##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781188_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	171099
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.793932167926172	34.0	34.0	34.0	24.0	34.0
2	32.04352450920228	34.0	34.0	34.0	27.0	34.0
3	32.03118077837977	34.0	34.0	34.0	27.0	34.0
4	32.176003366472045	34.0	34.0	34.0	27.0	34.0
5	32.27097762114332	34.0	34.0	34.0	27.0	34.0
6	35.26525578758496	38.0	36.0	38.0	27.0	38.0
7	35.10576917457145	38.0	36.0	38.0	27.0	38.0
8	34.926194776123765	38.0	36.0	38.0	27.0	38.0
9	35.27557729735416	38.0	36.0	38.0	27.0	38.0
10-11	35.150193747479534	38.0	36.0	38.0	27.0	38.0
12-13	35.13024915399856	38.0	36.0	38.0	27.0	38.0
14-15	35.09101748110743	38.0	36.0	38.0	27.0	38.0
16-17	35.073068808116936	38.0	36.0	38.0	27.0	38.0
18-19	35.09237342123566	38.0	36.0	38.0	27.0	38.0
20-21	35.072309014079565	38.0	36.0	38.0	27.0	38.0
22-23	35.10451843669455	38.0	36.0	38.0	27.0	38.0
24-25	35.10971133671149	38.0	36.0	38.0	27.0	38.0
26-27	35.06437501095857	38.0	36.0	38.0	27.0	38.0
28-29	35.15859239387723	38.0	36.0	38.0	27.0	38.0
30-31	35.214630126418044	38.0	36.0	38.0	27.0	38.0
32-33	35.18016762225378	38.0	36.0	38.0	27.0	38.0
34-35	35.200708361825605	38.0	36.0	38.0	27.0	38.0
36-37	35.10120164349295	38.0	36.0	38.0	27.0	38.0
38-39	35.09168083974775	38.0	36.0	38.0	27.0	38.0
40-41	35.08750489482698	38.0	36.0	38.0	27.0	38.0
42-43	35.17585725223408	38.0	36.0	38.0	27.0	38.0
44-45	35.159185617683335	38.0	36.0	38.0	27.0	38.0
46-47	35.111806614883776	38.0	36.0	38.0	27.0	38.0
48-49	35.0901758630968	38.0	36.0	38.0	27.0	38.0
50-51	35.010876743873425	38.0	36.0	38.0	27.0	38.0
52-53	35.040999655170395	38.0	36.0	38.0	27.0	38.0
54-55	35.00597315004764	38.0	36.0	38.0	27.0	38.0
56-57	34.932647180871896	38.0	36.0	38.0	27.0	38.0
58-59	34.963883482662084	38.0	36.0	38.0	27.0	38.0
60-61	34.944891554012585	38.0	36.0	38.0	27.0	38.0
62-63	34.900808888421324	38.0	36.0	38.0	27.0	38.0
64-65	34.93496163040111	38.0	36.0	38.0	27.0	38.0
66-67	34.828733657122484	38.0	36.0	38.0	27.0	38.0
68-69	34.56430487612435	38.0	35.0	38.0	25.0	38.0
70-71	34.74801430750618	38.0	35.0	38.0	26.5	38.0
72-73	34.75845563095051	38.0	35.0	38.0	27.0	38.0
74-75	34.76541651324672	38.0	35.5	38.0	27.0	38.0
76	33.58473164659057	37.0	34.0	38.0	23.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	16.0
14	63.0
15	173.0
16	350.0
17	484.0
18	641.0
19	810.0
20	878.0
21	924.0
22	1089.0
23	1214.0
24	1371.0
25	1675.0
26	1943.0
27	2297.0
28	2752.0
29	3374.0
30	3954.0
31	4908.0
32	6181.0
33	8010.0
34	10942.0
35	16400.0
36	28935.0
37	71714.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.83254139416361	17.895487407874974	8.729449032431516	25.542522165529896
2	23.3671733908439	21.01414970280364	30.937644287809977	24.68103261854248
3	19.600348336343288	21.599191111578676	29.728987311439575	29.07147324063846
4	13.609664580155348	16.969707596187003	36.14632464245846	33.27430318119919
5	14.080152426373035	32.65185652750747	35.65304297511967	17.61494807099983
6	30.007188820507423	36.74305519026996	18.637747736690454	14.61200825253216
7	28.28479418348441	29.855230013033392	21.492819946346852	20.367155857135344
8	25.11353076289166	34.779864289095784	20.13980210287611	19.966802845136442
9	24.31691593755662	16.685661517600924	21.063828543708613	37.93359400113385
10-11	24.67869479073519	24.41831921869795	27.668484327786835	23.234501662780026
12-13	25.04982495514293	22.64595351229405	27.027627280112682	25.276594252450334
14-15	23.00948573632809	23.902535958713962	24.340294215629548	28.747684089328402
