##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781189_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24638
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.879251562626838	34.0	33.0	34.0	27.0	34.0
2	32.475444435424954	34.0	34.0	34.0	27.0	34.0
3	32.69567335010959	34.0	34.0	34.0	31.0	34.0
4	32.94788538030684	34.0	34.0	34.0	31.0	34.0
5	32.76597126390129	34.0	34.0	34.0	31.0	34.0
6	35.729807614254405	37.0	36.0	38.0	31.0	38.0
7	35.675338907378844	37.0	36.0	38.0	31.0	38.0
8	35.20565792677977	37.0	35.0	38.0	27.0	38.0
9	35.41095056416917	37.0	36.0	38.0	31.0	38.0
10-11	35.2854939524312	37.0	35.0	38.0	30.5	38.0
12-13	35.258685769948855	37.0	35.0	38.0	29.5	38.0
14-15	35.35256514327462	37.0	35.0	38.0	31.0	38.0
16-17	35.244967123954865	37.0	35.0	38.0	30.0	38.0
18-19	35.21361311794789	37.0	35.0	38.0	29.5	38.0
20-21	35.11114944394838	37.0	35.0	38.0	27.0	38.0
22-23	35.1775509375761	37.0	35.0	38.0	29.5	38.0
24-25	35.19713450767108	37.0	35.0	38.0	29.0	38.0
26-27	34.903583894796654	37.0	34.5	38.0	27.0	38.0
28-29	34.8081418946343	37.0	34.0	38.0	27.0	38.0
30-31	34.825493140677004	37.0	34.0	38.0	27.0	38.0
32-33	34.80073463755175	37.0	34.5	38.0	27.0	38.0
34-35	34.73122818410586	37.0	34.0	38.0	27.0	38.0
36-37	34.712314311226564	37.0	34.0	38.0	27.0	38.0
38-39	34.733176394187836	37.0	34.0	38.0	27.0	38.0
40-41	34.73776280542252	37.0	34.0	38.0	27.0	38.0
42-43	34.69009253997889	37.0	34.0	38.0	27.0	38.0
44-45	34.61183943501908	37.0	34.0	38.0	27.0	38.0
46-47	34.64495900641286	37.0	34.0	38.0	27.0	38.0
48-49	34.634954135887654	37.0	34.0	38.0	27.0	38.0
50-51	34.52408880590957	37.0	34.0	38.0	27.0	38.0
52-53	34.507711664907866	37.0	34.0	38.0	27.0	38.0
54-55	34.50385583245394	37.0	34.0	38.0	26.5	38.0
56-57	34.46192872798117	37.0	34.0	38.0	26.5	38.0
58-59	34.48321698189788	37.0	34.0	38.0	26.5	38.0
60-61	34.453486484292554	37.0	34.0	38.0	26.5	38.0
62-63	34.32557431609709	37.0	34.0	38.0	25.0	38.0
64-65	34.286143355791864	37.0	34.0	38.0	25.0	38.0
66-67	34.29306356035393	37.0	34.0	38.0	25.0	38.0
68-69	34.15220391265525	37.0	34.0	38.0	24.5	38.0
70-71	34.160828801039045	37.0	34.0	38.0	24.5	38.0
72-73	34.11370646968098	37.0	34.0	38.0	24.0	38.0
74-75	34.169555158697946	37.0	34.0	38.0	24.0	38.0
76	33.021673837162105	37.0	32.0	38.0	22.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	2.0
21	0.0
22	21.0
23	28.0
24	68.0
25	97.0
26	193.0
27	329.0
28	462.0
29	666.0
30	1003.0
31	1380.0
32	1773.0
33	2252.0
34	2921.0
35	3654.0
36	4767.0
37	5020.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.25375436317883	20.52926373893985	9.741050409935871	23.475931487945452
2	24.669210163162596	22.721000081175422	30.18913872879292	22.420651026869066
3	21.604838055036936	24.851854858348894	28.529101388099683	25.01420569851449
4	15.89414725221203	18.75963958113483	37.30822307005439	28.037990096598747
5	15.524799090835295	35.45336472116244	34.58072895527234	14.44110723272993
6	31.337770922964523	38.485266661254975	17.473009172822472	12.703953242958033
7	30.209432583813623	30.554428119165518	21.633249452065915	17.602889844954948
8	25.821901128338336	35.985063722704766	19.409042941797225	18.78399220715967
9	26.775712314311228	16.235084016559785	21.523662634954135	35.465541034174855
