##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781190_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	38688
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29295905707196	34.0	34.0	34.0	27.0	34.0
2	32.68543217535153	34.0	34.0	34.0	31.0	34.0
3	32.85246071133168	34.0	34.0	34.0	31.0	34.0
4	33.06371484698098	34.0	34.0	34.0	31.0	34.0
5	32.94535773366419	34.0	34.0	34.0	31.0	34.0
6	35.92204301075269	38.0	36.0	38.0	32.0	38.0
7	35.873914392059554	38.0	36.0	38.0	32.0	38.0
8	35.415942928039705	37.0	35.0	38.0	31.0	38.0
9	35.59442204301075	37.0	36.0	38.0	31.0	38.0
10-11	35.49776416459884	37.0	36.0	38.0	31.0	38.0
12-13	35.47052057485525	37.0	36.0	38.0	31.0	38.0
14-15	35.491340984284534	37.5	36.0	38.0	31.0	38.0
16-17	35.41355200578991	37.0	35.5	38.0	31.0	38.0
18-19	35.419677936311004	37.0	35.5	38.0	31.0	38.0
20-21	35.34879032258065	37.0	35.5	38.0	30.0	38.0
22-23	35.40528070719603	37.0	36.0	38.0	31.0	38.0
24-25	35.43014629859388	37.0	36.0	38.0	31.0	38.0
26-27	35.09742038875103	37.0	35.0	38.0	28.0	38.0
28-29	35.04829662944583	37.0	35.0	38.0	27.0	38.0
30-31	35.06901364764268	37.0	35.0	38.0	27.0	38.0
32-33	34.96000051695616	37.0	35.0	38.0	27.0	38.0
34-35	35.000155086848636	37.0	35.0	38.0	27.0	38.0
36-37	34.96753515301903	37.0	35.0	38.0	27.0	38.0
38-39	34.93452750206782	37.0	35.0	38.0	27.0	38.0
40-41	34.963270264681555	37.0	35.0	38.0	27.0	38.0
42-43	34.8923826509512	37.0	35.0	38.0	27.0	38.0
44-45	34.83942049214227	37.0	34.0	38.0	27.0	38.0
46-47	34.88155241935484	37.0	35.0	38.0	27.0	38.0
48-49	34.84747208436724	37.0	35.0	38.0	27.0	38.0
50-51	34.708126550868485	37.0	34.0	38.0	27.0	38.0
52-53	34.789715157154674	37.0	34.0	38.0	27.0	38.0
54-55	34.80168269230769	37.0	34.0	38.0	27.0	38.0
56-57	34.711409222497934	37.0	34.0	38.0	27.0	38.0
58-59	34.70600703060381	37.0	34.0	38.0	27.0	38.0
60-61	34.74413254755997	37.0	34.0	38.0	27.0	38.0
62-63	34.64987851530191	37.0	34.0	38.0	27.0	38.0
64-65	34.57075837468983	37.0	34.0	38.0	27.0	38.0
66-67	34.54191222084367	37.0	34.0	38.0	26.5	38.0
68-69	34.409093258891644	37.0	34.0	38.0	25.5	38.0
70-71	34.32081007030604	37.0	34.0	38.0	25.0	38.0
72-73	34.33934294871795	37.0	34.0	38.0	25.0	38.0
74-75	34.323213916459885	37.0	34.0	38.0	25.0	38.0
76	33.26294975186104	37.0	33.0	38.0	23.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	10.0
22	16.0
23	37.0
24	91.0
25	141.0
26	252.0
27	447.0
28	623.0
29	998.0
30	1423.0
31	2006.0
32	2651.0
33	3350.0
34	4292.0
35	5312.0
36	7274.0
37	9762.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.13709677419355	21.360628618693134	10.794044665012407	25.708229942100907
2	22.076612903225808	21.267576509511994	32.21153846153847	24.44427212572374
3	19.89505789909016	24.607113316790734	28.538564929693962	26.959263854425146
4	14.697063688999174	17.977150537634408	36.78143093465674	30.544354838709676
5	14.619520264681555	35.7345947063689	35.26157981803143	14.384305210918114
6	31.244830438378823	38.278019023986765	17.27667493796526	13.200475599669147
7	29.51044251447477	29.218362282878413	21.890508684863523	19.380686517783293
8	25.66170388751034	35.73717948717949	19.065343258891644	19.53577336641853
9	25.02843258891646	15.83436724565757	21.40198511166253	37.73521505376344
10-11	25.403225806451612	25.842638544251447	27.446495037220842	21.307640612076096
12-13	25.361869313482217	23.171267576509514	27.375413564929694	24.09144954507858
14-15	23.303091397849464	24.87205334987593	23.941532258064516	27.88332299421009