16-17	22.95074781266986	27.318979070596555	25.026738905545916	24.703534211187677
18-19	22.491656877012723	27.020029339738983	26.581978854347483	23.90633492890081
20-21	22.801711289954937	26.923886171164064	26.351995043805047	23.92240749507595
22-23	22.779209697309742	26.750302456472568	26.509506192321403	23.960981653896283
24-25	22.802587975382675	26.700915844043504	26.331539050491237	24.164957130082584
26-27	22.176050123028187	26.478237745398864	26.816638320504506	24.529073811068447
28-29	22.44139357915593	26.498985967188588	27.18718402796042	23.872436425695064
30-31	22.626958661359797	26.97561061140042	26.71202052612815	23.685410201111637
32-33	21.965937848847744	27.129615018205833	26.8794674428255	24.024979690120922
34-35	22.49282579091637	26.335630249153997	27.187476256436334	23.9840677034933
36-37	22.870384981794164	26.716696181742734	26.477068831495217	23.935850004967886
38-39	22.391780173408886	26.838633886328633	26.92104255735731	23.84854338290517
40-41	22.076107984266418	26.53989795381621	27.42125903716562	23.96273502475175
42-43	22.12023448412907	26.64977586075898	27.066785895884838	24.163203759227116
44-45	22.21637765270399	26.539313496864388	27.150071011519643	24.094237838911976
46-47	22.055067534000784	26.558600576274554	27.189521855767712	24.196810033956947
48-49	21.74705872038995	26.388523603294	27.769595380452255	24.0948222958638
50-51	22.0126944049936	26.391153659577206	27.508343122987277	24.087808812441917
52-53	21.988731669968846	26.3759577788298	27.53815042753026	24.09716012367109
54-55	21.643902068393153	26.626689811161956	27.582569155868825	24.146838964576066
56-57	21.711991303280556	26.51593521879146	27.687771407197005	24.08430207073098
58-59	21.689489710635364	26.236272567344056	27.807585082320763	24.266652639699824
60-61	21.551850098480998	26.324525567069358	27.5945505233812	24.529073811068447
62-63	21.262543907328503	26.35550178551599	27.928859899824076	24.453094407331427
64-65	21.51649045289569	26.230720226301727	27.764627496361754	24.48816182444082
66-67	21.438757678303205	26.302316202900077	27.45837205360639	24.800554065190326
68-69	21.524380621745305	26.315174255840184	27.271345829022962	24.889099293391546
70-71	21.980841501119237	26.357255156371455	27.102729998421964	24.55917334408734
72-73	21.96213887866089	25.8949497074793	27.22926492849169	24.913646485368123
74-75	22.131631394689624	25.994891846241064	26.92914628373047	24.944330475338838
76	22.34554263905692	25.78098060187377	26.701500300995328	25.17197645807398
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	1.0
9	0.5
10	1.5
11	4.0
12	5.0
13	5.5
14	11.0
15	27.5
16	72.0
17	105.0
18	133.0
19	204.5
20	331.0
21	414.0
22	490.5
23	696.0
24	1023.0
25	1221.0
26	1411.0
27	1771.0
28	2233.0
29	2525.0
30	2789.5
31	3309.5
32	3864.0
33	4163.0
34	4479.5
35	5237.0
36	5999.0
37	6320.0
38	6536.0
39	7238.5
40	7869.5
41	8146.0
42	8278.0
43	8263.5
44	8250.0
45	8143.0
46	8035.0
47	7852.5
48	7409.0
49	6791.0
50	6434.0
51	6148.0
52	5576.0
53	5051.5
54	4813.0
55	4651.5
56	4254.5
57	3799.0
58	3579.0
59	3398.5
60	3035.5
61	2638.5
62	2424.0
63	2291.5
64	2115.5
65	2025.5
66	1902.0
67	1825.0
68	1787.5
69	1718.0
70	1632.0
71	1578.0
72	1480.0
73	1332.5
74	1213.0
75	1143.0
76	1068.0
77	876.5
78	703.5
79	647.0
80	567.5
81	396.0
82	275.5
83	247.0
84	193.0
85	124.0
86	92.0
87	75.0
88	56.5
89	31.0
90	25.5
91	19.0
92	11.0
93	9.5
94	5.5
95	2.0
96	1.0
97	2.5
98	2.5
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	2.9222847591160674E-4
