751473	26.98518354165126	23.631141680842145
20-21	21.729162044227497	27.234178931538594	27.080097625915243	23.956561398318666
22-23	22.271528240021695	26.859453196262606	26.943273426521706	23.925745137193992
24-25	21.726696743337524	27.105983285259967	27.032024258560757	24.135295712841753
26-27	21.583709291719057	27.17008110839928	27.442496856741368	23.803712743140302
28-29	22.170450903532775	27.133101595049673	28.00828341099031	22.688164090427236
30-31	22.08663067327367	27.036954860340707	27.290880852007987	23.585533614377635
32-33	21.509750265019846	27.295811453787934	27.10968123659493	24.084757044597293
34-35	22.44040135098489	26.874245001602446	27.175011710179227	23.510341937233438
36-37	22.410817740305205	26.304760496018538	27.075167024135293	24.20925473954096
38-39	21.773537460247024	27.210758573083844	27.97130389764071	23.044400069028427
40-41	21.578778689939107	26.37871952271775	28.154968813943743	23.887532973399402
42-43	22.007741044794518	26.92601632029189	27.401819392056805	23.66442324285679
44-45	21.384019919631193	26.616621058600202	27.60150876414467	24.397850257623944
46-47	21.45058304366048	26.83356753691788	27.9811651012006	23.73468431822104
48-49	21.186795848433302	26.625249611715105	28.36821734092646	23.81973719892513
50-51	21.074624657939502	26.95066932919163	27.87022656115179	24.104479451717083
52-53	21.388950521411136	26.234499420654288	28.620910682148754	23.755639375785815
54-55	20.89465769297143	27.00860390010601	28.081009787244533	24.015728619678033
56-57	20.52609520992037	26.879175603382393	28.21413603530311	24.380593151394127
58-59	20.7381110864581	26.74111875354387	28.390405048936223	24.130365111061806
60-61	20.381875107856914	26.342972659813128	28.400266252496113	24.874885979833838
62-63	20.088504301950053	26.301062544683578	28.71459211596775	24.895841037398615
64-65	21.051204299484752	26.591968049700466	28.07114858368464	24.285679067130143
66-67	20.799743608707445	26.262850380888985	27.80243078667751	25.13497522372606
68-69	20.443507630106257	26.473633606981732	27.619998520819465	25.46286024209255
70-71	21.381554618741216	25.822794172028697	27.748194167098095	25.04745704213199
72-73	21.493725809235016	25.780884056899144	27.316766511352707	25.408623622513126
74-75	21.649039765303353	25.676108769075267	27.130636294159704	25.544215171461676
76	22.495870621009296	25.382737963168406	26.763306461553633	25.358084954268666
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	2.0
15	2.5
16	8.0
17	14.0
18	16.0
19	32.5
20	54.5
21	62.0
22	73.5
23	104.0
24	152.5
25	182.0
26	212.0
27	286.5
28	371.0
29	411.0
30	488.0
31	622.0
32	789.0
33	899.0
34	966.5
35	1139.5
36	1349.0
37	1453.0
38	1534.0
39	1817.0
40	2166.0
41	2408.0
42	2503.0
43	2446.5
44	2428.5
45	2433.5
46	2400.0
47	2312.0
48	2107.5
49	1936.0
50	1881.0
51	1790.5
52	1518.0
53	1259.0
54	1182.0
55	1064.5
56	867.5
57	720.5
58	653.0
59	570.5
60	463.5
61	413.0
62	387.0
63	374.0
64	346.0
65	312.0
66	281.0
67	269.0
68	284.5
69	272.5
70	275.0
71	305.0
72	289.0
73	250.5
74	204.5
75	181.0
76	174.0
77	155.0
78	120.5
79	98.0
80	86.5
81	68.5
82	51.5
83	41.0
84	36.0
85	21.5
86	11.0
87	10.0
88	9.0
89	5.0
90	3.0
91	3.0
92	2.0
93	2.5
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	40563.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.86480289919385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.6310665504214	52.00552227399354
2	13.92763731473409	18.90392722431773