16-17	23.887251861042184	28.075889164598845	25.264940033085193	22.771918941273782
18-19	23.642990074441688	27.008374689826304	26.499172870140615	22.849462365591396
20-21	22.685328784119108	27.436155913978492	25.969292803970223	23.909222497932177
22-23	23.414236972704714	27.22678866832093	26.676230355665844	22.68274400330852
24-25	23.480148883374692	27.64423076923077	26.510804383788255	22.364815963606286
26-27	22.774503722084365	27.97895988420182	27.038099669148057	22.208436724565754
28-29	22.863678660049626	26.51209677419355	28.440343258891648	22.183881306865178
30-31	23.40906741108354	26.720171629445822	27.732113316790734	22.138647642679903
32-33	23.33410876757651	27.051023573200993	26.717586848635232	22.89728081058726
34-35	23.371588089330025	26.54828370554177	27.509822167080234	22.570306038047974
36-37	22.989040529363113	27.247466914805624	27.283653846153843	22.47983870967742
38-39	22.182588916459885	27.402553763440864	27.81224152191894	22.602615798180313
40-41	22.60390818858561	26.412582712985937	28.13275434243176	22.85075475599669
42-43	23.314722911497103	26.364764267990076	27.878153432588913	22.442359387923904
44-45	22.525072373862695	27.172508271298597	27.750206782464847	22.55221257237386
46-47	23.105355665839536	26.09206989247312	27.617090570719604	23.18548387096774
48-49	22.78355045492142	25.90596567411084	27.990591397849464	23.31989247311828
50-51	22.31053556658395	26.344086021505376	28.00093052109181	23.34444789081886
52-53	21.772901157981803	26.07009925558313	29.325630686517783	22.831368899917287
54-55	21.46272746071133	26.301437138130684	28.886217948717945	23.349617452440032
56-57	21.87887717121588	26.39190446650124	28.199958643507028	23.529259718775847
58-59	21.421370967741936	26.48366418527709	28.886217948717945	23.208746898263026
60-61	21.90343258891646	26.66589123242349	28.199958643507028	23.23071753515302
62-63	20.74674317617866	27.31984077750207	27.945357733664185	23.988058312655085
64-65	21.121536393713814	27.66878618693135	27.129859387923904	24.079818031430936
66-67	21.104735318444995	27.383167907361454	27.160876757650954	24.351220016542598
68-69	20.737696443341605	27.633891645988417	27.211279983457402	24.417131927212573
70-71	21.271453680727877	27.194478908188586	26.8261476426799	24.707919768403638
72-73	21.32961124896609	26.536652191894127	26.689154259718777	25.444582299421008
74-75	21.41232423490488	26.850703060380482	26.66201406120761	25.074958643507028
76	21.76126964433416	25.927936311000828	27.11435070306038	25.19644334160463
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	1.0
12	1.0
13	0.5
14	1.0
15	5.5
16	13.0
17	17.0
18	20.0
19	32.0
20	50.0
21	59.0
22	66.5
23	94.5
24	141.5
25	168.0
26	204.0
27	266.5
28	355.5
29	418.0
30	453.5
31	561.5
32	772.5
33	911.0
34	976.0
35	1087.0
36	1250.5
37	1368.0
38	1525.5
39	1791.0
40	2009.0
41	2237.5
42	2356.0
43	2408.0
44	2468.5
45	2412.5
46	2348.0
47	2258.5
48	2041.0
49	1862.0
50	1811.0
51	1655.0
52	1375.0
53	1166.0
54	1081.0
55	1000.0
56	826.0
57	650.0
58	567.0
59	503.5
60	406.5
61	349.5
62	326.0
63	295.5
64	277.0
65	290.0
66	287.5
67	284.0
68	281.0
69	276.5
70	258.5
71	242.0
72	252.0
73	238.0
74	207.5
75	201.0
76	179.5
77	147.5
78	125.0
79	113.0
80	87.0
81	62.0
82	52.0
83	41.0
84	29.5
85	16.5
86	11.5
87	8.0
88	7.5
89	5.0
90	1.5
91	0.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	38688.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.2178453267163
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.72134881810373	53.79704301075269
