##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781189_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26004
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42947238886325	34.0	34.0	34.0	31.0	34.0
2	32.6211352099677	34.0	34.0	34.0	31.0	34.0
3	32.607060452238116	34.0	34.0	34.0	31.0	34.0
4	32.71558221812029	34.0	34.0	34.0	32.0	34.0
5	32.74842331948931	34.0	34.0	34.0	32.0	34.0
6	36.05056914320874	38.0	37.0	38.0	34.0	38.0
7	36.005076142131976	38.0	37.0	38.0	34.0	38.0
8	35.98161821258268	38.0	37.0	38.0	34.0	38.0
9	36.09187048146439	38.0	37.0	38.0	34.0	38.0
10-11	36.006845100753736	38.0	37.0	38.0	33.5	38.0
12-13	35.97967620366097	38.0	37.0	38.0	33.5	38.0
14-15	35.941739732348864	38.0	37.0	38.0	32.5	38.0
16-17	35.92393477926473	38.0	37.0	38.0	32.5	38.0
18-19	35.94983464082449	38.0	37.0	38.0	32.5	38.0
20-21	35.91491693585603	38.0	37.0	38.0	32.5	38.0
22-23	35.92306952776496	38.0	37.0	38.0	32.5	38.0
24-25	35.9656399015536	38.0	37.0	38.0	33.0	38.0
26-27	35.88328718658668	38.0	37.0	38.0	32.0	38.0
28-29	35.96771650515305	38.0	37.0	38.0	33.5	38.0
30-31	36.00796031379788	38.0	37.0	38.0	34.0	38.0
32-33	35.975292262728814	38.0	37.0	38.0	33.0	38.0
34-35	36.015920627595754	38.0	37.0	38.0	34.0	38.0
36-37	35.93568297185048	38.0	37.0	38.0	32.5	38.0
38-39	35.94531610521458	38.0	37.0	38.0	32.5	38.0
40-41	35.929491616674355	38.0	37.0	38.0	32.5	38.0
42-43	35.98463697892632	38.0	37.0	38.0	34.0	38.0
44-45	35.97210044608522	38.0	37.0	38.0	34.0	38.0
46-47	35.952468850946005	38.0	37.0	38.0	33.5	38.0
48-49	35.96204430087679	38.0	37.0	38.0	33.5	38.0
50-51	35.87705737578834	38.0	37.0	38.0	32.0	38.0
52-53	35.94691201353638	38.0	37.0	38.0	33.5	38.0
54-55	35.8926511305953	38.0	37.0	38.0	32.5	38.0
56-57	35.82571912013536	38.0	37.0	38.0	32.0	38.0
58-59	35.881383633287186	38.0	37.0	38.0	32.0	38.0
60-61	35.85919473927088	38.0	37.0	38.0	32.0	38.0
62-63	35.80137671127519	38.0	37.0	38.0	31.5	38.0
64-65	35.85323411782802	38.0	37.0	38.0	32.0	38.0
66-67	35.790013074911556	38.0	37.0	38.0	31.0	38.0
68-69	35.50463390247654	38.0	37.0	38.0	30.5	38.0
70-71	35.68183740962929	38.0	37.0	38.0	31.0	38.0
72-73	35.706506691278264	38.0	37.0	38.0	31.0	38.0
74-75	35.718754806952774	38.0	37.0	38.0	31.0	38.0
76	34.73634825411475	38.0	35.0	38.0	27.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	7.0
14	24.0
15	63.0
16	74.0
17	133.0
18	139.0
19	126.0
20	117.0
21	114.0
22	104.0
23	99.0
24	125.0
25	137.0
26	157.0
27	165.0
28	206.0
29	269.0
30	314.0
31	388.0
32	543.0
33	728.0
34	982.0
35	1735.0
36	3515.0
37	15740.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.56283648669435	19.950776803568683	9.975388401784341	28.510998307952622
2	21.696662051992003	20.42378095677588	31.902784187048145	25.976772804183973
3	17.974157821873558	21.692816489770806	28.945546838947855	31.387478849407785
4	13.0902938009537	17.108906322104293	36.50592216582064	33.294877711121366
5	12.494231656668205	34.05629903091832	35.80602984156284	17.643439470850637
6	27.557298877095832	38.74403937855714	18.527918781725887	15.170742962621137
7	26.445931395169975	30.41070604522381	22.41578218735579	20.727580372250422
8	23.48869404706968	34.96000615289955	19.604676203660976	21.94662359636979
9	22.538840178434086	15.712967235809874	21.300569143208737	40.4476234425473
10-11	23.357944931548992	25.27303491770497	27.472696508229504	23.89632364251654