10-11	26.382011526909654	25.85640068187353	27.48802662553779	20.273561165679034
12-13	26.54842113807939	22.936114944394838	26.934004383472686	23.58145953405309
14-15	24.90056011039857	24.255215520740318	23.827015179803556	27.017209189057557
16-17	24.831561003328193	27.638201152690968	25.24149687474633	22.288740969234517
18-19	24.35871418134589	26.897475444435425	26.651919798684958	22.09189057553373
20-21	24.626593067619122	27.074031983115514	26.41854046594691	21.880834483318452
22-23	24.811267148307493	27.03547365857618	26.306924263333066	21.84633492978326
24-25	24.38103742186866	27.36220472440945	26.40433476743242	21.85242308628947
26-27	24.376978650864515	27.548908190599885	26.491598344021426	21.582514814514163
28-29	24.14968747463268	26.787888627323646	27.936520821495254	21.125903076548422
30-31	23.824985794301483	27.258706063803878	27.514408637064697	21.401899504829938
32-33	23.250669697215685	27.376410422923943	27.427145060475688	21.945774819384688
34-35	24.853884243850963	26.02686906404741	28.123224287685687	20.996022404415942
36-37	24.650945693643962	26.733095218767755	27.08823768163	21.527721405958275
38-39	23.820927023297344	27.11867846416105	27.790405065346214	21.26998944719539
40-41	23.997483561977432	26.327218118353763	28.009578699569772	21.665719620099033
42-43	24.43583083042455	26.246042698270966	27.91622696647455	21.401899504829938
44-45	23.916308141894635	27.289146846334926	27.230294666774903	21.564250344995536
46-47	24.389154963876937	26.089780014611573	27.262764834808017	22.258300186703465
48-49	23.806721324782856	26.0613686175826	27.8654923289228	22.266417728711748
50-51	23.912249370890496	26.649890413182888	27.15520740319831	22.28265281272831
52-53	23.822956408799413	26.110073869632277	28.307898368374058	21.759071353194255
54-55	23.173553048137023	26.33939443136618	28.218605406282975	22.268447114213817
56-57	23.693075736666938	25.982222583001867	27.554996347106098	22.769705333225097
58-59	23.329815731796412	26.205454988229565	28.486484292556213	21.97824498741781
60-61	23.477960873447522	26.66815488270152	27.786346294342074	22.06753794950889
62-63	22.63779527559055	27.086208296127936	28.141488757204318	22.1345076710772
64-65	23.18572936114944	27.447438915496384	27.364234109911518	22.00259761344265
66-67	23.122818410585275	26.881240360418868	27.408880590957057	22.587060638038803
68-69	22.434856725383554	27.0679438266093	28.07046026463187	22.426739183375275
70-71	22.871174608328598	26.871093432908516	27.40076304894878	22.856968909814107
72-73	22.463268122412533	26.5342154395649	27.552966961604024	23.44954947641854
74-75	22.769705333225097	26.333306274859975	27.000974105040992	23.896014286873935
76	22.980761425440377	25.959899342479098	27.774169981329656	23.285169250750872
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	2.0
15	2.0
16	4.0
17	6.0
18	9.0
19	15.0
20	22.5
21	27.0
22	30.0
23	39.0
24	67.0
25	89.0
26	106.5
27	128.5
28	169.5
29	206.0
30	230.0
31	294.0
32	391.5
33	449.0
34	545.0
35	680.5
36	786.0
37	852.0
38	946.5
39	1144.0
40	1384.0
41	1528.5
42	1536.0
43	1578.5
44	1593.0
45	1601.0
46	1637.0
47	1544.5
48	1404.0
49	1256.0
50	1156.0
51	1041.0
52	866.5
53	764.0
54	721.0
55	685.0
56	567.5
57	435.0
58	384.0
59	328.5
60	256.0
61	196.0
62	153.0
63	161.0
64	174.5
65	177.5
66	170.0