3	4.5117698343504795	9.185711116041713
4	1.9289450741063645	5.236299090303972
5	1.0498401627433884	3.562359786011883
6	0.6647776809067132	2.7069003771910363
7	0.3814298169136879	1.8119961541306115
8	0.2688172043010753	1.4594581268643838
9	0.1961639058413252	1.19813623252718
>10	0.43955245568148793	3.929689618617952
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0024653008899736214	0.0
6	0.0	0.0	0.0	0.0024653008899736214	0.0
7	0.0	0.0	0.0	0.0024653008899736214	0.0
8	0.0	0.0	0.0	0.0024653008899736214	0.0
9	0.0	0.0	0.0	0.0024653008899736214	0.0
10	0.0	0.0	0.0	0.0024653008899736214	0.0
11	0.0	0.0	0.0	0.004930601779947243	0.0
12	0.0	0.0	0.0	0.007395902669920864	0.0
13	0.0	0.0	0.0	0.007395902669920864	0.0
14	0.0	0.0	0.0	0.007395902669920864	0.0
15	0.0	0.0	0.0	0.007395902669920864	0.0
16	0.0	0.0	0.0	0.007395902669920864	0.0
17	0.0	0.0	0.0	0.007395902669920864	0.0
18	0.0	0.0	0.0	0.007395902669920864	0.0
19	0.0	0.0	0.0	0.009861203559894486	0.0
20	0.0	0.0	0.0	0.009861203559894486	0.0
21	0.0	0.0	0.0	0.009861203559894486	0.0
22	0.0	0.0	0.0	0.009861203559894486	0.0
23	0.0	0.0	0.0	0.009861203559894486	0.0
24	0.0	0.0	0.0	0.009861203559894486	0.0
25	0.0	0.0	0.0	0.009861203559894486	0.0
26	0.0	0.0	0.0	0.009861203559894486	0.0
27	0.0	0.0	0.0	0.009861203559894486	0.0
28	0.0	0.0	0.0	0.014791805339841728	0.0
29	0.0	0.0	0.0	0.024653008899736213	0.0
30	0.0	0.0	0.0	0.027118309789709832	0.0
31	0.0	0.0	0.0	0.03204891156965708	0.0
32	0.0	0.0	0.0	0.03944481423957794	0.0
33	0.0	0.0	0.0	0.051771318689446046	0.0
34	0.0	0.0	0.0	0.09121613292902399	0.0
35	0.0	0.0	0.0	0.1281956462786283	0.0
36	0.0	0.0	0.0	0.1725710622981535	0.0
37	0.0	0.0	0.0	0.2761136996770456	0.0
38	0.0	0.0	0.0	0.40184404506570026	0.0
39	0.0	0.0	0.0	0.559623302024012	0.0
40	0.0	0.0	0.0	0.7667085767817963	0.0
41	0.0	0.0	0.0	0.9910509577693958	0.0
42	0.0	0.0	0.0	1.2868870645662303	0.0
43	0.0	0.0	0.0	1.634494490052511	0.0
44	0.0	0.0	0.0	2.095505756477578	0.0
45	0.0	0.0	0.0	2.5688435273525134	0.0
46	0.0	0.0	0.0	3.0890220151369476	0.0
47	0.0	0.0	0.0	3.572220989571777	0.0
48	0.0	0.0	0.0	4.119517787145921	0.0
49	0.0	0.0	0.0	4.5509454428913045	0.0
50	0.0	0.0	0.0	4.979907797746715	0.0
51	0.0	0.0	0.0	5.401474249932204	0.0
52	0.0	0.0	0.0	5.862485516357271	0.0
53	0.0	0.0	0.0	6.3456844907921015	0.0
54	0.0	0.0	0.0	6.838744668786825	0.0
55	0.0	0.0	0.0	7.371249661021127	0.0
56	0.0	0.0	0.0	7.884032246135641	0.0
57	0.0	0.0	0.0	8.411606636589996	0.0
58	0.0	0.0	0.0	8.870152602125088	0.0
59	0.0	0.0	0.0	9.32623326677021	0.0
60	0.0	0.0	0.0	9.819293444764934	0.0
61	0.0	0.0	0.0	10.297561817419815	0.0
62	0.0	0.0	0.0	10.7289894731652	0.0
63	0.0	0.0	0.0	11.249167960949634	0.0
64	0.0	0.0	0.0	11.853166678993171	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAAAGT	20	0.0069040107	52.500004	28
GGGCCCA	20	0.0069040107	52.500004	5
GCTAAAG	20	0.0069040107	52.500004	27
AGCAGCA	20	0.0069040107	52.500004	7
CTCAGGA	20	0.0069040107	52.500004	17
CCCACTG	35	0.0012414994	40.0	9
TTCGCAG	35	0.0012414994	40.0	36
CACTGCA	50	0.007129913	27.999998	15
ATCCTAT	70	0.0012062613	25.0	6
CTCAGAA	70	0.0012062613	25.0	20
CGACGAG	80	0.0026234589	21.875002	70
CTGACGC	275	1.8946196E-4	11.454545	70
TCTGACG	305	4.94262E-4	10.327868	69
ATCTGAC	360	0.0022547846	8.749999	68
CATCTGA	370	0.0028909189	8.513514	67
ACATCTG	375	0.0032643864	8.400001	66
CACATCT	390	0.004651034	8.076923	65
ACACATC	410	0.0072884643	7.682927	64
TACACAT	410	0.0072884643	7.682927	63
>>END_MODULE