12-13	23.15989847715736	23.259883094908478	26.688201815105366	26.892016612828794
14-15	21.738963236425164	24.615443777880326	24.059760036917396	29.585832948777114
16-17	21.848561759729275	27.188124903860945	25.4710813720966	25.492231964313184
18-19	21.442854945393016	27.599600061528996	26.2978772496539	24.659667743424087
20-21	21.733194893093373	27.514997692662668	26.232502691893554	24.51930472235041
22-23	21.47361944316259	27.1265959083218	26.430549146285188	24.96923550223043
24-25	20.452622673434856	27.707275803722503	26.56514382402707	25.274957698815566
26-27	20.46992770343024	27.372711890478392	27.17081987386556	24.986540532225813
28-29	20.61221350561452	27.216966620519923	27.8303337948008	24.34048607906476
30-31	21.2294262421166	27.420781418243344	27.072758037225043	24.27703430241501
32-33	20.791032148900168	27.614982310413783	26.999692355022304	24.594293185663744
34-35	21.16982002768805	26.753576372865712	27.48807875711429	24.58852484233195
36-37	21.85240732195047	26.57475772958006	26.895862175049995	24.67697277341947
38-39	20.78910936778957	27.79380095369943	27.4746192893401	23.942470389170897
40-41	20.852561144439317	26.52091985848331	27.838024919243193	24.78849407783418
42-43	20.827564990001537	27.372711890478392	27.426549761575142	24.373173357944932
44-45	20.671819720043068	27.730349177049685	26.963159513920935	24.634671588986308
46-47	20.983310259960007	26.928549453930167	27.111213659437013	24.976926626672817
48-49	21.065989847715734	27.21888940163052	26.820873711736652	24.89424703891709
50-51	20.78910936778957	27.40732195046916	26.803568681741268	25.0
52-53	20.744885402245806	26.901630518381786	27.19389324719274	25.159590832179664
54-55	20.521842793416397	26.968927857252726	27.27657283494847	25.232656514382402
56-57	19.995000769112444	27.249653899400094	27.609213967081985	25.146131364405477
58-59	20.687201968927855	26.955468389478543	27.078526380556838	25.278803261036764
60-61	20.429549300107674	26.50746039070912	27.395785263805568	25.667205045377635
62-63	19.570066143670203	26.628595600676817	28.209121673588676	25.592216582064296
64-65	20.56798954007076	27.176588217197356	27.165051530533763	25.090370712198123
66-67	20.03537917243501	26.597831102907243	27.578449469312417	25.788340255345332
68-69	19.83925549915398	26.115213044147055	27.463082602676515	26.582448854022456
70-71	20.691047531149053	26.89970773727119	27.413090293800952	24.996154437778802
72-73	20.61221350561452	25.859483156437474	27.05545300722966	26.47285033071835
74-75	21.052530379941548	25.905629903091832	27.11313644054761	25.928703276419014
76	21.185202276572834	26.107521919704663	26.761267497308104	25.9460083064144
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	2.0
16	5.0
17	7.0
18	8.5
19	10.0
20	15.5
21	21.0
22	29.0
23	45.5
24	66.5
25	79.0
26	104.0
27	142.5
28	195.0
29	234.0
30	259.5
31	322.0
32	432.0
33	505.0
34	555.0
35	694.0
36	890.5
37	998.0
38	1046.5
39	1253.0
40	1469.5
41	1588.5
42	1649.0
43	1629.5
44	1586.0
45	1572.5
46	1583.0
47	1544.0
48	1424.5
49	1330.5
50	1317.0
51	1183.5
52	960.5
53	778.5
54	686.0
55	665.0
56	581.5
57	485.5
58	452.0
59	383.5
60	300.5
61	240.0
62	194.0
63	197.0
64	201.5
65	195.5
66	177.5
67	167.0
68	187.0
69	193.0
70	160.0
71	141.0
72	161.0
73	155.0
74	115.0
75	101.0
76	104.5
77	90.0
78	73.0
79	74.0
80	62.5
81	45.0
82	37.0
83	35.0
84	23.0
85	9.0
86	7.0
