FastQCFastQC Report
Wed 25 May 2016
SRR1781189_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781189_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26004
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGAGCCCACTGTAAAGCTAACTTAGCATTAACCTTTTAAGTTAAAG390.14997692662667284No Hit
CTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAACAAGCTAACAT320.12305799107829565No Hit
CTCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCACAACTCAA310.1192124288570989No Hit
CCCTAGGAGGGCTGCCCCCGCTAACCGGCTTTTTGCCCAAATGGGCCATT280.1076757421935087No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCTG150.002200460570.0000137
TTGTTAT200.00687624352.565
TTGAGTC200.00687624352.552
CCCATCC200.00687624352.58
AACTTCG200.00687624352.533
AGGCCAG200.00687624352.559
TCTCCGC200.00687624352.519
CAAACTA200.00687624352.531
TACTCAT200.00687624352.524
ATACATT200.00687624352.511
CATACAT200.00687624352.510
TTTGTTA200.00687624352.564
TGAAACT200.00687624352.530
CTCCGAT200.00687624352.514
CGATCCG200.00687624352.517
TTTGAGT400.002376350635.051
CTAGGAG400.002376350635.035
AACAGCC400.002376350635.036
Produced by FastQC (version 0.11.5)