FastQCFastQC Report
Wed 25 May 2016
SRR1781188_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781188_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171099
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACGT200.006941976552.513
TGGTACG200.006941976552.521
GTCATAG305.8653025E-446.6666643
TTAACGG305.8653025E-446.66666435
CAATCGA350.001252775240.021
CCGTGCA400.002413700635.056
TGGTTAC450.004298314531.11111340
CAGTCAT450.004298314531.1111131
AGTACCG450.004298314531.1111135
GTACCGC500.007193492327.9999986
GGTATAA500.007193492327.9999981
TATAGTG500.007193492327.9999985
GCTTTAA500.007193492327.99999846
GCTATAC657.93152E-426.9230771
GATATAA657.93152E-426.9230771
TGTTTGC700.001222290225.056
ATACTGG700.001222290225.06
TATACTG1001.389733E-524.4999985
GGTTTAA901.9195501E-423.3333341
ACATGTT750.001826689223.33333429
Produced by FastQC (version 0.11.5)