FastQCFastQC Report
Wed 25 May 2016
SRR1781190_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781190_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38688
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT3200.8271298593879239No Hit
ATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCTT410.10597601323407775Illumina PCR Primer Index 10 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATC150.00220862870.0000113
TAGTGGT150.00220862870.0000129
GGCAGGC207.832232E-570.035
GCGCCAA207.832232E-570.040
CACAAAT207.832232E-570.037
CTGTGGT207.832232E-570.014
TCCTGTG252.3621444E-455.99999612
CATTCGT252.3621444E-455.99999619
ATAGTCC252.3621444E-455.9999968
CGCCAAG252.3621444E-455.99999641
AACAAGA200.006901569752.534
GTCGAAA200.006901569752.511
TACCAAT200.006901569752.541
GCTGGTA200.006901569752.552
GAGTCGA200.006901569752.59
TTCGTTT200.006901569752.521
ATTCGTT200.006901569752.520
ATGCTGG200.006901569752.515
CAATTCG200.006901569752.544
TGAGTCG200.006901569752.58
Produced by FastQC (version 0.11.5)