FastQCFastQC Report
Wed 25 May 2016
SRR1781188_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781188_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160415
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT6530.4070691643549543No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCAA150.002221406470.012
TAGTACT252.3848179E-456.0000044
CTCGTAT1250.056.039
TATGCCG1250.056.043
CGTATGC1300.053.84615341
CCGTCTT1250.053.19999747
GTGTTCG200.006941192352.511
CATCGTT200.006941192352.532
TCGACTC200.006941192352.550
TTCGACT200.006941192352.549
CAATACG200.006941192352.569
CGTCTTC1300.051.15384748
GCCGTCT1300.051.15384746
TCGTATG1400.050.00000440
TCTCGTA1050.049.99999638
TGCCGTC1350.049.2592645
ATGCCGT1450.048.2758644
GTATGCC1500.046.66666842
ATCTCGT1150.045.65217637
ACACCGT350.001252541440.00000429
Produced by FastQC (version 0.11.5)