2	13.603943388476045	18.832712985938795
3	4.298144068113074	8.925248138957818
4	1.7513723440008961	4.849048800661704
5	0.9821128496209716	3.3989867659222495
6	0.5713432166996527	2.3728287841191067
7	0.32488143694686134	1.5741315136476426
8	0.23152470219201612	1.282051282051282
9	0.1344336980469771	0.8374689826302729
>10	0.3771612084095747	3.303349875930521
>50	0.0	0.0
>100	0.003734269390193809	0.8271298593879239
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT	320	0.8271298593879239	No Hit
ATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCTT	41	0.10597601323407775	Illumina PCR Primer Index 10 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0025847808105872623	0.0
13	0.0	0.0	0.0	0.0025847808105872623	0.0
14	0.0	0.0	0.0	0.0025847808105872623	0.0
15	0.0	0.0	0.0	0.0025847808105872623	0.0
16	0.0	0.0	0.0	0.0025847808105872623	0.0
17	0.0	0.0	0.0	0.0025847808105872623	0.0
18	0.0	0.0	0.0	0.0025847808105872623	0.0
19	0.0	0.0	0.0	0.007754342431761787	0.0
20	0.0	0.0	0.0	0.007754342431761787	0.0
21	0.0	0.0	0.0	0.01033912324234905	0.0
22	0.0	0.0	0.0	0.01033912324234905	0.0
23	0.0	0.0	0.0	0.01033912324234905	0.0
24	0.0	0.0	0.0	0.01033912324234905	0.0
25	0.0	0.0	0.0	0.01033912324234905	0.0
26	0.0	0.0	0.0	0.01033912324234905	0.0
27	0.0	0.0	0.0	0.01033912324234905	0.0
28	0.0	0.0	0.0	0.015508684863523574	0.0
29	0.0	0.0	0.0	0.02326302729528536	0.0
30	0.0	0.0	0.0	0.028432588916459885	0.0
31	0.0	0.0	0.0	0.031017369727047148	0.0
32	0.0	0.0	0.0	0.03877171215880893	0.0
33	0.0	0.0	0.0	0.051695616211745246	0.0
34	0.0	0.0	0.0	0.08012820512820513	0.0
35	0.0	0.0	0.0	0.10597601323407775	0.0
36	0.0	0.0	0.0	0.14733250620347393	0.0
37	0.0	0.0	0.0	0.23779983457402812	0.0
38	0.0	0.0	0.0	0.35928453267162946	0.0
39	0.0	0.0	0.0	0.5117866004962779	0.0
40	0.0	0.0	0.0	0.7237386269644334	0.0
41	0.0	0.0	0.0	0.9434449958643507	0.0
42	0.0	0.0	0.0	1.2251861042183623	0.0
43	0.0	0.0	0.0	1.566377171215881	0.0
44	0.0	0.0	0.0	1.9980355665839538	0.0
45	0.0	0.0	0.0	2.447787427626137	0.0
46	0.0	0.0	0.0	2.9544044665012406	0.0
47	0.0	0.0	0.0	3.406741108354012	0.0
48	0.0	0.0	0.0	3.9495450785773367	0.0
49	0.0	0.0	0.0	4.363110008271299	0.0
50	0.0	0.0	0.0	4.781844499586435	0.0
51	0.0	0.0	0.0	5.19282464846981	0.0
52	0.0	0.0	0.0	5.590880893300248	0.0
53	0.0	0.0	0.0	6.053556658395368	0.0
54	0.0	0.0	0.0	6.516232423490488	0.0
55	0.0	0.0	0.0	7.035773366418527	0.0
56	0.0	0.0	0.0	7.5242969396195205	0.0
57	0.0	0.0	0.0	8.087779156327544	0.0
58	0.0	0.0	0.0	8.53494623655914	0.0
59	0.0	0.0	0.0	9.01571546732837	0.0
60	0.0	0.0	0.0	9.504239040529363	0.0
61	0.0	0.0	0.0	9.974669148056245	0.0
62	0.0	0.0	0.0	10.398573200992557	0.0
63	0.0	0.0	0.0	10.892266335814723	0.0
64	0.0	0.0	0.0	11.49968982630273	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAATC	15	0.002208628	70.00001	13
TAGTGGT	15	0.002208628	70.00001	29
GGCAGGC	20	7.832232E-5	70.0	35
GCGCCAA	20	7.832232E-5	70.0	40
CACAAAT	20	7.832232E-5	70.0	37
CTGTGGT	20	7.832232E-5	70.0	14
TCCTGTG	25	2.3621444E-4	55.999996	12
CATTCGT	25	2.3621444E-4	55.999996	19
ATAGTCC	25	2.3621444E-4	55.999996	8
CGCCAAG	25	2.3621444E-4	55.999996	41
AACAAGA	20	0.0069015697	52.5	34
GTCGAAA	20	0.0069015697	52.5	11
TACCAAT	20	0.0069015697	52.5	41
GCTGGTA	20	0.0069015697	52.5	52
GAGTCGA	20	0.0069015697	52.5	9
TTCGTTT	20	0.0069015697	52.5	21
ATTCGTT	20	0.0069015697	52.5	20
ATGCTGG	20	0.0069015697	52.5	15
CAATTCG	20	0.0069015697	52.5	44
TGAGTCG	20	0.0069015697	52.5	8
>>END_MODULE