87	7.0
88	5.5
89	2.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	26004.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.45900630672205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.3453304015739	50.73834794646977
2	13.632681402615438	18.120289186279035
3	4.501793773868766	8.97554222427319
4	2.065733132739266	5.491462851868943
5	1.2556417081356326	4.172435009998462
6	0.8100914246036338	3.2302722658052603
7	0.4050457123018169	1.884325488386402
8	0.34139567179724567	1.8151053684048606
9	0.17359101955792153	1.0383017997231194
>10	0.4686957528063881	4.533917858790955
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCTAGAGCCCACTGTAAAGCTAACTTAGCATTAACCTTTTAAGTTAAAG	39	0.14997692662667284	No Hit
CTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAACAAGCTAACAT	32	0.12305799107829565	No Hit
CTCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCACAACTCAA	31	0.1192124288570989	No Hit
CCCTAGGAGGGCTGCCCCCGCTAACCGGCTTTTTGCCCAAATGGGCCATT	28	0.1076757421935087	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.003845562221196739	0.0
22	0.0	0.0	0.0	0.03845562221196739	0.0
23	0.0	0.0	0.0	0.046146746654360866	0.0
24	0.0	0.0	0.0	0.049992308875557605	0.0
25	0.0	0.0	0.0	0.049992308875557605	0.0
26	0.0	0.0	0.0	0.049992308875557605	0.0
27	0.0	0.0	0.0	0.049992308875557605	0.0
28	0.0	0.0	0.0	0.05383787109675434	0.0
29	0.0	0.0	0.0	0.05383787109675434	0.0
30	0.0	0.0	0.0	0.05768343331795108	0.0
31	0.0	0.0	0.0	0.06537455776034456	0.0
32	0.0	0.0	0.0	0.06537455776034456	0.0
33	0.0	0.0	0.0	0.08075680664513152	0.0
34	0.0	0.0	0.0	0.09229349330872173	0.0
35	0.0	0.0	0.0	0.09998461775111521	0.0
36	0.0	0.0	0.0	0.11536686663590216	0.0
37	0.0	0.0	0.0	0.18074142439624674	0.0
38	0.0	0.0	0.0	0.24996154437778803	0.0
39	0.0	0.0	0.0	0.3191816643593293	0.0
40	0.0	0.0	0.0	0.4114751576680511	0.0
41	0.0	0.0	0.0	0.48069527764959236	0.0
42	0.0	0.0	0.0	0.5922165820642978	0.0
43	0.0	0.0	0.0	0.796031379787725	0.0
44	0.0	0.0	0.0	0.9498538686355945	0.0
45	0.0	0.0	0.0	1.1805876019073989	0.0
46	0.0	0.0	0.0	1.4382402707275803	0.0
47	0.0	0.0	0.0	1.7766497461928934	0.0
48	0.0	0.0	0.0	2.065066912782649	0.0
49	0.0	0.0	0.0	2.3304107060452237	0.0
50	0.0	0.0	0.0	2.568835563759422	0.0
51	0.0	0.0	0.0	2.8687894170127675	0.0
52	0.0	0.0	0.0	3.1187509613905555	0.0
53	0.0	0.0	0.0	3.37255806798954	0.0
54	0.0	0.0	0.0	3.6455929856945084	0.0
55	0.0	0.0	0.0	4.007075834487002	0.0
56	0.0	0.0	0.0	4.372404245500692	0.0
57	0.0	0.0	0.0	4.760806029841563	0.0
58	0.0	0.0	0.0	5.137671127518844	0.0
59	0.0	0.0	0.0	5.48377172742655	0.0
60	0.0	0.0	0.0	5.799107829564682	0.0
61	0.0	0.0	0.0	6.17981848946316	0.0
62	0.0	0.0	0.0	6.575911398246424	0.0
63	0.0	0.0	0.0	6.879710813720966	0.0
64	0.0	0.0	0.0	7.237348100292262	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAATCTG	15	0.0022004605	70.00001	37
TTGTTAT	20	0.006876243	52.5	65
TTGAGTC	20	0.006876243	52.5	52
CCCATCC	20	0.006876243	52.5	8
AACTTCG	20	0.006876243	52.5	33
AGGCCAG	20	0.006876243	52.5	59
TCTCCGC	20	0.006876243	52.5	19
CAAACTA	20	0.006876243	52.5	31
TACTCAT	20	0.006876243	52.5	24
ATACATT	20	0.006876243	52.5	11
CATACAT	20	0.006876243	52.5	10
TTTGTTA	20	0.006876243	52.5	64
TGAAACT	20	0.006876243	52.5	30
CTCCGAT	20	0.006876243	52.5	14
CGATCCG	20	0.006876243	52.5	17
TTTGAGT	40	0.0023763506	35.0	51
CTAGGAG	40	0.0023763506	35.0	35
AACAGCC	40	0.0023763506	35.0	36
>>END_MODULE