FastQCFastQC Report
Wed 25 May 2016
SRR1781189_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781189_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24638
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT1000.4058771004139946No Hit
ATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCTT510.2069973212111373RNA PCR Primer, Index 32 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTTGA150.002199042170.0000125
AATCTCG252.3451801E-456.00000436
ATCTCGT252.3451801E-456.00000437
TCTCGTA252.3451801E-456.00000438
CTATACA200.006871843252.51
CGTATGC352.0279402E-550.00000441
GCCGTCT352.0279402E-550.00000446
TGCCGTC404.464397E-543.7545
TATGCCG404.464397E-543.7543
CAATCTT404.464397E-543.7529
CCGTCTT404.464397E-543.7547
TCGTATG404.464397E-543.7540
CTCGTAT404.464397E-543.7539
TTCTGCT350.001231970840.00000452
TAATCTC350.001231970840.00000435
CTGCTTG350.001231970840.00000454
TCTTCTG350.001231970840.00000450
CGTCTTC350.001231970840.00000448
CTTCTGC350.001231970840.00000451
ATGCCGT458.942012E-538.88888544