67	165.0
68	168.5
69	158.5
70	150.5
71	156.0
72	155.5
73	151.0
74	132.5
75	118.0
76	96.5
77	78.0
78	72.0
79	63.0
80	50.0
81	35.0
82	26.5
83	20.0
84	16.5
85	7.0
86	2.0
87	3.0
88	2.5
89	3.0
90	2.5
91	1.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	24638.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.51051221690072
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.14434193623731	54.3185323484049
2	12.670792946397292	17.61506615796737
3	4.542800420413407	9.473171523662636
4	2.014480906224454	5.601103985713126
5	1.0276772159289969	3.571718483643153
6	0.5196776830550042	2.1673837162107317
7	0.34450543033983416	1.6762724247097978
8	0.26859745416326053	1.4936277295235003
9	0.16933317762466424	1.0593392320805262
>10	0.28611467943477753	2.410909976459128
>50	0.01167815018101133	0.612874421625132
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT	100	0.4058771004139946	No Hit
ATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCTT	51	0.2069973212111373	RNA PCR Primer, Index 32 (95% over 21bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.004058771004139946	0.0
29	0.0	0.0	0.0	0.004058771004139946	0.0
30	0.0	0.0	0.0	0.012176313012419839	0.0
31	0.0	0.0	0.0	0.020293855020699733	0.0
32	0.0	0.0	0.0	0.020293855020699733	0.0
33	0.0	0.0	0.0	0.03652893903725952	0.0
34	0.0	0.0	0.0	0.048705252049679355	0.0
35	0.0	0.0	0.0	0.0608815650620992	0.0
36	0.0	0.0	0.0	0.08523419108693887	0.0
37	0.0	0.0	0.0	0.17858592418215763	0.0
38	0.0	0.0	0.0	0.25570257326081663	0.0
39	0.0	0.0	0.0	0.32064290932705575	0.0
40	0.0	0.0	0.0	0.4180534134264145	0.0
41	0.0	0.0	0.0	0.4829937494926536	0.0
42	0.0	0.0	0.0	0.5844630245961523	0.0
43	0.0	0.0	0.0	0.7671077197824498	0.0
44	0.0	0.0	0.0	0.9132234759314879	0.0
45	0.0	0.0	0.0	1.1039857131260655	0.0
46	0.0	0.0	0.0	1.3515707443786023	0.0
47	0.0	0.0	0.0	1.651919798684958	0.0
48	0.0	0.0	0.0	1.9441513109830344	0.0
49	0.0	0.0	0.0	2.191736342235571	0.0
50	0.0	0.0	0.0	2.414968747463268	0.0
51	0.0	0.0	0.0	2.7153178017696242	0.0
52	0.0	0.0	0.0	2.966961604026301	0.0
53	0.0	0.0	0.0	3.1942527802581377	0.0
54	0.0	0.0	0.0	3.4458965825148145	0.0
55	0.0	0.0	0.0	3.79900965987499	0.0
56	0.0	0.0	0.0	4.135887653218606	0.0
57	0.0	0.0	0.0	4.493059501582921	0.0
58	0.0	0.0	0.0	4.862407662959656	0.0
59	0.0	0.0	0.0	5.178991801282572	0.0
60	0.0	0.0	0.0	5.463105771572368	0.0
61	0.0	0.0	0.0	5.860865329978083	0.0
62	0.0	0.0	0.0	6.250507346375517	0.0
63	0.0	0.0	0.0	6.526503774657034	0.0
64	0.0	0.0	0.0	6.85526422599237	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATTTGA	15	0.0021990421	70.00001	25
AATCTCG	25	2.3451801E-4	56.000004	36
ATCTCGT	25	2.3451801E-4	56.000004	37
TCTCGTA	25	2.3451801E-4	56.000004	38
CTATACA	20	0.0068718432	52.5	1
CGTATGC	35	2.0279402E-5	50.000004	41
GCCGTCT	35	2.0279402E-5	50.000004	46
TGCCGTC	40	4.464397E-5	43.75	45
TATGCCG	40	4.464397E-5	43.75	43
CAATCTT	40	4.464397E-5	43.75	29
CCGTCTT	40	4.464397E-5	43.75	47
TCGTATG	40	4.464397E-5	43.75	40
CTCGTAT	40	4.464397E-5	43.75	39
TTCTGCT	35	0.0012319708	40.000004	52
TAATCTC	35	0.0012319708	40.000004	35
CTGCTTG	35	0.0012319708	40.000004	54
TCTTCTG	35	0.0012319708	40.000004	50
CGTCTTC	35	0.0012319708	40.000004	48
CTTCTGC	35	0.0012319708	40.000004	51
ATGCCGT	45	8.942012E-5	38.888885	44
>>END_MODULE