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	171099.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.4167391218256
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.30578080584894	50.83759220637323
2	17.613826401936812	24.101611317568697
3	4.482683275762811	9.200717167309044
4	1.4233535147277998	3.8952482438106175
5	0.6713357052692583	2.2965299905286827
6	0.39287179980968073	1.6127404461540613
7	0.27705062463850827	1.3268430216602154
8	0.20085519960888776	1.0993486234322785
9	0.14247747036563227	0.877305952866879
>10	0.4897652020316832	4.752063030296324
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0017533708554696404	0.0
11	0.0	0.0	0.0	0.0017533708554696404	0.0
12	0.0	0.0	0.0	0.0017533708554696404	0.0
13	0.0	0.0	0.0	0.0017533708554696404	0.0
14	0.0	0.0	0.0	0.0017533708554696404	0.0
15	0.0	0.0	0.0	0.0017533708554696404	0.0
16	0.0	0.0	0.0	0.002337827807292854	0.0
17	0.0	0.0	0.0	0.002337827807292854	0.0
18	0.0	0.0	0.0	0.002922284759116067	0.0
19	0.0	0.0	0.0	0.004091198662762494	0.0
20	0.0	0.0	0.0	0.004091198662762494	0.0
21	0.0	0.0	0.0	0.004675655614585708	0.0
22	0.0	0.0	0.0	0.005844569518232134	0.0
23	0.0	0.0	0.0	0.005844569518232134	0.0
24	0.0	0.0	0.0	0.007597940373701775	0.0
25	0.0	0.0	0.0	0.009351311229171416	0.0
26	0.0	0.0	0.0	0.009351311229171416	0.0
27	0.0	0.0	0.0	0.012858052940110696	0.0
28	0.0	0.0	0.0	0.015780337699226762	0.0
29	0.0	0.0	0.0	0.02045599331381247	0.0
30	0.0	0.0	0.0	0.024547191976574966	0.0
31	0.0	0.0	0.0	0.0303917614948071	0.0
32	0.0	0.0	0.0	0.03798970186850888	0.0
33	0.0	0.0	0.0	0.046756556145857074	0.0
34	0.0	0.0	0.0	0.05727678127867492	0.0
35	0.0	0.0	0.0	0.0824084302070731	0.0
36	0.0	0.0	0.0	0.1128001917018802	0.0
37	0.0	0.0	0.0	0.18527285372795868	0.0
38	0.0	0.0	0.0	0.27995487992331924	0.0
39	0.0	0.0	0.0	0.4038597537098405	0.0
40	0.0	0.0	0.0	0.5435449651955885	0.0
41	0.0	0.0	0.0	0.7060239978024418	0.0
42	0.0	0.0	0.0	0.876100970782997	0.0
43	0.0	0.0	0.0	1.0672183940291877	0.0
44	0.0	0.0	0.0	1.2589202742272019	0.0
45	0.0	0.0	0.0	1.4962097966674264	0.0
46	0.0	0.0	0.0	1.7855159878199172	0.0
47	0.0	0.0	0.0	2.094693715334397	0.0
48	0.0	0.0	0.0	2.4173139527408107	0.0
49	0.0	0.0	0.0	2.7428564749063407	0.0
50	0.0	0.0	0.0	3.0812570500119816	0.0
51	0.0	0.0	0.0	3.3740699828754113	0.0
52	0.0	0.0	0.0	3.642335723762266	0.0
53	0.0	0.0	0.0	3.893652213046248	0.0
54	0.0	0.0	0.0	4.196400914090673	0.0
55	0.0	0.0	0.0	4.516683323689794	0.0
56	0.0	0.0	0.0	4.85975955441002	0.0
57	0.0	0.0	0.0	5.195822301708367	0.0
58	0.0	0.0	0.0	5.559354525742407	0.0
59	0.0	0.0	0.0	5.901261842558986	0.0
60	0.0	0.0	0.0	6.263625152689379	0.0
61	0.0	0.0	0.0	6.620143893301539	0.0
62	0.0	0.0	0.0	6.936919561189721	0.0
63	0.0	0.0	0.0	7.260708712499781	0.0
64	0.0	0.0	0.0	7.574562095628846	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGACGT	20	0.0069419765	52.5	13
TGGTACG	20	0.0069419765	52.5	21
GTCATAG	30	5.8653025E-4	46.666664	3
TTAACGG	30	5.8653025E-4	46.666664	35
CAATCGA	35	0.0012527752	40.0	21
CCGTGCA	40	0.0024137006	35.0	56
TGGTTAC	45	0.0042983145	31.111113	40
CAGTCAT	45	0.0042983145	31.111113	1
AGTACCG	45	0.0042983145	31.111113	5
GTACCGC	50	0.0071934923	27.999998	6
GGTATAA	50	0.0071934923	27.999998	1
TATAGTG	50	0.0071934923	27.999998	5
GCTTTAA	50	0.0071934923	27.999998	46
GCTATAC	65	7.93152E-4	26.923077	1
GATATAA	65	7.93152E-4	26.923077	1
TGTTTGC	70	0.0012222902	25.0	56
ATACTGG	70	0.0012222902	25.0	6
TATACTG	100	1.389733E-5	24.499998	5
GGTTTAA	90	1.9195501E-4	23.333334	1
ACATGTT	75	0.0018266892	23.333334	29
>>END_MODULE